Journal of Research in Dental
and Maxillofacial Sciences
Volume 11, Issue 2 (6-2026)
J Res Dent Maxillofac Sci 2026, 11(2): 148-155 |
Back to browse issues page
Ethics code: IR.IAU.DENTAL.REC.1403.088
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Khodabandehlou F, Zahedi Rad S, Alamdari Mahd M. Spectrophotometric Comparison of Color Change of White Spot Lesions after the Application of Two Types of Resin Infiltrants: An In Vitro Study. J Res Dent Maxillofac Sci 2026; 11 (2) :148-155
URL: http://jrdms.dentaliau.ac.ir/article-1-1330-en.html
URL: http://jrdms.dentaliau.ac.ir/article-1-1330-en.html
1- Dentist in private practice, Tehran, Iran
2- Department of Pediatric Dentistry, TeMS.C., Islamic Azad University, Tehran, Iran
3- Department of Pediatric Dentistry, TeMS.C., Islamic Azad University, Tehran, Iran ,mandanaalamdari@iau.ac.ir
2- Department of Pediatric Dentistry, TeMS.C., Islamic Azad University, Tehran, Iran
3- Department of Pediatric Dentistry, TeMS.C., Islamic Azad University, Tehran, Iran ,
Abstract: (4 Views)
Background and Aim: This study aimed to spectrophotometrically compare the color change (ΔE) of white spot lesions (WSLs) after the application of ICON and Cobalt resin infiltrants.
Materials and Methods: In this in vitro study, WSLs were artificially induced in 20 extracted sound human premolars by their 7-day immersion in a demineralizing solution with a pH of 4.4. The specimens were randomly assigned to two groups (n=10) for resin infiltration treatment with ICON and Cobalt resin infiltrants. The color parameters of the specimens were measured before the intervention (T0), after demineralization (T1), immediately after treatment (T2), and 4 weeks after treatment (T3) spectrophotometrically by using the CIEL*a*b* system. ΔE values were calculated and analyzed by independent t-test (alpha=0.05).
Results: In comparison of ΔE2-0, the difference was not statistically significant between ICON and Cobalt (P=0.234). In comparison of ΔE2-1, ICON (ΔE=12.78) showed a significantly better coverage than Cobalt (ΔE=7.25) (P=0.004). In comparison of ΔE3-2, ICON (ΔE =4.79) showed higher color stability than Cobalt (ΔE=5.24), but the difference was not significant (P=0.667).
Conclusion: The results of this study showed that both ICON and Cobalt effectively corrected the color of WSLs to match the color of sound enamel, although ICON showed a slightly superior performance. The two resin infiltrants also had a comparable color stability after 4 weeks.
Materials and Methods: In this in vitro study, WSLs were artificially induced in 20 extracted sound human premolars by their 7-day immersion in a demineralizing solution with a pH of 4.4. The specimens were randomly assigned to two groups (n=10) for resin infiltration treatment with ICON and Cobalt resin infiltrants. The color parameters of the specimens were measured before the intervention (T0), after demineralization (T1), immediately after treatment (T2), and 4 weeks after treatment (T3) spectrophotometrically by using the CIEL*a*b* system. ΔE values were calculated and analyzed by independent t-test (alpha=0.05).
Results: In comparison of ΔE2-0, the difference was not statistically significant between ICON and Cobalt (P=0.234). In comparison of ΔE2-1, ICON (ΔE=12.78) showed a significantly better coverage than Cobalt (ΔE=7.25) (P=0.004). In comparison of ΔE3-2, ICON (ΔE =4.79) showed higher color stability than Cobalt (ΔE=5.24), but the difference was not significant (P=0.667).
Conclusion: The results of this study showed that both ICON and Cobalt effectively corrected the color of WSLs to match the color of sound enamel, although ICON showed a slightly superior performance. The two resin infiltrants also had a comparable color stability after 4 weeks.
