Volume 9, Issue 1 (3-2024)                   J Res Dent Maxillofac Sci 2024, 9(1): 28-34 | Back to browse issues page


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Mokhtari H, Ghasemi N, Firouzi N, Soroush Barhaghi M H, Sokhandani D. Cleaning Efficacy of Mtwo and BioRaCe Rotary Systems: A Microbiological Evaluation. J Res Dent Maxillofac Sci 2024; 9 (1) :28-34
URL: http://jrdms.dentaliau.ac.ir/article-1-484-en.html
1- Department of Endodontics, Dental Faculty, Tabriz University of Medical Sciences, Tabriz, Iran. , mokhtari.z.h@gmail.com
2- Department of Endodontics, Dental Faculty, Tabriz University of Medical Sciences, Tabriz, Iran.
3- Department of Endodontics, Faculty of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
4- Department of Microbiology and Virology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract:   (670 Views)
Background and Aim: Effective cleaning and shaping of the root canal system is an essential part of a successful root canal treatment and is considered as one of the basic features of rotary instruments. The present study aimed to compare the efficacy of two types of nickel-titanium rotary files namely BioRaCe and Mtwo, in reduction of Enterococcus faecalis (E. faecalis) count in the root canal system.
Materials and Methods: In this in vitro study, 50 extracted human single-rooted teeth were assigned to two groups plus a positive and a negative control group (20 samples in each group). First, the root canals were sterilized and were then inoculated with E. faecalis (ATCC: 11700). Subsequently, all the root canals were instrumented with BioRaCe and Mtwo nickel-titanium rotary instruments. Then, samples were collected from inside the root canals and cultured in a special medium to evaluate the remaining colonies. The Chi-square goodness of fit test was used to assess the difference in bacterial colony count after root canal preparation with the two rotary systems (alpha=0.05). 
Results: Complete bacterial growth was observed in both the BioRaCe and Mtwo groups. There was no statistically significant difference between the two groups regarding bacterial elimination from the root canal system (P>0.05).
Conclusion: Under the limitations of the current study, the two rotary systems exhibited no superiority over each other in terms of their ability to eliminate microorganisms from the root canal system.
 
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Type of Study: Original article | Subject: Endodontics

References
1. Tabassum S, Khan FR. Failure of endodontic treatment: The usual suspects. Eur J Dent. 2016 Jan-Mar;10(1):144-7. [DOI:10.4103/1305-7456.175682] [PMID] []
2. Shadmehr E, Davoudi A, Damoon Sarmast N, Saatchi M. Evaluation of Antimicrobial Effcacy of Calcium Hypochlorite as an Endodontic Irrigant on a Mixed-Culture Biofilm: An Ex vivo Study. Iran Endod J. 2019 Summer;14(3):178-84.
3. Trairatvorakul C, Chunlasikaiwan S. Success of pulpectomy with zinc oxide-eugenol vs calcium hydroxide/iodoform paste in primary molars: a clinical study. Pediatr Dent. 2008 Jul-Aug;30(4):303-8.
4. Swapnil S, Sharma A, Shah N, Mandlik J, Ghogare A. Antimicrobial efficacy of triphala and curcumin extract in comparison with calcium hydroxide against E. faecalis as an intracanal medicament an study. Paripex Indian J Res. 2017 Jan;6(1):876-9.
5. Frough-Reyhani M, Ghasemi N, Soroush-Barhaghi M, Amini M, Gholizadeh Y. Antimicrobial efficacy of different concentration of sodium hypochlorite on the biofilm of Enterococcus faecalis at different stages of development. J Clin Exp Dent. 2016 Dec 1;8(5):e480-84. [DOI:10.4317/jced.53158] [PMID] []
6. Dutner J, Mines P, Anderson A. Irrigation trends among American Association of Endodontists members: a web-based survey. J Endod. 2012 Jan;38(1):37-40. [DOI:10.1016/j.joen.2011.08.013] [PMID]
7. Canga M, Malagnino VA, Rroco R, Xhemnica L, Budina R, Çelaj H, Kurti R. Maintenance Evaluation of the Original Anatomy of the Root Canal Diameters Instrumented with Mtwo and BioRace Systems. Maedica (Bucur). 2020 Dec;15(4):484-9.
