Volume 1, Issue 3 (7-2016)
J Res Dent Maxillofac Sci 2016, 1(3): 1-7 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Vatanpour M, Falah Doust A, Shojaee G, Jamshidian A, Farasat A. In Vitro Comparison of the Effect of Ledermix® Paste and Green Tea Extract on the Concentration of Inflammatory Mediators. J Res Dent Maxillofac Sci 2016; 1 (3) :1-7
URL: http://jrdms.dentaliau.ac.ir/article-1-110-en.html
URL: http://jrdms.dentaliau.ac.ir/article-1-110-en.html
1- Assistant Professor, Endodontics Dept, Dental Branch of Tehran, Islamic Azad University, Tehran, Iran
2- Post Graduate student, Endodontics Dept, Dental faculty,Qom university of medical sciences,Qom,Iran
3- Post Graduate student,Endodontics Dept, Dental Branch of Tehran, Islamic Azad University, Tehran, Iran. ,jmdn.ali@gmail.com
4- Pharmaceutical Sciences Branch,Islamic Azad University,Tehran,Iran
2- Post Graduate student, Endodontics Dept, Dental faculty,Qom university of medical sciences,Qom,Iran
3- Post Graduate student,Endodontics Dept, Dental Branch of Tehran, Islamic Azad University, Tehran, Iran. ,
4- Pharmaceutical Sciences Branch,Islamic Azad University,Tehran,Iran
Abstract: (6348 Views)
Background and aim: The purpose of this study is to compare the effect of Ledermix® paste and green tea extract on the concentration of inflammatory mediators.
Materials and methods: In this in-vitro experimental study, first, the noncytotoxic concentrations of Ledermix® medicament and green tea extract were determined by MTT assay. Afterwards, the concentrations of IL-1β, IL-6, and TNF-α inflammatory mediators were assessed by ELISA test in the four experimental groups and also in the control group at different time intervals of 2, 24, and 48 hours and after 7 days. One-way analysis of variance (ANOVA) was used for statistical analysis of the obtained raw data followed by Tukey's post-hoc test for pair comparisons.
Results: the noncytotoxic concentration of green tea extract and Ledermix® paste equaled 12 µg/ml and 0.25 µg/ml, respectively. Time interval had no significant effect on the concentration of the mediators. Generally, both substances significantly decreased the concentration of IL-1β and IL-6 inflammatory mediators in comparison with the control group.
Conclusions: Green tea extract is less cytotoxic and more biocompatible than Ledermix® paste. Both Ledermix® paste and green tea extract expressed anti-inflammatory effects.
Type of Study: Original article |
Subject:
Oral & maxillofacial surgery
References
1. Law A, Messer H. An evidence-based analysis of the antibacterial effectiveness of intracanal medicaments. J Endod 2004;30(10):689.94.
2. Silva TA, Garlet GP, Fukada SY, Silva JS, Cunha FQ. Chemokines in oral inflammatory diseases: apical periodontitis and periodontal disease. J Dent Res 2007;86(4):306-19.
3. Hauman CH, Love RM. Biocompatibility of dental materials used in contemporary therapy: a review. Part1. Intracanal drugs and substances. Int Endod J 2003;36(2):75-85.
4. Narotzki B, Rezinck AZ, Aizenbud D, Levy Y. Green Tea: A promising natural product in oral health. Arch Oral biol 2001;57(5);429-35.
5. Chng HK, Islam I, Yap AU, Tong YW, Koh ET. Properties of a new root-end filling material. J Endod 2005;31(9):665-8.
6. Nakanishi T, Mukai K, Yumoto H, Hirao K, Hosokawa Y, Matsuo T. Anti-inflammatory effect of catechin on cultured human dental pulp cells affected by bacteria-derived factors. Eur J Oral Sci 2010;118(2):145-50.
7. Bilmin k, Kopczynska B, Grib P. Influence of serum and albumin on the in vitro anandamide cytotoxicity toward C6golima cells assessed by the MTT cell viability assay: implications for the methodology of the MTT tests. Folia Neuropathol 2013;51(1);44-50.
