Volume 8, Issue 3 (8-2023)                   J Res Dent Maxillofac Sci 2023, 8(3): 203-209 | Back to browse issues page

Ethics code: IR.MAZUMS.REC.1399.8114.

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hoshyari N, Mesgarani A, Shokrzadeh Lamuki M, Motafeghi F, Mousavi S J, Vaghar Mousavi M. Cytotoxicity of Two Calcium Silicate-Based Sealers and AH Plus Resin-Based Sealer for Human Gingival Fibroblasts. J Res Dent Maxillofac Sci 2023; 8 (3) :203-209
URL: http://jrdms.dentaliau.ac.ir/article-1-464-en.html
1- Department of Endodontics, School of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran , Narjeshoshyari@rocketmail.com
2- Department of Endodontics, School of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran
3- Department of Pharmacology and Toxicology, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
4- Department of Community Medicine, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
5- Private Dental Practice, Sari, Iran
Abstract:   (223 Views)
Background and Aim: The main goal of root canal treatment is to three-dimensionally seal the root canal system. Since sealer may contact the periapical tissue, it should be biocompatible and safe for the body. This study aimed to assess the cytotoxicity of MTA Fillapex and Endoseal MTA calcium silicate-based sealers and AH Plus resin-based sealer for human gingival fibroblasts (HGFs).   
Materials and Methods:  In this in vitro study, extracts of AH Plus, Endoseal MTA, and MTA Fillapex were obtained, serially diluted 1:2, 1:4, and 1:8, and were exposed to HGFs. Cytotoxicity was assessed by the methyl thiazolyl tetrazolium (MTT) assay. Data were analyzed by ANOVA and Tukey’s test (alpha=0.05).
Results: No significant difference existed between AH Plus 1:2 and MTA Fillapex 1:2 concentrations regarding the cell viability percentage (P>0.05). However, the difference between these two sealers in other concentrations was significant (P<0.05). No significant difference existed in AH Pus 1:4, Endoseal MTA 1:2, and MTA Fillapex 1:2 in cell viability (P>0.05); however, the difference among other concentrations of the three sealers was significant (P<0.05). The difference among AH Plus 1:8, Endoseal MTA 1:4, and MTA Fillapex 1:4 and 1:8 concentrations was not significant (P>0.05) but the difference among other concentrations was significant (P<0.05). Endoseal MTA 1:8 showed the highest and AH Plus 1:2 showed the lowest cell viability.
Conclusion: Endoseal MTA in all concentrations had lower cytotoxicity than MTA Fillapex and AH Plus and resulted in higher viability of HGFs.
Full-Text [PDF 687 kb]   (72 Downloads) |   |   Full-Text (HTML)  (61 Views)  
Type of Study: Original article | Subject: Endodontics

1. Tavares PB, Bonte E, Boukpessi T, Siqueira JF Jr, Lasfargues JJ. Prevalence of apical periodontitis in root canal-treated teeth from an urban French population: influence of the quality of root canal fillings and coronal restora-tions. J Endod. 2009 Jun;35(6):810-3.. [DOI:10.1016/j.joen.2009.03.048] [PMID]
2. Williamson AE, Marker KL, Drake DR, Dawson DV, Walton RE. Resin-based versus gutta-percha-based root canal obturation: influence on bacterial leakage in an in vitro model system. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Aug;108(2):292-6.. [DOI:10.1016/j.tripleo.2009.01.043] [PMID]