8. Rodrigues RCV, Zandi H, Kristoffersen AK, Enersen M, Mdala I, Ørstavik D, Rôças IN, Siqueira JF Jr. Influence of the Apical Preparation Size and the Irrigant Type on Bacterial Reduction in Root Canal-treated Teeth with Apical Periodontitis. J Endod. 2017 Jul;43(7):1058-63. . [DOI:10.1016/j.joen.2017.02.004] [PMID]
9. Mokhtari H, Niknami M, Sohrabi A, Habibivand E, Mokhtari Zonouzi HR, Rahimi S, Zand V. Cone-Beam Computed Tomography Comparison of Canal Transportation after Preparation with BioRaCe and Mtwo Rotary Instruments and Hand K-Flexofiles. Iran Endod J. 2014 Summer;9(3):180-4.
10. Schäfer E, Erler M, Dammaschke T. Comparative study on the shaping ability and cleaning efficiency of rotary Mtwo instruments. Part 1. Shaping ability in simulated curved canals. Int Endod J. 2006 Mar;39(3):196-202. [DOI:10.1111/j.1365-2591.2006.01074.x] [PMID]
11. Zuolo ML, Zaia AA, Belladonna FG, Silva EJNL, Souza EM, Versiani MA, Lopes RT, De-Deus G. Micro-CT assessment of the shaping ability of four root canal instrumentation systems in oval-shaped canals. Int Endod J. 2018 May;51(5):564-71. [DOI:10.1111/iej.12810] [PMID]
12. Drukteinis S, Peciuliene V, Dummer PMH, Hupp J. Shaping ability of BioRace, ProTaper NEXT and Genius nickel-titanium instruments in curved canals of mandibular molars: a MicroCT study. Int Endod J. 2019 Jan;52(1):86-93. [DOI:10.1111/iej.12961] [PMID]
13. Cumbo E, Russo R, Gallina G. Assessment of root canal enlargement using Mtwo and BioRace rotary files. ScientificWorldJournal. 2015;2015:859693. [DOI:10.1155/2015/859693] [PMID] []
14. Coldero LG, McHugh S, MacKenzie D, Saunders WP. Reduction in intracanal bacteria during root canal preparation with and without apical enlargement. Int Endod J. 2002 May;35(5):437-46. [DOI:10.1046/j.1365-2591.2002.00496.x] [PMID]
15. Musani I, Goyal V, Singh A, Bhat C. Evaluation and comparison of biological cleaning efficacy of two endofiles and irrigants as judged by microbial quantification in primary teeth - an in vivo study. Int J Clin Pediatr Dent. 2009 Sep;2(3):15-22. [DOI:10.5005/jp-journals-10005-1013] [PMID] []
16. Siqueira JF Jr, Lima KC, Magalhães FA, Lopes HP, de Uzeda M. Mechanical reduction of the bacterial population in the root canal by three instrumentation techniques. J Endod. 1999 May;25(5):332-5. [DOI:10.1016/S0099-2399(06)81166-0] [PMID]
17. Zand V, Mokhtari H, Lotfi M, Rahimi S, Sohrabi A, Badamchi Zadeh S, Mojaver Kahnamooie H, Tehranchi P. A scanning electron microscope study on the effect of an experimental irrigation solution on smear layer removal. Iran Endod J. 2014 Spring;9(2):131-6.
18. Machado CAD, Souza ACA, Loureiro C, Martinho FC, Cintra LTÂ, Dezan Junior E, Jacinto RC. Comparison of two rotary systems in bacteria/lps removal from endodontic infections: randomized clinical trial. Braz Oral Res. 2019;33:e039. [DOI:10.1590/1807-3107bor-2019.vol33.0039] [PMID]
19. Yammine S, Jabbour E, Diemer F, Majzoub Z. Canal Straightening Following Overinstrumentation with Three Nickel-Titanium Rotary Instruments. J Int Soc Prev Community Dent. 2018 May-Jun;8(3):245-51. [DOI:10.4103/jispcd.JISPCD_18_18] [PMID] []
20. Nair PN. On the causes of persistent apical periodontitis: a review. Int Endod J. 2006 Apr;39(4):249-81. [DOI:10.1111/j.1365-2591.2006.01099.x] [PMID]
21. Siqueira JF Jr, Rôças IN, Favieri A, Lima KC. Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite. J Endod. 2000 Jun;26(6):331-4. [DOI:10.1097/00004770-200006000-00006] [PMID]
22. Nabavizadeh M, Abbaszadegan A, Khojastepour L, Amirhosseini M, Kiani E. A Comparison of Apical Transportation in Severely Curved Canals Induced by Reciproc and BioRaCe Systems. Iran Endod J. 2014 Spring;9(2):117-22.
