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:: Volume 5, Issue 2 (Journal of Research in Dental & Maxillofacial Sciences Spring 2020) ::
J Res Dentomaxillofac Sci 2020, 5(2): 26-33 Back to browse issues page
Bone Repair via Osteon and Bio-Oss: A Comparative Histological and Histomorphometric Animal Study
F Sarkarat1, SA Vahdati2, HR Mahaseni aghdam * 3, Z Nematallahi4, M Farahmand5
1- Associated Prof, Department of Oral and Maxillofacial Surgery and Craniomaxillofacial Research Center, Dental Faculty, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2- Doctor of Dental Surgery, Advanced Specialty Education Programs in Endodontics and Implant Dentistry, Loma Linda University, School of Dentistry, Loma Linda, CA, USA
3- Assistant Prof, Department of Oral and Maxillofacial Surgery and Craniomaxillofacial Research Center, Dental Faculty, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. , hamidmahaseni@gmail.com
4- Doctor of Dental Surgery, Craniomaxillofacial Research Center, Dental Faculty, Tehran Medical Sciences, , Islamic Azad University, Tehran, Iran.
5- Postgraduate Student, Department of Orthodontics, Dental Faculty, Tehran Medical Sciences, , Islamic Azad University, Tehran, Iran
Abstract:   (243 Views)

Background and Aim: Autogenous bone grafts are considered the gold standard although they have several disadvantages, leading to a search for suitable alternative graft biomaterials. This study evaluates the histological and histomorphometric properties of regenerated bone in defects in rabbits following the application of two commercially available xenografts (Bio-Oss and Osteon).
Materials and Methods: This animal study was carried out on 14 New Zealand rabbit calvaria. Four 6.5-mm critical-size defect (CSD) models of bone regeneration were formed in each surgical site. The first defect was filled with Bio-Oss, the second with large Osteon (L-Osteon), the third with small Osteon (S-Osteon), and the last one remained unfilled (the control group). The cases were sacrificed. Bone forming properties (amount of new bone formation, inflammation, and foreign body reaction) were observed at 4- and 8-week intervals through histological and histomorphometric examinations. The Friedman test, Kruskal-Wallis test, and Wilcoxon test for multiple comparisons were used for data analysis. The level of statistical significance was set at 0.05.
Results: There was no statistically significant difference for regenerated bone among the four groups (P>0.05). The L-Osteon site showed more inflammation and foreign body reaction compared to the other groups. 
Conclusion: The results of this study showed that Bio-Oss and Osteon appear to be highly biocompatible and osteoconductive and can thus successfully be used as bone substitutes in augmentation procedures.

Keywords: Biocompatible Materials, Bio-Oss, Bone Grafting, Bone Formation, Bone Substitutes, Histology, Osteon
Full-Text [PDF 423 kb]   (50 Downloads) |   |   Full-Text (HTML)  (46 Views)  
Type of Study: Original article | Subject: Oral & maxillofacial surgery
* Corresponding Author Address: Islamic Azad University, Tehran, Iran
References
1. 1. Van Assche N, Michels S, Naert I, Quirynen M. Randomized controlled trial to compare two bone substitutes in the treatment of bony dehiscences. Clin Implant Dent Relat Res. 2013 Aug;15(4):558-68. [DOI:10.1111/j.1708-8208.2011.00408.x] [PMID]
2. Jensen OT, Shulman LB, Block MS, Iacono VJ. Report of the Sinus Consensus Conference of 1996. Int J Oral Maxillofac Implants. 1998;13 Suppl:11-45.
3. Sakkas A, Wilde F, Heufelder M, Winter K, Schramm A. Autogenous bone grafts in oral implantology-is it still a "gold standard"? A consecutive review of 279 patients with 456 clinical procedures. Int J Implant Dent. 2017 Dec;3(1):23. [DOI:10.1186/s40729-017-0084-4] [PMID] [PMCID]
4. Moy PK, Lundgren S, Holmes RE. Maxillary sinus augmentation: histomorphometric analysis of graft materials for maxillary sinus floor augmentation. J Oral Maxillofac Surg. 1993 Aug;51(8):857-62. [DOI:10.1016/S0278-2391(10)80103-X]
5. Pejrone G, Lorenzetti M, Mozzati M, Valente G, Schierano G. Sinus floor augmentation with autogenous iliac bone block grafts: a histological and histomorphometrical report on the two-step surgical technique. Int J Oral Maxillofac Surg. 2002;31(4):383-8. [DOI:10.1054/ijom.2002.0286] [PMID]
6. Kim YJ, Saiki CET, Silva K, Massuda CKM, de Souza Faloni AP, Braz-Silva PH, et al. Bone Formation in Grafts with Bio-Oss and Autogenous Bone at Different Proportions in Rabbit Calvaria. Int J Dent. 2020 Feb 19;2020:2494128. [DOI:10.1155/2020/2494128] [PMID] [PMCID]
7. Kurkcu M, Benlidayi ME, Cam B, Sertdemir Y. Anorganic bovine-derived hydroxyapatite vs β-tricalcium phosphate in sinus augmentation: a comparative histomorphometric study. J Oral Implantol. 2012 Sep;38 Spec No:519-26. [DOI:10.1563/AAID-JOI-D-11-00061] [PMID]
8. Simunek A, Kopecka D, Somanathan RV, Pilathadka S, Brazda T. Deproteinized bovine bone versus beta-tricalcium phosphate in sinus augmentation surgery: a comparative histologic and histomorphometric study. Int J Oral Maxillofac Implants. 2008 Sep-Oct;23(5):935-42.
9. Kim YK, Yun PY, Lim SC, Kim SG, Lee HJ, Ong JL. Clinical evaluations of OSTEON® as a new alloplastic material in sinus bone grafting and its effect on bone healing. J Biomed Mater Res B Appl Biomater. 2008;86(1):270-7. [DOI:10.1002/jbm.b.31015] [PMID]
10. Kim YK, Yun PY, Kim SG, Lim SC. Analysis of the healing process in sinus bone grafting using various grafting materials. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Feb;107(2):204-11. [DOI:10.1016/j.tripleo.2008.07.021] [PMID]
11. Bae JH, Kim YK, Kim SG, Yun PY, Kim JS. Sinus bone graft using new alloplastic bone graft material (Osteon)-II: clinical evaluation. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Mar;109(3):e14-20. [DOI:10.1016/j.tripleo.2009.10.047] [PMID]
12. Xuan F, Lee CU, Son JS, Jeong SM, Choi BH. A comparative study of the regenerative effect of sinus bone grafting with platelet-rich fibrin-mixed Bio-Oss® and commercial fibrin-mixed Bio-Oss®: an experimental study. J Craniomaxillofac Surg. 2014 Jun;42(4):e47-50. [DOI:10.1016/j.jcms.2013.05.029] [PMID]
13. Paknejad M, Rokn AR, Yaghobee S, Moradinejad P, Heidari M, Mehrfard A. Effects of two types of anorganic bovine bone on bone regeneration: a histological and histomorphometric study of rabbit calvaria. J Dent (Tehran). 2014 Nov;11(6):687-95.
14. Zhang X, Cai Q, Liu H, Heng B, Peng H, Song Y, et al. Osteoconductive effectiveness of bone graft derived from antler cancellous bone: an experimental study in the rabbit mandible defect model. Int J Oral Maxillofac Surg. 2012 Nov;41(11):1330-7. [DOI:10.1016/j.ijom.2012.05.014] [PMID]
15. Bodrumlu E, Muglali M, Sumer M, Guvenc T. The response of subcutaneous connective tissue to a new endodontic filling material. J Biomed Mater Res B Appl Biomater. 2008 Feb;84(2):463-7. [DOI:10.1002/jbm.b.30892] [PMID]
16. Anderson JM, Rodriguez A, Chang DT. Foreign body reaction to biomaterials. Semin Immunol. 2008 Apr;20(2):86-100. [DOI:10.1016/j.smim.2007.11.004] [PMID] [PMCID]
17. Dong J, Uemura T, Shirasaki Y, Tateishi T. Promotion of bone formation using highly pure porous beta-TCP combined with bone marrow-derived osteoprogenitor cells. Biomaterials. 2002 Dec;23(23):4493-502. [DOI:10.1016/S0142-9612(02)00193-X]
18. Poser L, Matthys R, Schawalder P, Pearce S, Alini M, Zeiter S. A standardized critical size defect model in normal and osteoporotic rats to evaluate bone tissue engineered constructs. Biomed Res Int. 2014;2014:348635. [DOI:10.1155/2014/348635] [PMID] [PMCID]
19. Busuttil Naudi K, Ayoub A, McMahon J, Di Silvio L, Lappin D, Hunter KD, et al. Mandibular reconstruction in the rabbit using beta-tricalcium phosphate (β-TCP) scaffolding and recombinant bone morphogenetic protein 7 (rhBMP-7) - histological, radiographic and mechanical evaluations. J Craniomaxillofac Surg. 2012 Dec;40(8):e461-9. [DOI:10.1016/j.jcms.2012.03.005] [PMID]
20. Hollinger JO, Kleinschmidt JC. The critical size defect as an experimental model to test bone repair materials. J Craniofac Surg. 1990 Jan;1(1):60-8. [DOI:10.1097/00001665-199001000-00011] [PMID]
21. Ren T, Ren J, Jia X, Pan K. The bone formation in vitro and mandibular defect repair using PLGA porous scaffolds. J Biomed Mater Res A. 2005 Sep 15;74(4):562-9. [DOI:10.1002/jbm.a.30324] [PMID]
22. Lu M, Rabie ABM. The effect of demineralized intramembranous bone matrix and basic fibroblast growth factor on the healing of allogeneic intramembranous bone grafts in the rabbit. Arch Oral Biol. 2002;47(12):831-41. [DOI:10.1016/S0003-9969(02)00119-X]
23. Mao T, Wang C, Zhang S, Wang H, Zhao M, Chen F, et al. An experimental study on rhBMP-2 composite bone substitute for repairing craniomaxillary bone defects. Chin J Dent Res. 1998 Dec;1(3):21-5.
