[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 4, Issue 4 (Journal of Research in Dental & Maxillofacial Sciences Autumn 2019) ::
J Res Dentomaxillofac Sci 2019, 4(4): 17-22 Back to browse issues page
Antimicrobial Activity of Aqueous Garlic Extract (Allium sativum) Against Porphyromonas gingivalis: An In-Vitro Study
S Alirezaei1, H Godarzi2, N Moezi ghadim * 3, A Maheri4
1- Assistant professor, Oral Medecine Dept, faculty of Dentistry,Tehran medical sciences, Islamic Azad University, Tehran, Iran
2- microbiology department medicin factuly-shahid beheshti university of medical sciences, tehran
3- Assistant professor, Oral Medecine Dept, Member ship of Dental Material Research Center, faculty of Dentistry,Tehran medical sciences, Islamic Azad University, Tehran, Iran , nahid_mz_g@yahoo.com
4- Dentist, Tehran, Iran
Abstract:   (112 Views)
Background and Aim: Oral hygiene is important against the development of chronic periodontitis. There are concerns about bacterial resistance to antibiotics. The current study aimed to determine the antimicrobial activity of aqueous garlic extract (Allium sativum) against Porphyromonas gingivalis (P. gingivalis).
Materials and Methods: Aqueous garlic extract was prepared, and the inhibitory effect of the extract was tested against P. gingivalis. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) against the control group (0.2% chlorhexidine) were also determined.
Results: Significant differences were observed concerning the MIC (1.21±0.37 µl) and MBC (1.44±0.67 µl) against P. gingivalis between the aqueous garlic extract and control groups (0.29±0.1 µl; P<0.001). There was a significant difference in the inhibitory zone against P. gingivalis between the aqueous garlic extract group (20.1±1.4 mm) and the control group (27.3±1.8 mm); the inhibitory zone was larger in the control group (P<0.000).
Conclusion: The results suggested that although chlorhexidine exhibited better antimicrobial activity against P. gingivalis, the aqueous garlic extract also showed acceptable results. Further research using different extraction methods and concentrations is suggested.
Keywords: Microbial Sensitivity Tests, Plant Extracts, Garlic, Porphyromonas gingivalis
Full-Text [PDF 255 kb]   (35 Downloads) |   |   Full-Text (HTML)  (21 Views)  
Type of Study: Original article | Subject: Oral pathology
* Corresponding Author Address: Islamic Azad University, Tehran, Iran
References
1. Shetty S, Thomas B, Shetty V, Bhandary R, Shetty RM. An in-vitro evaluation of the efficacy of garlic extract as an antimicrobial agent on periodontal pathogens: A microbiological study. Ayu. 2013 Oct;34(4):445-51. [DOI:10.4103/0974-8520.127732] [PMID] [PMCID]
2. Mageed MJ, Saliem Saif S. Antimicrobial effects of green tea extracts on Porphyromonas Gingivalis (in vitro study). J Dent Med Sci. 2015 Oct;14(10):33-9.
3. Ataee RA, Araqizade H, Yoosefi R, Mehrabi Tavana A, Ataee MH. Effect of Allium sativum Extract on Erythromycin and Methicillin Resistant Bacteria Isolated from Hospital Operating Room. J Med Bacteriol. 2016;5(1-2):7-14.
4. Saha SK, Saha S, Hossain MA, Paul SK. In vitro assessment of antibacterial effect of garlic (allium sativum) extracts on pseudomonas aeruginosa. Mymensingh Med J. 2015 Apr;24(2):222-32.
5. Gull I, Saeed M, Shaukat H, Aslam SM, Samra ZQ, Athar AM. Inhibitory effect of Allium sativum and Zingiber officinale extracts on clinically important drug resistant pathogenic bacteria. Ann Clin Microbiol Antimicrob. 2012 Apr 27;11:8. [DOI:10.1186/1476-0711-11-8] [PMID] [PMCID]
6. Viswanathan V, Phadatare AG, Mukne A. Antimycobacterial and Antibacterial Activity of Allium sativum Bulbs. Indian J Pharm Sci. 2014 May;76(3):256-61.
7. Bozcuk H, Ozdoğan M, Aykurt O, Topcuoğlu F, Ozturk H, Ekinci D, et al. Urginea maritima (L.) Baker (Liliaceae) extract induces more cytotoxicity than standard chemotherapeutics in the A549 non-small cell lung cancer (NSCLC) cell line. Turk J Med Sci. 2011;41(1):101-8.
