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:: Volume 2, Issue 3 (Journal of Research in Dental & Maxillofacial Sciences summer 2017) ::
J Res Dentomaxillofac Sci 2017, 2(3): 10-15 Back to browse issues page
Evaluation of Micronucleus Frequency and Related Factors in the Buccal Mucosa of Cone Beam Computed Tomography-Exposed Subjects
S Farhadi1, M Jolehar2, K Babaeiha *3
1- Assistant professor, Oral & Maxillofacial pathology dept, Dental branch of Tehran, Islamic Azad University, Tehran, Iran, Islamic Azad University, Tehran, Iran.
2- Assistant professor, Oral & Maxillofacial pathology dept, Dental branch of Tehran, Islamic Azad University, Tehran, Iran., Islamic Azad University, Tehran, Iran
3- Dentist , kimibabaiha@yahoo.com
Abstract:   (1577 Views)
Background and aim: The ionizing radiation has been recognized to have deleterious effects on the DNA and induce cell death. Due to the importance of evaluating the DNA alterations in buccal mucosa cells induced by the ionizing radiation and by considering the limited volume of the samples used in previous studies, the present study aimed to assess the micronucleus (MN) incidence in the buccal mucosa of cone beam computed tomography (CBCT)-exposed patients of an oral and maxillofacial radiology center during 2016.
Materials and Methods: This descriptive study was conducted by using the target-based sampling. All the subjects, corresponding to the inclusion criteria, had been exposed to CBCT radiation according to their treatment plans. The specimens were scraped from the buccal mucosa of the participants by a damp spatula and were subsequently stained by Papanicolaou staining process. The percentage of the micronucleated cells was reported after evaluation under a light microscope. Also, the multiple linear regression (MLR) method was approached to statistically analyze the correlation between the MN frequency and the age and gender of the participants.
Results: The lowest and highest percentages of the MN frequency were respectively equal to 8.20% and 27.10% with the mean MN frequency of 17.68±4.98. Also, there was no significant correlation between the age and gender of the subjects and the MN frequency (P>0.05).
Conclusion: Based on the results of the present study, there was no significant correlation between the age and gender of the participants and the MN frequency.
Keywords: Micronucleus, Cytotoxicity, Cone Beam ComputedTomography
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Type of Study: Original article | Subject: Oral medicine
* Corresponding Author Address: Tehran, Iran
1. Kamboj M, Mahajan S. Micronucleus--an upcoming marker of genotoxic damage.Clin Oral Investig 2007;11(2):121-6.
2. Abbasi F, Farhadi S, Esmaili M. Efficacy of Pilocarpine and Bromhexin in improving radiotherapyinduced xerostomia. J Dent Res Dent Clin Dent Prospects 2013;7(2):86-90.
3. Parplys AC, Petermann E, Petersen C, Dikomey E, Borgmann K. DNA damage by X-rays and their impact on replication processes. Radiother Oncol. 2012;102(3):466-71.
4. Lorenzoni DC, Fracalossi AC, Carlin V, Ribeiro DA, Sant’Anna EF. Mutagenicity and cytotoxicity in patients submitted to ionizing radiation. Angle Orthod 2013;83(1):104-9.
5. Carlin V, Artioli AJ, Matsumoto MA, Borgo E, Oshima CT, Ribeiro DA. Biomonitoring of DNA damage and cytotoxicity in individuals exposed to cone beam computed tomography. Dentomaxillofac Radiol. 2010;39(5):295-9.
6. Stich HF, Rosin MP. Quantitating the synergistic effect of smoking and alcohol consumption with the micronucleus test on human buccal mucosal cells. Int J Cancer 1983:15;31(3):305-8.
7. Palaskar S , Jindal C. Evaluation Of Micronuclei Using Papanicolaou And May Grunwald Giemsa Stain In Individuals With Different Tobacco Habits – A Comparative Study. JCDR 2010;4(6):3607-13.
8. Stich HF, Rosin MP, Vallejera MO. Reduction with vitamin A and beta_caratene administration of proportion of micronucleated buccal mucosal cells in Asian betel nut and tobacco chewers. Lancet 1984;2;1(8388):1204-6.