3. Lee JK, Kim S, Lee S, Kim HC, Kim E. In Vitro Comparison of Biocompatibility of Calcium Silicate-Based Root Canal Sealers. Materials (Basel). 2019 Jul 29;12(15):2411 [DOI:10.3390/ma12152411] [PMID] [PMCID]
4. Barbosa SV, Araki K, Spångberg LS. Cytotoxicity of some modi-fied root canal sealers and their leachable components. Oral Surg Oral Med Oral Pathol. 1993 Mar;75(3):357-61. [DOI:10.1016/0030-4220(93)90151-S] [PMID]
5. Sevimay S, Dalat D. Evaluation of penetration and adaptation of three different sealers: a SEM study. J Oral Rehabil. 2003 Sep;30(9):951-5. [DOI:10.1046/j.1365-2842.2003.01084.x] [PMID]
6. Kuga MC, Duarte MA, Sant'anna-Júnior A, Keine KC, Faria G, Dantas AA, Guiotti FA. Effects of calcium hydroxide addition on the physical and chemical properties of a calcium silicate-based sealer. J Appl Oral Sci. 2014 Jun; 22 (3):180-4. [DOI:10.1590/1678-775720130032] [PMID] [PMCID]
7. Bin CV, Valera MC, Camargo SE, Rabelo SB, Silva GO, Balducci I, Camargo CH. Cytotoxicity and genotoxicity of root canal sealers based on mineral trioxide aggregate. J Endod. 2012 Apr;38(4):495-500. [DOI:10.1016/j.joen.2011.11.003] [PMID]
8. Yoshino P, Nishiyama CK, Modena KC, Santos CF, Sipert CR. In vitro cytotoxicity of white MTA, MTA Fillapex® and Portland cement on human periodontal ligament fibroblasts. Braz Dent J. 2013;24(2):111-6. [DOI:10.1590/0103-6440201302115] [PMID]
9. Kim JA, Hwang YC, Rosa V, Yu MK, Lee KW, Min KS. Root Canal Filling Quality of a Premixed Calcium Silicate Endodontic Sealer Applied Using Gutta-percha Cone-mediated Ultrasonic Activation. J Endod. 2018 Jan;44 (1):133-8. [DOI:10.1016/j.joen.2017.07.023] [PMID]
10. Grela E, Kozłowska J, Grabowiecka A. Current methodology of MTT assay in bacteria - A review. Acta Histochem. 2018 May;120(4):303-11. [DOI:10.1016/j.acthis.2018.03.007] [PMID]
11. Yang R, Tian J, Huang X, Lei S, Cai Y, Xu Z, Wei X. A comparative study of dentinal tubule penetration and the re-treatability of EndoSequence BC Sealer HiFlow, iRoot SP, and AH Plus with different obturation techniques. Clin Oral Investig. 2021 Jun;25(6):4163-73. [DOI:10.1007/s00784-020-03747-x] [PMID] [PMCID]
12. Elsayed MA, Hassanien EE, Elgendy AAE. Ageing of TotalFill BC Sealer and MTA Fillapex in Simulated Body Fluid. Eur Endod J. 2021 Apr 14;6(2):183-8.
13. Dastorani M, Shourvarzi B, Nojoumi F, Ajami M. Comparison of Bacterial Microleakage of Endoseal MTA Sealer and Pro-Root MTA in Root Perforation. J Dent (Shiraz). 2021 Jun;22(2):96-101.
14. Yan P, Yuan Z, Jiang H, Peng B, Bian Z. Effect of bioaggregate on differentiation of human periodontal ligament fibroblasts. Int Endod J. 2010 Dec;43(12):1116-21. [DOI:10.1111/j.1365-2591.2010.01786.x] [PMID]
15. Shokrzadeh M, Mortazavi P, Moghadami A, Khayambashi B, Motafeghi F. Synergistic Antiproliferative and Anticancer Activi-ty of Carotenoid Lutein or Coenzyme Q10 in Combination with Doxorubicin on the MCF7 Cell Line. Applied In Vitro Toxicology. 2021;7(4):167-74. [DOI:10.1089/aivt.2021.0008]
16. Motafeghi F, Mortazavi P, Ghassemi-Barghi N, Zahedi M, Shokrzadeh M. Dexamethasone as an anti-cancer or hepatotoxic. Toxicol Mech Methods. 2022 Aug 8:1-11.
