Volume 10, Issue 2 (6-2025)
J Res Dent Maxillofac Sci 2025, 10(2): 82-87 |
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Ethics code: IR.IAU.DENTAL.REC.1399.299
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Fazlyab M, Hosseini Z S, Vatanpour M, Rezaei F. Effect of 660 nm and 808 nm Low-Level Diode Laser Irradiation on Production of Vascular Endothelial Growth Factor by Human Gingival Fibroblasts: An in vitro Study. J Res Dent Maxillofac Sci 2025; 10 (2) :82-87
URL: http://jrdms.dentaliau.ac.ir/article-1-797-en.html
URL: http://jrdms.dentaliau.ac.ir/article-1-797-en.html
1- Department of Endodontics, TeMS.C., Islamic Azad University, Tehran, Iran
2- Private Dentist, Tehran, Iran.
3- Department of Endodontics, TeMS.C., Islamic Azad University, Tehran, Iran ,dr.fatemeh.rezaeii@gmail.com
2- Private Dentist, Tehran, Iran.
3- Department of Endodontics, TeMS.C., Islamic Azad University, Tehran, Iran ,
Abstract: (300 Views)
Background and Aim: This study aimed to assess the effect of 660 nm and 808 nm low-level diode laser irradiation on the production of vascular endothelial growth factor (VEGF) by human gingival fibroblasts (HGFs).
Materials and Methods: In this in vitro experimental study, HGFs were cultured in McCoy’s 5A modified medium and assigned to three groups of control, 660 nm laser irradiation, and 808 nm laser irradiation. The concentration of VEGF was measured in the three groups at 1, 3, and 7 days after the intervention using ELISA. The three groups were compared in this respect using repeated measures ANOVA. Statistical significance was defined as P<0.05.
Results: The concentration of VEGF in 808 nm laser group was significantly higher than that in the control and 660 nm laser groups (P<0.05). Also, the concentration of VEGF in 660 nm laser group was significantly higher than that in the control group (P<0.05). The concentration of VEGF at 7 days was significantly higher than that at 1 and 3 days, and the value at 3 days was significantly higher than that at 1 day in all three groups (P<0.05).
Conclusion: Within the limitations of this study, it appears that irradiation of 660 and 808 nm diode laser can increase the production of VEGF by HGFs.
Materials and Methods: In this in vitro experimental study, HGFs were cultured in McCoy’s 5A modified medium and assigned to three groups of control, 660 nm laser irradiation, and 808 nm laser irradiation. The concentration of VEGF was measured in the three groups at 1, 3, and 7 days after the intervention using ELISA. The three groups were compared in this respect using repeated measures ANOVA. Statistical significance was defined as P<0.05.
Results: The concentration of VEGF in 808 nm laser group was significantly higher than that in the control and 660 nm laser groups (P<0.05). Also, the concentration of VEGF in 660 nm laser group was significantly higher than that in the control group (P<0.05). The concentration of VEGF at 7 days was significantly higher than that at 1 and 3 days, and the value at 3 days was significantly higher than that at 1 day in all three groups (P<0.05).
Conclusion: Within the limitations of this study, it appears that irradiation of 660 and 808 nm diode laser can increase the production of VEGF by HGFs.
Type of Study: Original article |
Subject:
Endodontics
References
1. Raedel M, Hartmann A, Bohm S, Walter MH. Three-year outcomes of root canal treatment: Mining an insurance database. J Dent. 2015 Apr;43(4):412-7. [DOI:10.1016/j.jdent.2015.01.013]
2. Bakhtiar H, Mazidi S A, Mohammadi Asl S, Ellini MR, Moshiri A, Nekoofar MH, Dummer PMH. The role of stem cell therapy in regeneration of dentine-pulp complex: a systematic review. Prog Biomater. 2018 Dec;7(4):249-68. [DOI:10.1007/s40204-018-0100-7] [PMID]
3. Soares PV, Santos-Filho PCF, Queiroz EC, Araújo TC, Campos RE, Araújo CA, Soares CJ. Fracture resistance and stress distribution in endodontically treated maxillary premolars restored with composite resin. J Prosthodont. 2008 Feb;17(2):114-9. [DOI:10.1111/j.1532-849X.2007.00258.x] [PMID]
4. Galler KM, D'Souza RN, Federlin M, Cavender AC, Hartgerink JD, Hecker S, Schmalz G. Dentin conditioning codetermines cell fate in regenerative endodontics. J Endod. 2011 Nov;37(11):1536-41. [DOI:10.1016/j.joen.2011.08.027] [PMID]
5. Martignago CC, Oliveira RF, Pires-Oliveira DA, Oliveira PD, Pacheco Soares C, Monzani PS, Poli-Frederico RC. Effect of low-level laser therapy on the gene expression of collagen and vascular endothelial growth factor in a culture of fibroblast cells in mice. Lasers Med Sci. 2015 Jan;30(1):203-8. [DOI:10.1007/s10103-014-1644-y]
