Journal of Research in Dental
and Maxillofacial Sciences
Volume 11, Issue 2 (6-2026)
J Res Dent Maxillofac Sci 2026, 11(2): 164-173 |
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Ethics code: IR.IAU.DENTAL.REC.1403.026
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Ebrahimgol S, Nemati Anaraki S, Ghanaat M, Ramouz E, Hoorizad ganjkar M. Effect of a Hemostatic Agent on Bond Strength and Durability of Bonding in Three Different Adhesive Systems. J Res Dent Maxillofac Sci 2026; 11 (2) :164-173
URL: http://jrdms.dentaliau.ac.ir/article-1-1226-en.html
URL: http://jrdms.dentaliau.ac.ir/article-1-1226-en.html
Saeedeh Ebrahimgol1 
, Saeed Nemati Anaraki1 , Mahsa Ghanaat1 , Emad Ramouz1 , Maryam Hoorizad ganjkar *2
, Saeed Nemati Anaraki1 , Mahsa Ghanaat1 , Emad Ramouz1 , Maryam Hoorizad ganjkar *2
1- Department of Operative Dentistry, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2- Department of Operative Dentistry, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran ,mahoorizad@yahoo.com
2- Department of Operative Dentistry, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran ,
Abstract: (12 Views)
Background and Aim: Hemostatic agents may affect the hybrid layer quality. This study evaluated the effect of a hemostatic agent on microtensile bond strength (μTBS) and bonding durability in three adhesive systems.
Materials and Methods: In this in vitro study, 120 extracted human molars were randomly divided into two main groups (n=60) based on aging conditions: (I) 24-hour storage in distilled water and (II) thermocycling (10,000 cycles, 5°C-55°C). Each group was further subdivided into 6 subgroups (n=10) according to the use of Viscostat Clear and the adhesive system: Adper Single Bond, Clearfil SE Bond, and All-Bond Universal. Composite resin (Filtek Z250) was applied, and μTBS was measured with a testing machine. Failure modes were observed under an optical microscope, and resin-dentin interface was examined by scanning electron microscopy (SEM). Data were analyzed using three-way fixed-effects ANOVA and Tukey’s post-hoc test (α=0.05).
Results: The highest μTBS was found in the Adper Single Bond group without hemostatic agent after 24 hours of storage (17.37±5.43 MPa), and the lowest was in the All-Bond Universal group with hemostatic agent after thermocycling (5.44±1.21 MPa). At 24 hours, no significant difference existed between contaminated and uncontaminated groups (P>0.05). After thermocycling, Adper Single Bond showed a significant reduction in bond strength when exposed to Viscostat Clear (P=0.000). Adhesive failure predominated in all groups.
Conclusion: The adhesive type and aging process significantly affected the μTBS. While Viscostat Clear did not significantly affect the 24-hour μTBS, it reduced bonding durability after thermocycling in Adper Single Bond group.
Materials and Methods: In this in vitro study, 120 extracted human molars were randomly divided into two main groups (n=60) based on aging conditions: (I) 24-hour storage in distilled water and (II) thermocycling (10,000 cycles, 5°C-55°C). Each group was further subdivided into 6 subgroups (n=10) according to the use of Viscostat Clear and the adhesive system: Adper Single Bond, Clearfil SE Bond, and All-Bond Universal. Composite resin (Filtek Z250) was applied, and μTBS was measured with a testing machine. Failure modes were observed under an optical microscope, and resin-dentin interface was examined by scanning electron microscopy (SEM). Data were analyzed using three-way fixed-effects ANOVA and Tukey’s post-hoc test (α=0.05).
Results: The highest μTBS was found in the Adper Single Bond group without hemostatic agent after 24 hours of storage (17.37±5.43 MPa), and the lowest was in the All-Bond Universal group with hemostatic agent after thermocycling (5.44±1.21 MPa). At 24 hours, no significant difference existed between contaminated and uncontaminated groups (P>0.05). After thermocycling, Adper Single Bond showed a significant reduction in bond strength when exposed to Viscostat Clear (P=0.000). Adhesive failure predominated in all groups.
Conclusion: The adhesive type and aging process significantly affected the μTBS. While Viscostat Clear did not significantly affect the 24-hour μTBS, it reduced bonding durability after thermocycling in Adper Single Bond group.
Type of Study: Original article |
Subject:
Restorative Dentistry
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