8. Khaki A, Farnam A, Badie AD, Nikniaz H. Treatment Effects of Onion (Allium cepa) and Ginger (Zingiber officinale) on Sexual Behavior of Rat after Inducing an Antiepileptic Drug (lamotrigine). Balkan Med J. 2012 Sep;29(3):236-42. [DOI:10.5152/balkanmedj.2012.045] [PMID] [PMCID]
9. Asadpour R, Azari M, Hejazi M, Tayefi H, Zaboli N. Protective effects of garlic aquous extract (Allium sativum), vitamin E, and N-acetylcysteine on reproductive quality of male rats exposed to lead. Vet Res Forum. 2013 Autumn;4(4):251- 7.
10. Jabar MA, Al-Mossawi A. Susceptibility of some multiple resistant bacteria to garlic extract. Afr J Biotechnol. 2007;6(6):771 6.
11. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal. 2016 Apr;6(2):71-9. [DOI:10.1016/j.jpha.2015.11.005] [PMID] [PMCID]
12. Casella S, Leonardi M, Melai B, Fratini F, Pistelli L. The role of diallyl sulfides and dipropyl sulfides in the in vitro antimicrobial activity of the essential oil of garlic, Allium sativum L., and leek, Allium porrum L. Phytother Res. 2013 Mar;27(3):380-3. [DOI:10.1002/ptr.4725] [PMID]
13. Houshmand B, Mahjour F, Dianat O. Antibacterial effect of different concentrations of garlic (Allium sativum) extract on dental plaque bacteria. Indian J Dent Res. 2013 Jan-Feb;24(1):71-5. [DOI:10.4103/0970-9290.114957] [PMID]
14. Bakri IM, Douglas CW. Inhibitory effect of garlic extract on oral bacteria. Arch Oral Biol. 2005 Jul;50(7):645-51. [DOI:10.1016/j.archoralbio.2004.12.002] [PMID]
15. Groppo FC, Ramacciato JC, Simões RP, Flório FM, Sartoratto A. Antimicrobial activity of garlic, tea tree oil, and chlorhexidine against oral microorganisms. Int Dent J. 2002 Dec;52(6):433-7. [DOI:10.1111/j.1875-595X.2002.tb00638.x] [PMID]
16. Borlinghaus J, Albrecht F, Gruhlke MC, Nwachukwu ID, Slusarenko AJ. Allicin: chemistry and biological properties. Molecules. 2014 Aug 19;19(8):12591-618. [DOI:10.3390/molecules190812591] [PMID] [PMCID]
17. Mohamed AF, Elnima EI, Ghandour IA. Inhibitory Effect of Garlic Extract on Porphyromonas Gingivalis Isolated from Sudanese Aggressive Periodontitis Patients Compared to Chlorhexidine Digluconate 0.2%. J Chem Pharm Res. 2017;9(4):259-63
18. Listgarten MA, Lai CH. Unusual cell wall ultrastructure of Leptotrichia buccalis. J Bacteriol. 1975 Aug;123(2):747-9. [DOI:10.1128/JB.123.2.747-749.1975] [PMID] [PMCID]
19. Mozaffari Nejad AS, Shabani S, Bayat M, Hosseini SE. Antibacterial Effect of Garlic Aqueous Extract on Staphylococcus aureus in Hamburger. Jundishapur J Microbiol. 2014 Nov;7(11):e13134. [DOI:10.5812/jjm.13134] [PMID] [PMCID]
20. Karuppiah P, Rajaram S. Antibacterial effect of Allium sativum cloves and Zingiber officinale rhizomes against multiple-drug resistant clinical pathogens. Asian Pac J Trop Biomed. 2012 Aug;2(8):597-601. [DOI:10.1016/S2221-1691(12)60104-X]
21. Belguith H, Kthiri F, Chati A, Abu Sofah A, Ben Hamida A, Landoulsi A. Study of the effect of aqueous garlic extract (Allium sativum) on some Salmonella serovars isolates. Emir J Food Agric. 2010;22(3):189-206 [DOI:10.9755/ejfa.v22i3.4889]
22. Haji Fattahi F, Alirezaei S, Goodarzi H, Lesan L, Khalesi MA. Investigation of antifungal effect of aqueous extract of garlic on Candida albicans (INVITRO). J Res Dent Sci. 2019 Spring;16(1):20-6.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Alirezaei S, Godarzi H, Moezi ghadim N, Maheri A. Antimicrobial Activity of Aqueous Garlic Extract (Allium sativum) Against Porphyromonas gingivalis: An In-Vitro Study. J Res Dentomaxillofac Sci. 2019; 4 (4) :17-22
URL: http://jrdms.dentaliau.ac.ir/article-1-224-en.html


Volume 4, Issue 4 (Journal of Research in Dental & Maxillofacial Sciences Autumn 2019) Back to browse issues page
Journal of Research in Dental and Maxillofacial Sciences
Persian site map - English site map - Created in 0.05 seconds with 32 queries by YEKTAWEB 4030