17. Ghorbani A, Sadeghnia HR, Asadpour E. Mechanism of pro-tective effect of lettuce against glucose/serum deprivation-induced neurotoxicity. Nutr Neurosci. 2015 Apr; 18(3):103-9. [DOI:10.1179/1476830513Y.0000000107] [PMID]
18. Tomson RM, Polycarpou N, Tomson PL. Contemporary obturation of the root canal system. Br Dent J. 2014 Mar; 216(6):315-22 [DOI:10.1038/sj.bdj.2014.205] [PMID]
19. GROSSMAN LI. An improved root canal cement. J Am Dent Assoc. 1958 Mar;56(3):381-5. [DOI:10.14219/jada.archive.1958.0055] [PMID]
20. Caviedes-Bucheli J, Castellanos F, Vasquez N, Ulate E, Munoz HR. The influence of two reciprocating single-file and two rota-ry-file systems on the apical extrusion of debris and its biologi-cal relationship with symptomatic apical periodontitis. A systematic review and meta-analysis. Int Endod J. 2016 Mar;49(3):255-70. [DOI:10.1111/iej.12452] [PMID]
21. Seo DG, Lee D, Kim YM, Song D, Kim SY. Biocompatibility and Mineralization Activity of Three Calcium Silicate-Based Root Canal Sealers Compared to Conventional Resin-Based Sealer in Human Dental Pulp Stem Cells. Materials (Basel). 2019 Aug 5;12(15):2482. [DOI:10.3390/ma12152482] [PMID] [PMCID]
22. Derakhshan S, Adl A, Parirokh M, Mashadiabbas F, Haghdoost AA. Comparing subcutaneous tissue responses to freshly mixed and set root canal sealers. Iran Endod J. 2009 Fall;4(4):152-7.
23. Rodríguez-Lozano FJ, García-Bernal D, Oñate-Sánchez RE, Ortolani-Seltenerich PS, Forner L, Moraleda JM. Evaluation of cytocompatibility of calcium silicate-based endo-dontic sealers and their effects on the biological responses of mesenchymal dental stem cells. Int Endod J. 2017 Jan;50(1):67-76. [DOI:10.1111/iej.12596] [PMID]
24. Adan A, Kiraz Y, Baran Y. Cell Proliferation and Cytotoxicity Assays. Curr Pharm Biotechnol. 2016;17(14): 1213-21. [DOI:10.2174/1389201017666160808160513] [PMID]
25. Jafari F, Jafari S. Composition and physicochemical properties of calcium silicate based sealers: A review article. J Clin Exp Dent. 2017 Oct 1;9(10):e1249-e55. [DOI:10.4317/jced.54103] [PMID] [PMCID]
26. Yoo YJ, Baek SH, Kum KY, Shon WJ, Woo KM, Lee W. Dynamic intratubular biomineralization following root canal obturation with pozzolan-based mineral trioxide aggregate sealer cement. Scanning. 2016 Jan-Feb;38(1):50-6. [DOI:10.1002/sca.21240] [PMID] [PMCID]
27. Kim MA, Rosa V, Neelakantan P, Hwang YC, Min KS. Characterization, Antimicrobial Effects, and Cytocompatibility of a Root Canal Sealer Produced by Pozzolan Reaction between Calcium Hydroxide and Silica. Materials (Basel). 2021 May 27;14(11):2863. [DOI:10.3390/ma14112863] [PMID] [PMCID]
28. Erdogan H, Yildirim S, Cobankara FK. Cytotoxicity and geno-toxicity of salicylate‐and calcium silicate‐based root canal seal-ers on primer human periodontal ligament fibroblasts. Aust Endod J. 2021 Dec;47(3):645-53. [DOI:10.1111/aej.12537] [PMID]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Journal of Research in Dental and Maxillofacial Sciences

Designed & Developed by: Yektaweb