Volume 4, Issue 2 (6-2019)
J Res Dent Maxillofac Sci 2019, 4(2): 7-11 |
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Shbahangfar M, Jafari E, Tarashohi A, Olyaee P, Bitaraf T. Effect of UV-Photofunctionalization on Bioactivity of Titanium to Promote Human Mesenchymal Stem Cells. J Res Dent Maxillofac Sci 2019; 4 (2) :7-11
URL: http://jrdms.dentaliau.ac.ir/article-1-246-en.html
URL: http://jrdms.dentaliau.ac.ir/article-1-246-en.html
1- Assistant professor, Periodontology Dept, Dental Faculty,Tehran Medical Sciences
2- Dentist
3- Assistant professor, Dental Implant Research Center, Dental Faculty, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran ,taherehbitaraf@yahoo.com
2- Dentist
3- Assistant professor, Dental Implant Research Center, Dental Faculty, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran ,
Abstract: (3142 Views)
Background and Aim: The present study introduces photofunctionalization as a technique for tackling biological aging and increasing the bioactivity of titanium. This in-vitro study evaluated the effects of ultraviolet (UVC) light treatment of titanium surfaces with different time-related changes on the behavior and function of human mesenchymal stem cells (MSCs).
Materials and Methods: MSCs were cultured on nine untreated titanium surfaces (four-week-old surfaces; group 1), nine fresh UVC-treated surfaces (immediately after UV treatment; group 2), and nine three-day-old UVC-treated surfaces (group 3). Cellular proliferation and attachment were measured by MTT. Alkaline phosphatase (ALP) was assessed by total protein extraction, and the enzyme activity was evaluated using a special ALP kit. One-way analysis of variance (ANOVA) and post-hoc Tukey’s test were used to examine the effect of UVC on titanium surfaces.
Results: The mean attachment of MSCs to titanium disks in groups 1 to 3 was 0.118±0.003, 0.103±0.007, and 0.155±0.009, respectively. The mean proliferation of MSCs in groups 1 to 3 was 0.229±0.004, 0.189±0.023, and 0.298±0.020, respectively. The proliferation and attachment in group 3 were significantly higher compared to other groups (P<0.05). Speeds of MSCs growth in groups 1 to 3 were 94%, 81%, and 92%, respectively. The ALP activity of MSCs in groups 1 to 3 was 0.153±0.003, 0.187±0.003, and 0.161±0.003, respectively. The ALP activity in group 2 was significantly higher compared to other groups (P<0.05).
Conclusion: UVC pretreatment of titanium surfaces increases the ALP activity. However, cellular attachment and proliferation were not increased in the present study due to the high probability of laboratory error.
Materials and Methods: MSCs were cultured on nine untreated titanium surfaces (four-week-old surfaces; group 1), nine fresh UVC-treated surfaces (immediately after UV treatment; group 2), and nine three-day-old UVC-treated surfaces (group 3). Cellular proliferation and attachment were measured by MTT. Alkaline phosphatase (ALP) was assessed by total protein extraction, and the enzyme activity was evaluated using a special ALP kit. One-way analysis of variance (ANOVA) and post-hoc Tukey’s test were used to examine the effect of UVC on titanium surfaces.
Results: The mean attachment of MSCs to titanium disks in groups 1 to 3 was 0.118±0.003, 0.103±0.007, and 0.155±0.009, respectively. The mean proliferation of MSCs in groups 1 to 3 was 0.229±0.004, 0.189±0.023, and 0.298±0.020, respectively. The proliferation and attachment in group 3 were significantly higher compared to other groups (P<0.05). Speeds of MSCs growth in groups 1 to 3 were 94%, 81%, and 92%, respectively. The ALP activity of MSCs in groups 1 to 3 was 0.153±0.003, 0.187±0.003, and 0.161±0.003, respectively. The ALP activity in group 2 was significantly higher compared to other groups (P<0.05).
Conclusion: UVC pretreatment of titanium surfaces increases the ALP activity. However, cellular attachment and proliferation were not increased in the present study due to the high probability of laboratory error.
Keywords: Ultraviolet Rays, Cell Adhesion, Cell Proliferation, Mesenchymal Stem Cells, Photochemistry, Titanium, Radiation Effects
Type of Study: Original article |
Subject:
Periodontology
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