Volume 9, Issue 4 (12-2024)
J Res Dent Maxillofac Sci 2024, 9(4): 250-256 |
Back to browse issues page
Ethics code: IR.TBZMED.REC.1399.621
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Rezaei Y, Ahmad Pour Zabarjad A, Katebi K, Bagheri Maleki S. Effect of Chlorhexidine Application on Microleakage of Pit and Fissure Sealant Therapy with Self-Etch and Etch-And-Rinse Adhesives: An in Vitro Study. J Res Dent Maxillofac Sci 2024; 9 (4) :250-256
URL: http://jrdms.dentaliau.ac.ir/article-1-592-en.html
URL: http://jrdms.dentaliau.ac.ir/article-1-592-en.html
1- Department of Dental Biomaterials, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran
2- Private Dental Practice, Tabriz, Iran
3- Department of Oral and Maxillofacial Medicine, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran ,k_katebi@yahoo.co.uk
2- Private Dental Practice, Tabriz, Iran
3- Department of Oral and Maxillofacial Medicine, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran ,
Abstract: (107 Views)
Background and Aim: Application of disinfectants in pits and fissures is a commonly used method to lower the cariogenic bacterial count in teeth. This study compared the effect of applying chlorhexidine on microleakage of pit and fissure sealant therapy using two adhesive systems.
Materials and Methods: In this in vitro study, 60 extracted molar teeth were randomly assigned to four groups (n=15): control group 1 (etch-and-rinse adhesive), control group 2 (self-etch adhesive), group 3 (etch-and-rinse adhesive and 0.2% chlorhexidine), and group 4 (self-etch adhesive and 0.2% chlorhexidine). After applying the sealant and subjecting the teeth to thermocycling, the teeth were immersed in 5% methylene blue solution. The teeth were sectioned into four parts and the dye penetration depth was measured under a stereomicroscope. Data were analyzed by the Mann–Whitney and Kruskal–Wallis tests using SPSS 24 (alpha=0.05).
Results: Microleakage was significantly lower in the self-etch adhesive system compared to the etch-and-rinse system in the control groups (P<0.05). For the etch-and-rinse adhesive, there was no significant difference in microleakage between the two groups with chlorhexidine (1.67±1.11) and without chlorhexidine (1.33±0.81) (P>0.05). Similarly, in the self-etch adhesive, microleakage did not significantly differ between groups with chlorhexidine (1.07±0.45) and without chlorhexidine (1.07±0.70) (P>0.05). The interaction effect of adhesive type and chlorhexidine on microleakage was not significant (P=0.428).
Conclusion: Application of chlorhexidine with both self-etch and etch-and-rinse adhesive systems did not significantly affect the microleakage of fissure sealant therapy. Chlorhexidine may be used under fissure sealants for its antimicrobial effects without increasing microleakage.
Materials and Methods: In this in vitro study, 60 extracted molar teeth were randomly assigned to four groups (n=15): control group 1 (etch-and-rinse adhesive), control group 2 (self-etch adhesive), group 3 (etch-and-rinse adhesive and 0.2% chlorhexidine), and group 4 (self-etch adhesive and 0.2% chlorhexidine). After applying the sealant and subjecting the teeth to thermocycling, the teeth were immersed in 5% methylene blue solution. The teeth were sectioned into four parts and the dye penetration depth was measured under a stereomicroscope. Data were analyzed by the Mann–Whitney and Kruskal–Wallis tests using SPSS 24 (alpha=0.05).
Results: Microleakage was significantly lower in the self-etch adhesive system compared to the etch-and-rinse system in the control groups (P<0.05). For the etch-and-rinse adhesive, there was no significant difference in microleakage between the two groups with chlorhexidine (1.67±1.11) and without chlorhexidine (1.33±0.81) (P>0.05). Similarly, in the self-etch adhesive, microleakage did not significantly differ between groups with chlorhexidine (1.07±0.45) and without chlorhexidine (1.07±0.70) (P>0.05). The interaction effect of adhesive type and chlorhexidine on microleakage was not significant (P=0.428).
Conclusion: Application of chlorhexidine with both self-etch and etch-and-rinse adhesive systems did not significantly affect the microleakage of fissure sealant therapy. Chlorhexidine may be used under fissure sealants for its antimicrobial effects without increasing microleakage.