24. Henkel KO, Gerber T, Dörfling P, Gundlach KK, Bienengräber V. Repair of bone defects by applying biomatrices with and without autologous osteoblasts. J Craniomaxillofac Surg. 2005 Feb;33(1):45-9. [DOI:10.1016/j.jcms.2004.08.005] [PMID]
25. Henkel KO, Gerber T, Lenz S, Gundlach KK, Bienengräber V. Macroscopical, histological, and morphometric studies of porous bone-replacement materials in minipigs 8 months after implantation. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Nov;102(5):606-13. [DOI:10.1016/j.tripleo.2005.10.034] [PMID]
26. Saadeh PB, Khosla RK, Mehrara BJ, Steinbrech DS, McCormick SA, DeVore DP, et al. Repair of a critical size defect in the rat mandible using allogenic type I collagen. J Craniofac Surg. 2001 Nov;12(6):573-9. [DOI:10.1097/00001665-200111000-00015] [PMID]
27. Arosarena OA, Collins WL. Defect repair in the rat mandible with bone morphogenic protein 5 and prostaglandin E1. Arch Otolaryngol Head Neck Surg. 2003 Oct;129(10):1125-30. [DOI:10.1001/archotol.129.10.1125] [PMID]
28. Pekkarinen T, Jämsä T, Määttä M, Hietala O, Jalovaara P. Reindeer BMP extract in the healing of critical-size bone defects in the radius of the rabbit. Acta Orthop. 2006 Dec;77(6):952-9. [DOI:10.1080/17453670610013286] [PMID]
29. Develioglu H, Unver Saraydin S, Kartal U. The bone-healing effect of a xenograft in a rat calvarial defect model. Dent Mater J. 2009 Jul;28(4):396-400. [DOI:10.4012/dmj.28.396] [PMID]
30. Mayfield LJ, Skoglund A, Hising P, Lang NP, Attström R. Evaluation following functional loading of titanium fixtures placed in ridges augmented by deproteinized bone mineral. A human case study. Clin Oral Implants Res. 2001 Oct;12(5):508-14. [DOI:10.1034/j.1600-0501.2001.120512.x] [PMID]
31. Artzi Z, Tal H, Dayan D. Porous bovine bone mineral in healing of human extraction sockets. Part 1: histomorphometric evaluations at 9 months. J Periodontol. 2000 Jun;71(6):1015-23. [DOI:10.1902/jop.2000.71.6.1015] [PMID]
32. Moghaddas O, Sarkarat F, Zarbakhsh A, Hoseini M, Bitaraf T. Effect of Ardox-X Active Oxygen-Containing Toothpaste on Periodontal Parameters in Dentate and Implant-Wearing Patients. J Res Dentomaxillofac Sci. 2019; 4 (4) :1-5. [DOI:10.29252/jrdms.4.4.1]
33. Piattelli M, Favero GA, Scarano A, Orsini G, Piattelli A. Bone reactions to anorganic bovine bone (Bio-Oss) used in sinus augmentation procedures: a histologic long-term report of 20 cases in humans. Int J Oral Maxillofac Implants. 1999 Nov-Dec;14(6):835-40.
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Sarkarat F, Vahdati S, Mahaseni aghdam H, Nematallahi Z, Farahmand M. Bone Repair via Osteon and Bio-Oss: A Comparative Histological and Histomorphometric Animal Study. J Res Dentomaxillofac Sci. 2020; 5 (2) :26-33
URL: http://jrdms.dentaliau.ac.ir/article-1-271-en.html


Volume 5, Issue 2 (Journal of Research in Dental & Maxillofacial Sciences Spring 2020) Back to browse issues page
Journal of Research in Dental and Maxillofacial Sciences
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