Volume 1, Issue 3 (7-2016)                   J Res Dent Maxillofac Sci 2016, 1(3): 1-7 | Back to browse issues page

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Vatanpour M, Falah Doust A, Shojaee G, Jamshidian A, Farasat A. In Vitro Comparison of the Effect of Ledermix® Paste and Green Tea Extract on the Concentration of Inflammatory Mediators. J Res Dent Maxillofac Sci 2016; 1 (3) :1-7
URL: http://jrdms.dentaliau.ac.ir/article-1-110-en.html
1- Assistant Professor, Endodontics Dept, Dental Branch of Tehran, Islamic Azad University, Tehran, Iran
2- Post Graduate student, Endodontics Dept, Dental faculty,Qom university of medical sciences,Qom,Iran
3- Post Graduate student,Endodontics Dept, Dental Branch of Tehran, Islamic Azad University, Tehran, Iran. , jmdn.ali@gmail.com
4- Pharmaceutical Sciences Branch,Islamic Azad University,Tehran,Iran
Abstract:   (6039 Views)

Background and aim: The purpose of this study is to compare the effect of Ledermix® paste and green tea extract on the concentration of inflammatory mediators.

Materials and methods: In this in-vitro experimental study, first, the noncytotoxic concentrations of Ledermix® medicament and green tea extract were determined by MTT assay. Afterwards, the concentrations of IL-1β, IL-6, and TNF-α inflammatory mediators were assessed by ELISA test in the four experimental groups and also in the control group at different time intervals of 2, 24, and 48 hours and after 7 days. One-way analysis of variance (ANOVA) was used for statistical analysis of the obtained raw data followed by Tukey's post-hoc test for pair comparisons.

Results: the noncytotoxic concentration of green tea extract and Ledermix® paste equaled 12 µg/ml and 0.25 µg/ml, respectively. Time interval had no significant effect on the concentration of the mediators. Generally, both substances significantly decreased the concentration of IL-1β and IL-6 inflammatory mediators in comparison with the control group.

Conclusions: Green tea extract is less cytotoxic and more biocompatible than Ledermix® paste. Both Ledermix® paste and green tea extract expressed anti-inflammatory effects.

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