Volume 10, Issue 2 (6-2025)
J Res Dent Maxillofac Sci 2025, 10(2): 103-110 |
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Ethics code: IR.IAU.DENTAL.REC.1401.082
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Mohitmafi N, Parsania M, Alaee A, Olyaee P, Vahedi M. In Vitro Effect of Photodynamic Therapy with 660 nm Diode Laser and Methylene Blue Photosensitizer on Acyclovir-Resistant Herpes Simplex Virus Type 1. J Res Dent Maxillofac Sci 2025; 10 (2) :103-110
URL: http://jrdms.dentaliau.ac.ir/article-1-800-en.html
URL: http://jrdms.dentaliau.ac.ir/article-1-800-en.html
1- Department of Oral and Maxillofacial Medicine, TeMS.C., Islamic Azad University, Tehran, Iran.
2- Department of Microbiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
3- Department of Oral Medicine, Dental Material Research Center, TeMS.C., Islamic Azad University, Tehran, Iran.
4- Department of Orthodontics, Urmia University of Medical Science, School of Dentistry, Urmia, Iran.
5- Department of Oral and Maxillofacial Medicine, TeMS.C., Islamic Azad University, Tehran, Iran. ,vahedi_md@yahoo.com
2- Department of Microbiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
3- Department of Oral Medicine, Dental Material Research Center, TeMS.C., Islamic Azad University, Tehran, Iran.
4- Department of Orthodontics, Urmia University of Medical Science, School of Dentistry, Urmia, Iran.
5- Department of Oral and Maxillofacial Medicine, TeMS.C., Islamic Azad University, Tehran, Iran. ,
Abstract: (57 Views)
Background and Aim: Herpes simplex virus type 1 (HSV-1) is one of the most common infections of the mouth and face. This study was conducted to assess the effect of photodynamic therapy (PDT) with 660 nm diode laser and methylene blue photosensitizer (PS) with different concentrations and radiation parameters on acyclovir-resistant HSV-1.
Materials and Methods: In this in vitro study, first the cytotoxicity of methylene blue for HeLa cells was assessed by the methyl thiazolyl tetrazolium (MTT) assay, and its highest non-toxic concentrations were found (0.001%, 0.002%, 0.005%, and 0.01%). Laser was used with 660 nm wavelength, 100 mW power, and 10, 20, and 30 J/cm2 energy densities for 50, 100, and 150 seconds. The samples were divided into 20 groups, including 12 PDT groups with laser and methylene blue, 3 groups subjected to laser irradiation alone, 4 groups exposed to methylene blue alone with different concentrations, and one negative control group. After laser irradiation, HSV-1 was titrated by the tissue culture infectious dose 50 (TCID50) method. Two-way ANOVA was used for data analysis (alpha=0.05).
Results: The effects of methylene blue concentration (0.001%, 0.002%, 0.005%, and 0.01%) (P=0.675) and laser energy density (10, 20, 30 J/cm2) (P=0.914), and their interaction effect (P=0.977) on the titer of acyclovir-resistant HSV-1 were not significant.
Conclusion: PDT with 660 nm laser with different energy densities and different methylene blue concentrations had no significant effect on acyclovir-resistant HSV-1.
Materials and Methods: In this in vitro study, first the cytotoxicity of methylene blue for HeLa cells was assessed by the methyl thiazolyl tetrazolium (MTT) assay, and its highest non-toxic concentrations were found (0.001%, 0.002%, 0.005%, and 0.01%). Laser was used with 660 nm wavelength, 100 mW power, and 10, 20, and 30 J/cm2 energy densities for 50, 100, and 150 seconds. The samples were divided into 20 groups, including 12 PDT groups with laser and methylene blue, 3 groups subjected to laser irradiation alone, 4 groups exposed to methylene blue alone with different concentrations, and one negative control group. After laser irradiation, HSV-1 was titrated by the tissue culture infectious dose 50 (TCID50) method. Two-way ANOVA was used for data analysis (alpha=0.05).
Results: The effects of methylene blue concentration (0.001%, 0.002%, 0.005%, and 0.01%) (P=0.675) and laser energy density (10, 20, 30 J/cm2) (P=0.914), and their interaction effect (P=0.977) on the titer of acyclovir-resistant HSV-1 were not significant.
Conclusion: PDT with 660 nm laser with different energy densities and different methylene blue concentrations had no significant effect on acyclovir-resistant HSV-1.
Type of Study: Original article |
Subject:
Oral medicine
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