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:: Volume 4, Issue 2 (Journal of Research in Dental & Maxillofacial Sciences Spring 2019) ::
J Res Dentomaxillofac Sci 2019, 4(2): 7-11 Back to browse issues page
Effect of UV-Photofunctionalization on Bioactivity of Titanium to Promote Human Mesenchymal Stem Cells
MR Shbahangfar1, E Jafari2, A Tarashohi2, P Olyaee3, T Bitaraf * 4
1- Assistant professor, Periodontology Dept, Dental Faculty,Tehran Medical Sciences, Islamic Azad University of Medical Sciences, Tehran, Iran.
2- Dentist, Tehran, Iran.
3- Dentist, Frankfurt, Germany
4- Assistant professor, Dental Implant Research Center, Dental Faculty, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , taherehbitaraf@yahoo.com
Abstract:   (719 Views)
Background and Aim: The present study introduces photofunctionalization as a technique for tackling biological aging and increasing the bioactivity of titanium. This in-vitro study evaluated the effects of ultraviolet (UVC) light treatment of titanium surfaces with different time-related changes on the behavior and function of human mesenchymal stem cells (MSCs).
Materials and Methods: MSCs were cultured on nine untreated titanium surfaces (four-week-old surfaces; group 1), nine fresh UVC-treated surfaces (immediately after UV treatment; group 2), and nine three-day-old UVC-treated surfaces (group 3). Cellular proliferation and attachment were measured by MTT. Alkaline phosphatase (ALP) was assessed by total protein extraction, and the enzyme activity was evaluated using a special ALP kit. One-way analysis of variance (ANOVA) and post-hoc Tukey’s test were used to examine the effect of UVC on titanium surfaces.
Results: The mean attachment of MSCs to titanium disks in groups 1 to 3 was 0.118±0.003, 0.103±0.007, and 0.155±0.009, respectively. The mean proliferation of MSCs in groups 1 to 3 was 0.229±0.004, 0.189±0.023, and 0.298±0.020, respectively. The proliferation and attachment in group 3 were significantly higher compared to other groups (P<0.05). Speeds of MSCs growth in groups 1 to 3 were 94%, 81%, and 92%, respectively. The ALP activity of MSCs in groups 1 to 3 was 0.153±0.003, 0.187±0.003, and 0.161±0.003, respectively. The ALP activity in group 2 was significantly higher compared to other groups (P<0.05).
Conclusion: UVC pretreatment of titanium surfaces increases the ALP activity. However, cellular attachment and proliferation were not increased in the present study due to the high probability of laboratory error.
Keywords: Ultraviolet Rays, Cell Adhesion, Cell Proliferation, Mesenchymal Stem Cells, Photochemistry, Titanium, Radiation Effects
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Type of Study: Original article | Subject: Periodontology
* Corresponding Author Address: Dental Implant Research Center, Dental Faculty, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
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Shbahangfar M, Jafari E, Tarashohi A, Olyaee P, Bitaraf T. Effect of UV-Photofunctionalization on Bioactivity of Titanium to Promote Human Mesenchymal Stem Cells. J Res Dentomaxillofac Sci. 2019; 4 (2) :7-11
URL: http://jrdms.dentaliau.ac.ir/article-1-246-en.html

Volume 4, Issue 2 (Journal of Research in Dental & Maxillofacial Sciences Spring 2019) Back to browse issues page
Journal of Research in Dental and Maxillofacial Sciences
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