9. Saeed H. Saeed , Wasen H. Younis. A cytopathological study of the effect of smoking on the oral epithelial cells in relation to oral health status by the micronucleus assay. J Bagh Coll Dentistry 2012;24(3):67-70.
10. Fenech M. The cytokinesis-block micronucleus technique and its application to genotoxicity studies in human populations. Environ Health Perspect 1993;101 Suppl 3:101-7.
11. Ishikawa H, Tian Y, Yamauchi T. Induction of micronuclei formation in preimplantation mouse embryos after maternal treatment with 2-bromopropane. Reprod Toxicol 2001;15(1):81-5.
12. Stich HF, Stich W, ParidaBB. Elevated frequency of micronucleated cells in the buccal mucosa of individuals at high risk for oral cancer: betel quid chewers. Cancer Lett 1982;17(2):125-34.
13. Majer BJ, Laky B, Knasmüller S, Kassie F. Use of the micronucleus assay with exfoliated epithelial cells as a biomarker for monitoring individuals at elevated risk of genetic damage and in chemoprevention trials. Mutat Res 2001;489(2-3):147-72.
14. Rosin MP, Dunn BP, Stich HF. Use of intermediate endpoints in Quantitating the response of precancerous lesions to chemopreventive agents. Can J Physiol Pharmacol 1987;65(3):483-7.
15. Naderi NJ , Farhadi S , Sarshar S . Micronucleus assay of buccal mucosa in smokers with the historyof smoking less and more than 10 years .Indian J Pathol Microbiol 2012;55(4):433-8.
16. Ribeiro DA. Cytogenetic biomonitoring in oral mucosa cells following dental X-ray. Dentomaxillofac Radiology 2012;41(3):181-4.
17. Holland N, Bolognesi C, Kirsch-Volders M, Bonassi S, Zeiger E, Knasmueller S, et al. The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: the HUMN project perspective on current status and knowledge gaps. Mutat Res 2008;659(1-2):93–108.
18. Farhadi S, Jahanbani J, Jariani A, Ghasemi S. Bio-Monitoring of the Nuclear Abnormalities in Smokers Using Buccal Exfoliated Cytology. Adv Biores 2016;7(4)128-33.
19. Farhadi S, Sadri D, Sarshar S. Micronucleus assay of Buccal mucosa: a Useful noninvasive approach in Screening of Genotoxic nuclear damage. Adv Biores 2016;7(4): 20-9.
20. Sarto F, Tomanin R, Giacomelli L, Canova A, Raimondi F, Ghiotto C, et al. Evaluation of chromosomal aberrations in lymphocytes and micronuclei in lymphocytes, oral mucosa and hair root cells of patients under antiblastic therapy. Mutat Res
21. 1990;228(2):157–69.
22. Neri M, Fucic A, Knudsen LE, Lando C, Merlo F, Bonassi S. Micronuclei frequency in children exposed to environmental mutagens: a review. Mutat Res 2003;544(2-3):243–54.
23. Cerqueira EM, Meireles JR, Lopes MA, Junqueira VC, Gomes-Filho IS, Trindade S, et al. Genotoxic effects of X-rays on keratinized mucosa cells during panoramic dental radiography. Dentomaxillofac Radiol 2008;37(7):398–403.
24. Popova L, Kishkilova D, Hadjidekova VB, Hristova RP, Atanasova P, Hadjidekova VV, et al. Micronucleus test in buccal epithelium cells from patients subjected to panoramic radiography. Dentomaxillofac Radiol 2007;36(3):168-71.
25. Ribeiro DA, Oliveira G, de Castro G, Angelieri F. Cytogenetic biomonitoring in patients exposed to dental X-rays: comparison between adults and children. Dentomaxillofac Radiol 2008;37(7):404–7.
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Farhadi S, Jolehar M, Babaeiha K. Evaluation of Micronucleus Frequency and Related Factors in the Buccal Mucosa of Cone Beam Computed Tomography-Exposed Subjects . J Res Dentomaxillofac Sci. 2017; 2 (3) :10-15
URL: http://jrdms.dentaliau.ac.ir/article-1-168-en.html

Volume 2, Issue 3 (Journal of Research in Dental & Maxillofacial Sciences summer 2017) Back to browse issues page
Journal of Research in Dental and Maxillofacial Sciences
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