Volume 10, Issue 2 (6-2025)                   J Res Dent Maxillofac Sci 2025, 10(2): 103-110 | Back to browse issues page

Ethics code: IR.IAU.DENTAL.REC.1401.082

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mohitmafi N, Parsania M, Alaee A, Olyaee P, Vahedi M. In Vitro Effect of Photodynamic Therapy with 660 nm Diode Laser and Methylene Blue Photosensitizer on Acyclovir-Resistant Herpes Simplex Virus Type 1. J Res Dent Maxillofac Sci 2025; 10 (2) :103-110
URL: http://jrdms.dentaliau.ac.ir/article-1-800-en.html
1- Department of Oral and Maxillofacial Medicine, TeMS.C., Islamic Azad University, Tehran, Iran.
2- Department of Microbiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
3- Department of Oral Medicine, Dental Material Research Center, TeMS.C., Islamic Azad University, Tehran, Iran.
4- Department of Orthodontics, Urmia University of Medical Science, School of Dentistry, Urmia, Iran.
5- Department of Oral and Maxillofacial Medicine, TeMS.C., Islamic Azad University, Tehran, Iran. , vahedi_md@yahoo.com
Abstract:   (56 Views)
Background and Aim: Herpes simplex virus type 1 (HSV-1) is one of the most common infections of the mouth and face. This study was conducted to assess the effect of photodynamic therapy (PDT) with 660 nm diode laser and methylene blue photosensitizer (PS) with different concentrations and radiation parameters on acyclovir-resistant HSV-1.   
Materials and Methods: In this in vitro study, first the cytotoxicity of methylene blue for HeLa cells was assessed by the methyl thiazolyl tetrazolium (MTT) assay, and its highest non-toxic concentrations were found (0.001%, 0.002%, 0.005%, and 0.01%). Laser was used with 660 nm wavelength, 100 mW power, and 10, 20, and 30 J/cm2 energy densities for 50, 100, and 150 seconds. The samples were divided into 20 groups, including 12 PDT groups with laser and methylene blue, 3 groups subjected to laser irradiation alone, 4 groups exposed to methylene blue alone with different concentrations, and one negative control group. After laser irradiation, HSV-1 was titrated by the tissue culture infectious dose 50 (TCID50) method. Two-way ANOVA was used for data analysis (alpha=0.05).   
Results: The effects of methylene blue concentration (0.001%, 0.002%, 0.005%, and 0.01%) (P=0.675) and laser energy density (10, 20, 30 J/cm2) (P=0.914), and their interaction effect (P=0.977) on the titer of acyclovir-resistant HSV-1 were not significant.
Conclusion: PDT with 660 nm laser with different energy densities and different methylene blue concentrations had no significant effect on acyclovir-resistant HSV-1.
 
Full-Text [PDF 418 kb]   (23 Downloads)    
Type of Study: Original article | Subject: Oral medicine

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2025 CC BY-NC 4.0 | Journal of Research in Dental and Maxillofacial Sciences

Designed & Developed by: Yektaweb