23. Neves MA, Provenzano JC, Rôças IN, Siqueira JF Jr. Clinical Antibacterial Effectiveness of Root Canal Preparation with Reciprocating Single-instrument or Continuously Rotating Multi-instrument Systems. J Endod. 2016 Jan;42(1):25-9. [DOI:10.1016/j.joen.2015.09.019] [PMID]
24. Neves MAS, Provenzano JC, Fonseca SCL, Rodrigues RCV, Gonçalves LS, Siqueira JF Jr, Rôças IN. Disinfection and outcome of root canal treatment using single-file or multifile systems and Ca(OH)2 medication. Braz Dent J. 2020 Sep-Oct;31(5):493-8. [DOI:10.1590/0103-6440202003245] [PMID]
25. Saluja P, Mir S, Bavabeedu SS, Arora S, Abdulla AM, Baba SM. Relation between Apical Seal and Apical Preparation Diameter: An In Vitro Study. J Pharm Bioallied Sci. 2020 Aug;12(Suppl 1):S332-5. [DOI:10.4103/jpbs.JPBS_97_20] [PMID] []
26. Otero D, Selvaraju SB, Kathpalia A, Elmallah W, Elbatouty K, Nagy MM, et al. Evaluation of Bacterial Reduction after Root Canal Shaping Using ProTaper Gold and WaveOne Gold Rotary Systems. European Journal of General Dentistry. 2021 Aug;10(5):73-7. [DOI:10.1055/s-0041-1732953]
27. Krokidis A, Bonfanti C, Cerutti A, Barabanti N, Zinelis S, Panopoulos P. Comparative analysis of SAF, Protaper Next and BT-Race in eliminating Enterococcus faecalis from long oval canals: An ex vivo study. Aust Endod J. 2017 Dec;43(3):110-4. [DOI:10.1111/aej.12177] [PMID]
28. Matos Neto M, Santos SS, Leão MV, Habitante SM, Rodrigues JR, Jorge AO. Effectiveness of three instrumentation systems to remove Enterococcus faecalis from root canals. Int Endod J. 2012 May;45(5):435-8. [DOI:10.1111/j.1365-2591.2011.01994.x] [PMID]
29. Gorduysus M, Nagas E, Torun OY, Gorduysus O. A comparison of three rotary systems and hand instrumentation technique for the elimination of Enterococcus faecalis from the root canal. Aust Endod J. 2011 Dec;37(3):128-33. [DOI:10.1111/j.1747-4477.2010.00239.x] [PMID]
30. Canga M, Malagnino I, Malagnino G, Malagnino V. A Comparison of Mtwo and RaCe Rotary Instruments in the Preparation of Curved Canals. J Contemp Dent Pract. 2020 Feb 1;21(2):124-8. [DOI:10.5005/jp-journals-10024-2751] [PMID]
31. Regan JD, Gutmann JL, Witherspoon DE. Comparison of Diaket and MTA when used as root-end filling materials to support regeneration of the periradicular tissues. Int Endod J. 2002 Oct;35(10):840-7. [DOI:10.1046/j.1365-2591.2002.00582.x] [PMID]
32. Laslami K, Dhoum S, El Harchi A, Benkiran I. Relationship between the Apical Preparation Diameter and the Apical Seal: An In Vitro Study. Int J Dent. 2018 Jan 10;2018:2327854. [DOI:10.1155/2018/2327854] [PMID] []
33. Machado R, Nantes B, Guimarães DA. Influence of concentration, irrigation method, and root canal third on intratubular penetration of sodium hypochlorite - a broad statistical analysis. J Clin Exp Dent. 2023Nov 1;15(11):e882-e894. [DOI:10.4317/jced.60955] [PMID] []
34. Meyappan N, Mahadevan M, Manimaran ND. Scanning Electron Microscopy Analysis of Smear Layer Removal Ability of Conventional Endodontic Irrigation Regimen, MTAD, and QMix™ Versus a Mixture of Azadirachta indica and Citrus limon: An In Vitro Study. Cureus. 2023 Aug 2;15(8):e42877. [DOI:10.7759/cureus.42877] [PMID] []
35. Jurič IB, Anić I. The Use of Lasers in Disinfection and Cleanliness of Root Canals: a Review. Acta Stomatol Croat. 2014 Mar;48(1):6-15. [DOI:10.15644/asc48/1/1] [PMID] []
36. Azarpazhooh A, Limeback H. The application of ozone in dentistry: a systematic review of literature. J Dent. 2008 Feb;36(2):104-16. [DOI:10.1016/j.jdent.2007.11.008] [PMID]

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