Type of Study: Original article |
Subject:
Restorative Dentistry
References
1. Liu W, Xiong L, Li J, Guo C, Fan W, Huang S. The anticaries effects of pit and fissure sealant in the first permanent molars of school-age children from Guangzhou: a population-based cohort study. BMC Oral Health. 2019 Jul 16;19(1):156. [DOI:10.1186/s12903-019-0846-x] [PMID] []
2. Papageorgiou SN, Dimitraki D, Kotsanos N, Bekes K, van Waes H. Performance of pit and fissure sealants according to tooth characteristics: A systematic review and meta-analysis. J Dent. 2017 Nov;66:8-17. [DOI:10.1016/j.jdent.2017.08.004] [PMID]
3. Wright JT, Crall JJ, Fontana M, Gillette EJ, Nový BB, Dhar V, et al. Evidence-based clinical practice guideline for the use of pit-and-fissure sealants: A report of the American Dental Association and the American Academy of Pediatric Dentistry. J Am Dent Assoc. 2016 Aug;147(8):672-682.e12. [DOI:10.1016/j.adaj.2016.06.001] [PMID]
4. Kühnisch J, Mansmann U, Heinrich-Weltzien R, Hickel R. Longevity of materials for pit and fissure sealing--results from a meta-analysis. Dent Mater. 2012 Mar;28(3):298-303. [DOI:10.1016/j.dental.2011.11.002] [PMID]
5. Paryab M. Effect of chlorhexidine solution on microleakage of pit and fissure sealants in permanent teeth with and without the use of fifth- and sixth-generation adhesive systems. Caspian J Dent Res 2023; 12 (2) :50-6.
6. Buyuk SK, Cantekin K, Demirbuga S, Ali Ozturk M. Are the low-shrinking composites suitable for orthodontic bracket bonding? Eur J Dent. 2013 Jul;7(3):284-8. [DOI:10.4103/1305-7456.115411] [PMID] []
7. Bao Z, Sun H, Fan D, Wang X, Wang Q. Shear bond strength and microleakage of pit and fissure sealants placed after saliva-contaminated etched enamel. Coatings. 2022;12(4):441. [DOI:10.3390/coatings12040441]
8. Esfahanizadeh G, Kooshki R, Karimipoor H, Rastin V. In Vitro Effects of Chlorhexidine and Listerine Mouthwashes on Color Stability of Glazed Bilayer Zirconia and IPS E.Max Ceramics. J Res Dent Maxillofac Sci. 2024;9(1):15-20 [DOI:10.61186/jrdms.9.1.15]
9. Rudolf JL, Moser C, Sculean A, Eick S. In-vitro antibiofilm activity of chlorhexidine digluconate on polylactide-based and collagen-based membranes. BMC Oral Health. 2019 Dec 26;19(1):291. [DOI:10.1186/s12903-019-0979-y] [PMID] []
10. Tavakolinejad Z, razeghi H, Sadeghi M. Effect of Pretreatment with Fluoride, Chlorhexidine, and Mixed Fluoride-Chlorhexidine Gels on Shear Bond Strength of Orthodontic Brackets: An Ex-Vivo Study. J Res Dent Maxillofac Sci 2021;6(1):24-9.
11. Bin-Shuwaish M, AlHussaini A, AlHudaithy L, AlDukhiel S, AlJamhan A, Alrahlah A. Effects of different antibacterial disinfectants on microleakage of bulk-fill composite bonded to different tooth structures. BMC Oral Health. 2021 Jul 16;21(1):348. [DOI:10.1186/s12903-021-01717-7] [PMID] []
12. Agrawal A, Shigli A. Comparison of six different methods of cleaning and preparing occlusal fissure surface before placement of pit and fissure sealant: an in vitro study. J Indian Soc Pedod Prev Dent. 2012 Jan-Mar;30(1):51-5. [DOI:10.4103/0970-4388.95582] [PMID]
13. Sobhi J, Hatam Zade E. Comparison of the Effect of Using Chlorhexidine Solution on Microleakage of Two Pit and Fissure Sealants. Jundishapur Sci Med J. 2015 May-June;14(2):189-98.
14. Shanmugaavel AK, Asokan S, John JB, Priya PG, Devi JG. Effect of One Percent Chlorhexidine Addition on the Antibacterial Activity and Mechanical Properties of Sealants: An in vitro Study. Int J Clin Pediatr Dent. 2015 Sep-Dec;8(3):196-201. [DOI:10.5005/jp-journals-10005-1312] [PMID] []
15. Memarpour M, Shafiei F. The effect of antibacterial agents on fissure sealant microleakage: a 6-month in vitro study. Oral Health Prev Dent. 2014;12(2):149-55.
16. Memarpour M, Abedinzade A, Rafiee A, Hashemian A. Penetration ability and microhardness of infiltrant resin and two pit and fissure sealants in primary teeth with early enamel lesions. Sci Rep. 2022 Mar 17;12(1):4652. [DOI:10.1038/s41598-022-08725-9] [PMID] []
17. Bayrak GD, Gurdogan-Guler EB, Yildirim Y, Ozturk D, Selvi-Kuvvetli S. Assessment of shear bond strength and microleakage of fissure sealant following enamel deproteinization: An in vitro study. J Clin Exp Dent. 2020 Mar 1;12(3):e220-6. [DOI:10.4317/jced.56281] [PMID] []
18. Hu YT, Yu F, Tang XY, Wu WZ, Zhang P, Hu ZH, et al. The antibacterial effect and physical performance of pit and fissure sealants based on an antibacterial core-shell nanocomposite. J Mech Behav Biomed Mater. 2021 May;117:104414. [DOI:10.1016/j.jmbbm.2021.104414] [PMID]
19. Klaisiri A, Vongsang J, Leelaudom T, Krajangta N. Methylene Blue Penetration of Resin Infiltration and Resin Sealant in Artificial White-Spot Lesions. Eur J Dent. 2023 Jul;17(3): 828-33. [DOI:10.1055/s-0042-1756689] [PMID] []
20. Al Khowaiter SS, Al-Bounni RS, Binalrimal S. Comparison of Dentinal Microleakage in Three Interim Dental Restorations: An In Vitro Study. J Int Soc Prev Community Dent. 2022 Dec 30;12(6):590-5. [DOI:10.4103/jispcd.JISPCD_183_21] [PMID] []
21. Shingare P, Chaugule V. An In Vitro Microleakage Study for Comparative Analysis of Two Types of Resin-based Sealants Placed by Using Three Different Types of Techniques of Enamel Preparation. Int J Clin Pediatr Dent. 2021 Jul-Aug;14(4):475-81. [DOI:10.5005/jp-journals-10005-1991] [PMID] []
22. Amend S, Frankenberger R, Boutsiouki C, Scharrelmann V, Winter J, Krämer N. Microleakage of pit and fissure sealings placed after enamel conditioning with phosphoric acid or with self-etching primers/adhesives. Clin Exp Dent Res. 2021 Oct;7(5):763-71. [DOI:10.1002/cre2.420] [PMID] []
23. Erfanparast L, Vatandoust M, Sohrabi A, Mahmoudi L. Clinical Comparison of Fissure Sealants Retention Following Preparation with Seventh Generation Bonding Agent with Prior Etching and Conventional Acid Etch and Bond System (fifth Generation). Dent Hypotheses. 2021 Jan- Mar;12(1):22-7. [DOI:10.4103/denthyp.denthyp_89_20]
24. Van Landuyt KL, Kanumilli P, De Munck J, Peumans M, Lambrechts P, Van Meerbeek B. Bond strength of a mild self-etch adhesive with and without prior acid-etching. J Dent. 2006 Jan;34(1):77-85. [DOI:10.1016/j.jdent.2005.04.001] [PMID]
25. Thangavelu A, Kaspar SS, Kathirvelu RP, Srinivasan B, Srinivasan S, Sundram R. Chlorhexidine: An Elixir for Periodontics. J Pharm Bioallied Sci. 2020 Aug;12(Suppl 1): S57-9. [DOI:10.4103/jpbs.JPBS_162_20] [PMID] []
26. Tavazo Zadeh E, Aref P, Askarizadeh N, Emadi F. In vitro Antimicrobial Effect of Punica granatum Extract versus Chlorhexidine on Streptococcus sobrinus, Streptococcus sanguinis, and Candida albicans. J Res Dent Maxillofac Sci 2023;8(1):18-27. [DOI:10.52547/jrdms.8.1.18]
27. Kapdan A, Öztaş N. Effects of chlorhexidine and gaseous ozone on microleakage and on the bond strength of dentin bonding agents with compomer restoration on primary teeth. J Dent Sci. 2015 March;10(1):46-54. [DOI:10.1016/j.jds.2013.02.026]
28. Haidari Gorji MA, Abolghasemzadeh F, Alaghemand H, Esmaeili B. Effect of 2% Chlorhexidine on the Enamel Microleakage of omposite Restorations Using 5th, 6th, 7th and Universal Generation of Dentine Bonding Agents (In Vitro). J Babol Univ Med Sci.2017;19(12):36-42.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