6. Saghiri MA, Asatourian A, Sorenson CM, Sheibani N. Role of angiogenesis in endodontics: contributions of stem cells and proangiogenic and antiangiogenic factors to dental pulp regeneration. J Endod. 2015 Jun;41(6):797-803. [DOI:10.1016/j.joen.2014.12.019] [PMID] []
7. Ferrara N. Vascular endothelial growth factor. Eur J Cancer. 1996 Dec;32A(14):2413-22. [DOI:10.1016/S0959-8049(96)00387-5] [PMID]
8. Laschke MW, Harder Y, Amon M, Martin I, Farhadi J, Ring A, et al. Angiogenesis in tissue engineering: breathing life into constructed tissue substitutes. Tissue Eng. 2006 Aug;12(8):2093-104. [DOI:10.1089/ten.2006.12.2093] [PMID]
9. Malhotra N. Induced Pluripotent Stem (iPS) Cells in Dentistry: A Review. Int J Stem Cells. 2016 Nov 30;9(2):176-85. [DOI:10.15283/ijsc16029] [PMID] []
10. El Nawam H, El Backly R, Zaky A, Abdallah A. Low-level laser therapy affects dentinogenesis and angiogenesis of in vitro 3D cultures of dentin-pulp complex. Lasers Med Sci. 2019 Oct;34(8):1689-98. [DOI:10.1007/s10103-019-02804-6] [PMID]
11. Arany PR, Cho A, Hunt TD, Sidhu G, Shin K, Hahm E, et al. Photoactivation of endogenous latent transforming growth factor-β1 directs dental stem cell differentiation for regeneration. Sci Transl Med. 2014 May 28;6(238):238ra69. [DOI:10.1126/scitranslmed.3008234] [PMID]
12. de Oliveira TS, Serra AJ, Manchini MT, Bassaneze V, Krieger JE, de Tarso Camillo de Carvalho Pet al. Effects of low level laser therapy on attachment, proliferation, and gene expression of VEGF and VEGF receptor 2 of adipocyte-derived mesenchymal stem cells cultivated under nutritional deficiency. Lasers Med Sci. 2015 Jan;30(1):217-23. [DOI:10.1007/s10103-014-1646-9] [PMID]
13. Gasparyan L, Brill GE, Makela A. Activation of angiogenesis under influence of red low level laser radiation. Proc SPIE. 2005;5968:45-50. [DOI:10.1117/12.660039]
14. Pansani TN, Basso FG, Turrioni AP, Soares DG, Hebling J, de Souza Costa CA. Effects of low-level laser therapy and epidermal growth factor on the activities of gingival fibroblasts obtained from young or elderly individuals. Lasers Med Sci. 2017 Jan;32(1):45-52. [DOI:10.1007/s10103-016-2081-x] [PMID]
15. Eslami H, Motahari P, Safari E, Seyyedi M. Evaluation effect of low level Helium-Neon laser and Iranian propolis extract on Collagen Type I gene expression by human gingival fibroblasts: an in vitro study. Laser Ther. 2017 Jun 30;26(2):105-12. [DOI:10.5978/islsm.17-OR-8] [PMID] []
16. Bloise N, Ceccarelli G, Minzioni P, Vercellino M, Benedetti L, De Angelis MG, Imbriani M, Visai L. Investigation of low-level laser therapy potentiality on proliferation and differentiation of human osteoblast-like cells in the absence/presence of osteogenic factors. J Biomed Opt. 2013 Dec;18(12):128006. [DOI:10.1117/1.JBO.18.12.128006] [PMID]
17. Szezerbaty SKF, de Oliveira RF, Pires-Oliveira DAA, Soares CP, Sartori D, Poli-Frederico RC. The effect of low-level laser therapy (660 nm) on the gene expression involved in tissue repair. Lasers Med Sci. 2018 Feb;33(2):315-21. [DOI:10.1007/s10103-017-2375-7]
18. Chiarotto GB, Neves LM, Esquisatto MA, do Amaral ME, dos Santos GM, Mendonça FA. Effects of laser irradiation (670-nm InGaP and 830-nm GaAlAs) on burn of second-degree in rats. Lasers Med Sci. 2014 Sep;29(5):1685-93. [DOI:10.1007/s10103-014-1573-9] [PMID]
19. Illescas-Montes R, Melguizo-Rodríguez L, García-Martínez O, de Luna-Bertos E, Manzano-Moreno FJ, Ruiz C, Ramos-Torrecillas J. Human Fibroblast Gene Expression Modulation Using 940 NM Diode Laser. Sci Rep. 2019 Aug 19;9(1):12037. [DOI:10.1038/s41598-019-48595-2] [PMID]
20. Szymczyszyn A, Doroszko A, Szahidewicz-Krupska E, Rola P, Gutherc R, Jasiczek J, Mazur G, Derkacz A. Effect of the transdermal low-level laser therapy on endothelial function. Lasers Med Sci. 2016 Sep;31(7):1301-7. [DOI:10.1007/s10103-016-1971-2] [PMID]
21. Cury V, Moretti AI, Assis L, Bossini P, Crusca Jde S, Neto CB, et al. Low level laser therapy increases angiogenesis in a model of ischemic skin flap in rats mediated by VEGF, HIF-1α and MMP-2. J Photochem Photobiol B. 2013 Aug 5;125:164-70. [DOI:10.1016/j.jphotobiol.2013.06.004] [PMID]
22. Vitor LLR, Bergamo MTOP, Lourenço-Neto N, Sakai VT, Oliveira RC, Cruvinel T, et al. Photobiomodulation effect on angiogenic proteins produced and released by dental pulp cells. Clin Oral Investig. 2020 Dec;24(12):4343-54. [DOI:10.1007/s00784-020-03298-1] [PMID]
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