Type of Study: Original article |
Subject:
pediatric
References
1. Nagib T, Yassa M, Elheeny A. Spectrophotometric evaluation of color change of white spot lesions treated with three different approaches: an in vitro study. Egypt Dent J. 2023 Oct;69:2549-54. [DOI:10.21608/edj.2023.215807.2583]
2. Aref NS, Alrasheed MK. Casein phosphopeptide amorphous calcium phosphate and universal adhesive resin as a complementary approach for management of white spot lesions: an in-vitro study. Prog Orthod. 2022 Mar;23(1):10. [DOI:10.1186/s40510-022-00404-9] [PMID] [PMCID]
3. Sulieman M. An overview of tooth discoloration: extrinsic, intrinsic and internalized stains. Dent Update. 2005 Oct;32(8):463-4, 6-8, 71. [DOI:10.12968/denu.2005.32.8.463] [PMID]
4. Khoroushi M, Kachuie M. Prevention and treatment of white spot lesions in orthodontic patients. Contemp Clin Dent. 2017 Jan;8(1):11-9. [DOI:10.4103/ccd.ccd_216_17] [PMID] [PMCID]
5. Hammad SM, El-Wassefy NA, Alsayed MA. Evaluation of color changes of white spot lesions treated with three different treatment approaches: an in-vitro study. Dent Press J Orthod. 2020;25(1):26-7. [DOI:10.1590/2177-6709.25.1.27.e1-7.onl] [PMID] [PMCID]
6. Ballard RW, Hagan JL, Phaup AN, Sarkar N, Townsend JA, Armbruster PC. Evaluation of three commercially available materials for resolution of white spot lesions. Am J Orthod Dentofacial Orthop. 2013 Apr;143(4 Suppl):S78-84. [DOI:10.1016/j.ajodo.2012.08.020] [PMID]
7. Prasada KL, Penta PK, Ramya KM. Spectrophotometric evaluation of white spot lesion treatment using novel resin infiltration material (ICON®). J Conserv Dent. 2018 Sep;21(5):531-5. [DOI:10.4103/JCD.JCD_52_18] [PMID] [PMCID]
8. Dogra S, Goyal V, Gupta A, Joshi S, Kataria V, Saini J, et al. Spectrophotometric evaluation of color change in tooth enamel defects using resin infiltrate: an in vivo study. Int J Clin Pediatr Dent. 2020 Mar;13(2):150-4. [DOI:10.5005/jp-journals-10005-1727] [PMID] [PMCID]
9. Puleio F, Fiorillo L, Gorassini F, Iandolo A, Meto A, D'Amico C, et al. Systematic review on white spot lesions treatments. Eur J Dent. 2022 Feb;16(1):41-8. [DOI:10.1055/s-0041-1731931] [PMID] [PMCID]
10. Mohamed B, Marzouk ES, Zaher AR. Spectrophotometric evaluation of different protocols of resin infiltration of white spot lesions with various depths. Egypt Orthod J. 2017 Dec;52:31-44. [DOI:10.21608/eos.2017.78356]
11. Rafiee A, Memarpour M, Benam H. Evaluation of bleaching agent effects on color and microhardness change of silver diamine fluoride-treated demineralized primary tooth enamel: An in vitro study. BMC Oral Health. 2022 Aug;22(1):1-9. [DOI:10.1186/s12903-022-02371-3] [PMID] [PMCID]
12. Ozgul BM, Orhan K, Oz FT. Micro-computed tomographic analysis of progression of artificial enamel lesions in primary and permanent teeth after resin infiltration. J Oral Sci. 2015;57(3):177-83. [DOI:10.2334/josnusd.57.177] [PMID]
13. Hannig M, Hannig C. Nanobiomaterials in preventive dentistry. In: Subramani K, Ahmed W, editors. Nanobiomaterials in Clinical Dentistry. 2nd ed. Amsterdam: Elsevier; 2019. p. 201-2. [DOI:10.1016/B978-0-12-815886-9.00008-5]
14. Instructions for using the Cobalt Infiltrant Resin Kit [Internet]. Available from: https://cobalt-biomed.ir/ifu-resin-infiltrant/
15. Alexandria AK, Vieira TI, Pithon MM, da Silva Fidalgo TK, Fonseca-Gonçalves A, Valença AMG, et al. In vitro enamel erosion and abrasion-inhibiting effect of different fluoride varnishes. Arch Oral Biol. 2017 May;77:39-43. [DOI:10.1016/j.archoralbio.2017.01.010] [PMID]
16. Gousalya V, Prabu D, Dhamodhar D, Bharathwaj VV, Sindhu R, Elakiya S. Systematic review on the efficacy of Icon resin infiltration on white spot lesions. Indian J Contemp Dent. 2023;11(1):18-24. [DOI:10.37506/ijocd.v11i1.18813]
17. Zaazou MH, Saleh RS, Hassan SN, Abdelnabi A, Zaki ZM, Hamdy TM, et al. Effectiveness of low-viscosity resin infiltration (Icon) on color change of enamel white spot lesions: 1-year follow-up clinical study. Bull Natl Res Cent. 2024 Jun;48(1):62. [DOI:10.1186/s42269-024-01219-w]
18. Schoppmeier CM, von Kohout M, Sun L, Janson M, Deeg I, Barbe AG, et al. Influence of home or in-office tooth bleaching on the color stability of white-spot lesions after resin infiltration: An in vitro comparison. J Am Dent Assoc D. 2025 Jul;156(7):517-29. [DOI:10.1016/j.adaj.2025.04.009] [PMID]
19. Al-Saeed EJ, AlMarhoon ZW, Al-Eid ZA, AlAhmari TA, AlJamed SH, AlSarhan R, et al. Properties, success, and applications of resin infiltration for minimal invasive restoration: A scoping review. Arch Pharm Pract. 2022;13(2-2022):110-5. [DOI:10.51847/nABHjPaqO7]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |