Volume 8, Issue 1 (1-2023)
J Res Dent Maxillofac Sci 2023, 8(1): 18-27 |
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Ethics code: IR.IAU.DENTAL.REC.1399.126
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Tavazo Zadeh E, Aref P, Askarizadeh N, Emadi F. In vitro Antimicrobial Effect of Punica granatum Extract versus Chlorhexidine on Streptococcus sobrinus, Streptococcus sanguinis, and Candida albicans. J Res Dent Maxillofac Sci 2023; 8 (1) :18-27
URL: http://jrdms.dentaliau.ac.ir/article-1-395-en.html
URL: http://jrdms.dentaliau.ac.ir/article-1-395-en.html
1- Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2- Department of Pediatric Dentistry, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , parissa234@yahoo.com
3- Department of Pediatric Dentistry, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
4- Traditional Persian Medicine, Traditional Medicine Clinical Trial Research Center, Faculty of Medicine, Shahed University, Tehran, Iran
2- Department of Pediatric Dentistry, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , parissa234@yahoo.com
3- Department of Pediatric Dentistry, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
4- Traditional Persian Medicine, Traditional Medicine Clinical Trial Research Center, Faculty of Medicine, Shahed University, Tehran, Iran
Abstract: (830 Views)
Background and Aim: Considering the search for an effective antimicrobial agent comparable to chlorhexidine (CHX), this study aimed to assess the antimicrobial effect of Punica granatum (P. granatum) hydroalcoholic extract on Streptococcus sobrinus (S. sobrinus), Streptococcus sanguinis (S. sanguinis) and Candida albicans (C. albicans), in comparison with CHX.
Materials and Methods: In this in vitro study, the disc diffusion test was used to assess the antimicrobial activity of the extract by measuring the growth inhibition zones; while, the microdilution and macrodilution broth tests were applied to find the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract against the tested microorganisms. The MBC was measured using the blood agar or Mueller Hinton agar culture medium. The Sabouraud dextrose agar culture medium was used for C. albicans. Each test was repeated in triplicate, and data were analyzed by independent samples t-test and Mann-Whitney U test.
Results: None of the tested microorganisms showed any resistance to the extract. CHX had the highest antimicrobial effect against all tested microorganisms. The MIC of the hydroalcoholic extract of P. granatum was 2.5 mg/mL for S. sobrinus and S. sanguinis, and 5 mg/mL for C. albicans. Its MBC was 5 mg/mL for S. sobrinus and S. sanguinis, and 10 mg/mL for C. albicans. The mean diameter of the growth inhibition zone for S. sobrinus caused by CHX was significantly greater than that caused by P. granatum extract (Mann-Whitney U test, P=0.043). The same result was obtained for S. sanguinis (Student sample t-test, P=0.002), and C. albicans (Mann-Whitney U test, P=0.046).
Conclusion: The hydroalcoholic extract of P. granatum has bacteriostatic and bactericidal effects on S. sanguinis and S. sobrinus and antifungal effect on C. albicans comparable to CHX.
Materials and Methods: In this in vitro study, the disc diffusion test was used to assess the antimicrobial activity of the extract by measuring the growth inhibition zones; while, the microdilution and macrodilution broth tests were applied to find the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract against the tested microorganisms. The MBC was measured using the blood agar or Mueller Hinton agar culture medium. The Sabouraud dextrose agar culture medium was used for C. albicans. Each test was repeated in triplicate, and data were analyzed by independent samples t-test and Mann-Whitney U test.
Results: None of the tested microorganisms showed any resistance to the extract. CHX had the highest antimicrobial effect against all tested microorganisms. The MIC of the hydroalcoholic extract of P. granatum was 2.5 mg/mL for S. sobrinus and S. sanguinis, and 5 mg/mL for C. albicans. Its MBC was 5 mg/mL for S. sobrinus and S. sanguinis, and 10 mg/mL for C. albicans. The mean diameter of the growth inhibition zone for S. sobrinus caused by CHX was significantly greater than that caused by P. granatum extract (Mann-Whitney U test, P=0.043). The same result was obtained for S. sanguinis (Student sample t-test, P=0.002), and C. albicans (Mann-Whitney U test, P=0.046).
Conclusion: The hydroalcoholic extract of P. granatum has bacteriostatic and bactericidal effects on S. sanguinis and S. sobrinus and antifungal effect on C. albicans comparable to CHX.
Keywords: Pomegranate, Microbial Sensitivity Tests, Antibacterial Agents, Disk Diffusion Antimicrobial Tests, Biological Products
Type of Study: Original article |
Subject:
Restorative Dentistry
References
1. Löe H, Theilade E, Jensen SB, Schiott CR. Experimental gingivi-tis in man. J Periodontol (1930). 1965 May-Jun; 36: 177-87. [DOI:10.1902/jop.1965.36.3.177] [PMID]
2. Suhasini.SJ, Valiathan M. Brushing Techniques. Eur J Mol Clin Med. 2020;7(2):6601-11.
3. Waldron C, Nunn J, Mac Giolla Phadraig C, Comiskey C, Guerin S, van Harten MT, et al. Oral hygiene interventions for people with intellectual disabilities. Cochrane Database Syst Rev. 2019 May 31;5(5): CD012628. [DOI:10.1002/14651858.CD012628.pub2] [PMID] [PMCID]
4. Newman MG, Takei HH, Klokkevold PR, Carranza FA. New-man and Carranza's Clinical Periodontology. 13th ed. Philadelph-ia: Elsevier Saunders; 2019. 944 p.
5. Addy M. Chlorhexidine compared with other locally delivered antimicrobials. A short review. J Clin Periodontol. 1986 Nov;13(10):957-64. [DOI:10.1111/j.1600-051X.1986.tb01434.x] [PMID]
6. Akula S, Nagarathna J, Srinath K. Anti-Plaque and Anti-Gingivitis Efficacy of 0.25% Lemongrass Oil and 0.2% Chlorhexidine Mouthwash in Children. Front Dent. 2021 Sep 6;18:32.
7. Vyas T, Bhatt G, Gaur A, Sharma C, Sharma A, Nagi R. Chemical plaque control - A brief review. J Family Med Prim Care. 2021 Apr;10(4):1562-8. [DOI:10.4103/jfmpc.jfmpc_2216_20] [PMID] [PMCID]
8. Flötra L, Gjermo P, Rölla G, Waerhaug J. Side effects of chlor-hexidine mouth washes. Scand J Dent Res. 1971; 79(2): 119-25. [DOI:10.1111/j.1600-0722.1971.tb02001.x] [PMID]
9. Yarahmadi N, Hashemian F, Hosseini Doust R. Clinical Effects of Chlorhexidine 0.2% and Cetylpyridinium 0.05% Com-bination in Comparison with Chlorhexidine,Cetylpyridinium and Persica in Reducing Oral Bacteria in Healthy Individuals. J Pharm Care. 2020;8(3):116-22. [DOI:10.18502/jpc.v8i3.4545]
10. Pandiyan I, Rathinavelu PK, Arumugham MI, D S, Balasubramaniam A. Efficacy of Chitosan and Chlorhexidine Mouthwash on Dental Plaque and Gingival Inflammation: A Sys-tematic Review. Cureus. 2022 Mar 19;14(3):e23318. [DOI:10.7759/cureus.23318] [PMID] [PMCID]
11. Sreenivasan PK, Haraszthy VI. Chlorhexidine Improves Hy-giene Reducing Oral Polymorphonuclear Leukocytes with An-timicrobial Effects at Distinct Microenvironments amongst Subjects Stratified by Health Status. Antibiotics (Basel). 2022 Apr 29;11(5):603. [DOI:10.3390/antibiotics11050603] [PMID] [PMCID]
12. Gazi MI, Lambourne A, Chagla AH. The antiplaque effect of toothpaste containing salvadora persica compared with chlor-hexidine gluconate. Clin Prev Dent. 1987 Nov-Dec; 9 (6):3-8.
13. Zhao Y, Zhong WJ, Xun Z, Zhang Q, Song YQ, Liu YS, Chen F. Differences in carbon source usage by dental plaque in children with and without early childhood caries. Int J Oral Sci. 2017 Dec;9(12):e6. [DOI:10.1038/ijos.2017.47] [PMID] [PMCID]
14. Mungi S, Perona G. Genotypic concordance of streptocccus sobrinus ATCC® 33402TM in mother and child pairs detecting by molecular AP-PCR assays. J Dent Health Oral Disord Ther. 2019;10(6):310-5. [DOI:10.15406/jdhodt.2019.10.00504]
15. Rodriguez AM, Callahan JE, Fawcett P, Ge X, Xu P, Kitten T. Physiological and molecular characterization of genetic compe-tence in Streptococcus sanguinis. Mol Oral Microbiol. 2011 Apr;26(2):99-116. [DOI:10.1111/j.2041-1014.2011.00606.x] [PMID] [PMCID]
16. Blocker T. Analysis of the CYC1 Promoter in Candida Albi-cans [Thesis]. Langston: McCabe Thesis Collection; 2008. 35 p.
17. Anibal PC, Peixoto IT, Foglio MA, Höfling JF. Antifungal activi-ty of the ethanolic extracts of Punica granatum L. and evaluation of the morphological and structural modifications of its com-pounds upon the cells of Candida spp. Braz J Microbiol. 2013 Dec 17;44(3):839-48.
18. Vasconcelos LC, Sampaio FC, Sampaio MC, Pereira Mdo S, Higino JS, Peixoto MH. Minimum inhibitory concentration of adherence of Punica granatum Linn (pomegranate) gel against S. mutans, S. mitis and C. albicans. Braz Dent J. 2006;17(3):223-7. [DOI:10.1590/S0103-64402006000300009] [PMID]
19. Lavaee F, Motaghi D, Jassbi AR, Jafarian H, Ghasemi F, Badiee P. Antifungal effect of the bark and root extracts of Punica gran-atum on oral Candida isolates. Curr Med Mycol. 2018 Dec;4(4):20-4. [DOI:10.18502/cmm.4.4.382] [PMID] [PMCID]
20. Jain E, Pandey RK, Khanna R. Liquorice root extracts as po-tent cariostatic agents in pediatric practice. J Indian Soc Pedod Prev Dent. 2013 Jul-Sep;31(3):146-52. [DOI:10.4103/0970-4388.117964] [PMID]
21. Sedighinia F, Safipour Afshar A, Soleimanpour S, Zarif R, Asili J, Ghazvini K. Antibacterial activity of Glycyrrhiza glabra against oral pathogens: an in vitro study. Avicenna J Phytomed. 2012 Summer;2(3):118-24.
22. Ajagannanavar SL, Battur H, Shamarao S, Sivakumar V, Patil PU, Shanavas P. Effect of aqueous and alcoholic licorice (glycyr-rhiza glabra) root extract against streptococcus mutans and lactobacillus acidophilus in comparison to chlor-hexidine: an in vitro study. J Int Oral Health. 2014 Jul;6(4):29-34. [DOI:10.4103/2231-0762.146215] [PMID] [PMCID]
23. Caiyun W, Ling-ling SH, Litong F, Youfang D, Shan Z, Yujun L, et al. Optimization of extraction and enrichment of phenolics from pomegranate (Punica granatum L.) leaves. Ind Crops Prod. 2013; 42:587-94. [DOI:10.1016/j.indcrop.2012.06.031]
24. Humphries RM, Ambler J, Mitchell SL, Castanheira M, Dingle T, Hindler JA, et al. CLSI Methods Development and Standardiza-tion Working Group of the Subcommittee on Antimicrobial Susceptibility Testing. CLSI Methods Development and Standardization Working Group Best Practices for Evaluation of Antimicrobial Susceptibility Tests. J Clin Microbiol. 2018 Mar 26;56(4):e01934-17.
25. Rahimi VB, Askari VR, Shirazinia R, Soheili-Far S, Askari N, Rahmanian-Devin P, Sanei-Far Z, Mousavi SH, Ghodsi R. Protec-tive effects of hydro-ethanolic extract of Terminalia chebula on primary microglia cells and their polarization (M1/M2 balance). Mult Scler Relat Disord. 2018 Oct;25:5-13. [DOI:10.1016/j.msard.2018.07.015] [PMID]
26. Wikler MA, Cockerill FR, Bush K, Dudley MN, Eliopoulos GM, Hardy DJ, et al. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. Approved Standard M7-A8. 2009.
27. Alexander BD, Procop GW, Dufresne P, Fuller J, Ghannoum MA, Hanson KE, et al. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts-4th ed. Clin Lab Stand Inst. 2017;37(13):M27-A3.
28. Miller RA, Gaunt PS, Burbick CR, Avendaño-Herrera R, Buller N, Chuanchuen R, et al. Methods for Antimicrobial Broth Dilu-tion and Disk Diffusion Susceptibility Testing of Bacteria Isolat-ed From Aquatic Animals. 2nd ed. Clin Lab Stand Inst. 2020;40(3):VET03-A.
29. Petrus EM, Tinakumari S, Chai LC, Ubong A, Tunung R, Elexson N, et al. A study on the minimum inhibitory concentration and minimum bactericidal concentration of Nano Colloidal Silver on food-borne pathogens. Int Food Res J. 2011;18(1):55-66.
30. Shaygannia E, Bahmani M, Zamanzad B, Rafieian-Kopaei M. A Review Study on Punica granatum L. J Evid Based Complementary Altern Med. 2016 Jul;21(3):221-7. [DOI:10.1177/2156587215598039] [PMID]
31. Benslimane S, Rebai O, Djibaoui R, Arabi A. Pomegranate Peel Extract Activities as Antioxidant and Antibiofilm against Bacte-ria Isolated from Caries and Supragingival Plaque. Jordan J Biol Sci. 2020;13(3):403-12.
32. Duman AD, Ozgen M, Dayisoylu KS, Erbil N, Durgac C. Antimi-crobial activity of six pomegranate (Punica granatum L.) varie-ties and their relation to some of their pomological and phyto-nutrient characteristics. Molecules. 2009 May 13; 14(5):1808-17. [DOI:10.3390/molecules14051808] [PMID] [PMCID]
33. Yu M, Gouvinhas I, Barros A. Variation of the Polyphenolic Composition and Antioxidant Capacity of Freshly Prepared Pomegranate Leaf Infusions over One-Day Storage. Antioxidants (Basel). 2021 Jul 26;10(8):1187. [DOI:10.3390/antiox10081187] [PMID] [PMCID]
34. Dahham SS, Ali MN, Tabassum H, Khan M. Studies on antibac-terial and antifungal activity of pomegranate (Punica granatum L.). Am-Euras. J. Agric. & Environ. Sci. 2010;9(3): 273-81.
35. Endo EH, Cortez DA, Ueda-Nakamura T, Nakamura CV, Dias Filho BP. Potent antifungal activity of extracts and pure com-pound isolated from pomegranate peels and synergism with fluconazole against Candida albicans. Res Microbiol. 2010 Sep;161(7):534-40. [DOI:10.1016/j.resmic.2010.05.002] [PMID]
36. Braga LC, Shupp JW, Cummings C, Jett M, Takahashi JA, Carmo LS, Chartone-Souza E, Nascimento AM. Pomegranate extract inhibits Staphylococcus aureus growth and subsequent enterotoxin production. J Ethnopharmacol. 2005 Jan 4;96(1-2):335-9. [DOI:10.1016/j.jep.2004.08.034] [PMID]
37. Peršurić Ž, Saftić Martinović L, Malenica M, Gobin I, Pedisić S, Dragović-Uzelac V, Kraljević Pavelić S. Assessment of the Biological Activity and Phenolic Composition of Ethanol Extracts of Pomegranate (Punica granatum L.) Peels. Molecules. 2020 Dec 14;25(24):5916. [DOI:10.3390/molecules25245916] [PMID] [PMCID]
38. Rosas-Burgos EC, Burgos-Hernández A, Noguera-Artiaga L, Kačániová M, Hernández-García F, Cárdenas-López JL, Car-bonell-Barrachina ÁA. Antimicrobial activity of pomegranate peel extracts as affected by cultivar. J Sci Food Agric. 2017 Feb;97(3):802-10. [DOI:10.1002/jsfa.7799] [PMID]
39. Gosset-Erard C, Zhao M, Lordel-Madeleine S, Ennahar S. Iden-tification of punicalagin as the bioactive compound behind the antimicrobial activity of pomegranate (Punica gran-atum L.) peels. Food Chem. 2021 Aug 1;352:129396. [DOI:10.1016/j.foodchem.2021.129396] [PMID]
40. Estabraghi E, Sadeghpour M, Mehrabani A. Study of pomegranate hydromethanol extract on Staphylococcus aureus and Escherichia coli by microplate in laboratory conditions. J Payavard Salamat. 2018;12(3):183-92.