8. Osorio RM, Hefti A, Vertucci FJ, Shawley AL. Cytotoxicity of endodontic materials. J Endod 1998;24(2):91-6.
9. Day PF, Duggal MS, High AS, Robertson A, Gregg TA, Ashley PF, et al. Discoloration of teeth after avulsion and replantation: results from a multicenter randomized controlled trial. J Endod 2011;37(8):1052-7.
10. Han DH, Jeong JH, Kim JH. Anti-proliferative and apoptosis induction activity of tea polyphenols on human promyelocytic Leukemia HL-60 cells. Antic ancer Res2009;29(4):1417-21.
11. EIdeniz AU, Mustafa K, Orstavik D, Dahl JE. Cytotoxicity of new resin-calcium hydroxide and silicone-based root canal sealers on fibroblasts derived from human gingiva and L929 cell lines. Int Endod J 2007;40(5):329-37.
12. Athansseadis B, Abottot PV, Wash LJ. The use of Calcium Hydroxide , antibiotics and biocides as antimicrobical medicaments in endodontics. Aust Dent J 2007;52(1 Suppl):S64-82.
13. McKay DL, Blumberg JB. The role of tea in human health: an update. J Am Coll Nutr 2002;21(1):1-13.
14. Cabrera C, Artacho R, Giménez R. Beneficial effects of green tea--a review. J Am Coll Nutrn 2006;25(2):79-99.
15. Taylor MA, Humen WR, Heithersay GS. Some effects of Ledermix paste and Pulpdent paste on mouse fibroblasts and on bacteria in vitro. Endod Dent Traumatol 1989;5(6):266-73.
16. Lee KM, Yeo M, Chouse JS, Jin JH, Park SJ , Cheong JY, et al. Protective mechanism of epigallocatechin-3-gallate against Helicobacter pylori-induced gastric epithelial cytotoxicity via the blockage of TLR-4 signaling. Helicobacter 2004;9(6):632-42.
17. Chen H, Teixeira FB, Ritter Al, Levin L, Trope M. The effect of intracanal anti-inflammatory medicaments on external root resorption of replanted dog teeth after extended extra-oral dry time. Dent Traumatol 2008;24(1):74-8.
18. Pezelj-Ribarić S, Magasić K, Prpić J, Miletić I, Karlović Z.Tumor necrosis factor-alpha in periapical tissue exudates of teeth with a periodontitis. Mediators Inflamm 2007;2007:69416.
19. Shimauchi H, Takayama S, Imai-Tanaka T, Okada H. Balance of interleukin-1 beta and interleukin-1 receptor antagonist in human periapical lesions. J Endod 1998;24(2):116-9.
20. Balto K, Sasaki H, Stashenko P. Interleukin-6 deficiency increases inflammatory bone destruction. Infect Immun 2001;69(2):744-50.
21. Thong YL, Messer HH, Siar CH, Saw LH. Periodontal response to two intracanal medicaments in replanted monkey incisors. Dent Traumatol 2001;17(6):254_9.
22. Lee YL, Hong CY, Kok SH, Hou KL, Lin YT, Chen MH, et al. An extract of green tea, epigallocatechin-3-gallate, reduces periapical lesions by inhibiting cysteine-rich61 expression in osteoblasts. J Endod 2009;35(2):206-11.
23. Hirao K, Yumoto H, Nakanishi T, Mukai K, Takahashi K, Takegawa D, et al. Tea catechins reduce inflammatory reactions via mitogen-activated protein Kinase pathways in toll-like receptor 2 ligand-stimulated dental pulp cells. Life Sci2010 24;86(17-18):654-60.
24. Vane JR. Antiinflammatory drugs and the many mediators of inflammation. Int J Tissue React 1987;9(1):1-14.
25. Jung IH, Yun JH, Cho AR, Kim CS, Chung WG, Choi SH. Effect of (-)-epigallocatechin-3-gallate on maintaining the periodontal ligament cell viability of avulsed teeth: a preliminary study. J Periodontal Implant Sci 2011;41(1):10-6.
26. Lenherr P, Allgayer N, Weiger R, Filippi A, Attin T, Krastl G. Tooth discoloration induced by endodontic materials: a laboratory study. Int Endod J2012;45(10):942-9.
27. Thomson AD, Athanassiadis B, Kahler B, Walsh L. Tooth discoloration: staining effects of various sealers and medicaments. Aust Endod J 2012;38(1):2-9.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