41. Ismail T, Sestili P, Akhtar S. Pomegranate peel and fruit ex-tracts: a review of potential anti-inflammatory and anti-infective effects. J Ethnopharmacol. 2012 Sep 28;143 (2):397-405. [DOI:10.1016/j.jep.2012.07.004] [PMID]
42. Lalwani V, Koneru A, Vanishree M, Vardendra M, Hunasgi S, Surekha R. Anti-microbial activity of Punica granatum on Strep-tococcus in dental caries patients and healthy individuals: A comparative study. J Adv Clin Res Insights. 2014;1(3):94-8. [DOI:10.15713/ins.jcri.24]
43. Mehdipour Biregani Z, Sharifan A. Extraction of bioactive compounds from pomegranate: A review. J Food Bioprocess Eng. 2021;4(1):37-42.
44. Mirjalili A. A review on biochemical constituents and medicinal properties of pomegranate (Punica granatum L.). J. Med. Plants 2015;14(56):1-22.
45. Veloso DJ, Abrão F, Martins CHG, Bronzato JD, Gomes BPFA, Higino JS, Sampaio FC. Potential antibacterial and anti-halitosis activity of medicinal plants against oral bacteria. Arch Oral Biol. 2020 Feb;110:104585. [DOI:10.1016/j.archoralbio.2019.104585] [PMID]
46. Ferrazzano GF, Scioscia E, Sateriale D, Pastore G, Colicchio R, Pagliuca C, Cantile T, Alcidi B, Coda M, Ingenito A, Scaglione E, Cicatiello AG, Volpe MG, Di Stasio M, Salvatore P, Pagliarulo C. In Vitro Antibacterial Activity of Pomegranate Juice and Peel Extracts on Cariogenic Bacteria. Biomed Res Int. 2017;2017:2152749. [DOI:10.1155/2017/2152749] [PMID] [PMCID]
47. Nozohour Y, Golmohammadi R, Mirnejad R, Fartashvand M. Antibacterial activity of pomegranate (Punica granatum L.) seed and peel alcoholic extracts on Staphylococcus aureus and Pseudomonas aeruginosa isolated from health cen-ters. J Appl Biotechnol Rep. 2018;5(1):32-6. [DOI:10.29252/JABR.01.01.06]
48. Millo G, Juntavee A, Ratanathongkam A, Nualkaew N, Peerapattana J, Chatchiwiwattana S. Antibacterial Inhibitory Effects of Punica Granatum Gel on Cariogenic Bacteria: An in vitro Study. Int J Clin Pediatr Dent. 2017 Apr-Jun;10(2):152-7. [DOI:10.5005/jp-journals-10005-1426] [PMID] [PMCID]
49. Aravindraj S, Preethi M, Sivapathasundharam B. Antimicrobial Effects of Punica granatum Extracts on Staphylo-coccus aureus, Streptococcus mutans, Lactobacillus acidophi-lus, Enterococcus faecalis and Candida albicans. Int J Curr Mi-crobiol Appl Sci. 2017;6(9):2762-74. [DOI:10.20546/ijcmas.2017.609.341]
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