Volume 4, Issue 1 (Journal of Research in Dental & Maxillofacial Sciences Winter 2019)                   J Res Dent Maxillofac Sci 2019, 4(1): 1-8 | Back to browse issues page

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Alaee A, Azizi A, valaei N, Taheri S, Ravanshadi F, Hajipour M et al . In-Vitro Effect of Natural Honey on the Growth of Streptococcus sanguinis. J Res Dent Maxillofac Sci. 2019; 4 (1) :1-8
URL: http://jrdms.dentaliau.ac.ir/article-1-222-en.html
1- Assistant Professor, Oral medicine Dept, Member of Dental Material Research Center,Dental Faculty
2- Professor,Oral Medicine Dept,Dental Faculty
3- BSC, Faculty member of Thalasemia Research center
4- Associate Professor Dept microbiology
5- Dentist
6- Post Grduated Students, Oral Medicine Dept, Faculty of Dentistry , Maryam.fattahi2010@gmail.com
Abstract:   (2768 Views)
Background and Aim:  Streptococcus sanguinis (S. sanguinis) is one of the effective bacteria in the formation of caries and periodontal diseases which are caused by dental plaque. Currently, the use of natural honey (NH) is considered as a method of reducing S. sanguinis and S. mutans in saliva, which results in reducing the dental plaque. The purpose of this study was to evaluate the effects of NH on the growth of S. sanguinis and to compare its effects with that of Ciprofloxacin and Penicillin antibiotics in vitro.  
Materials and Methods: In this experimental study, the effect of NH against S. sanguinis was investigated according to the growth inhibition zone in the disk diffusion method by measuring the inhibition zone around the disks which were dipped in the case and control groups. The agar well diffusion method was used as well. The results were analyzed using analysis of variance (ANOVA). In case of the presence of a significant difference, post-hoc Scheffe test was used as a supplementary test.
Results: In the disk diffusion method, the average inhibition zone was 21 mm for Ciprofloxacin, 10.35 mm for Penicillin, and 17.77 mm for NH (P<0.001). In the agar well diffusion method, the average inhibition zone was 17.68 mm for Ciprofloxacin, 15.83 mm for NH, and 8.06 mm for Penicillin (P<0.01).  
Conclusion: It seems that NH is effective against S. sanguinis in vitro. Nevertheless, its inhibition zone in the agar well diffusion and the disk diffusion methods is smaller than that formed by Ciprofloxacin and larger than that formed by Penicillin.
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Type of Study: Original article | Subject: Oral medicine

1. He XS, Shi WY. Oral microbiology: past, present and future. Int J Oral Sci. 2009 Jun;1(2):47-58. [DOI:10.4248/ijos.09029] [PMID] [PMCID]
2. Marsh PD. Microbiology of dental plaque biofilms and their role in oral health and caries. Dent Clin North Am. 2010 Jul;54(3):441-54. [DOI:10.1016/j.cden.2010.03.002] [PMID]
3. Aas JA, Griffen AL, Dardis SR, Lee AM, Olsen I, Dewhirst FE, et al. Bacteria of dental caries in primary and permanent teeth in children and young adults. J Clin Microbiol. 2008 Apr;46(4):1407-17. [DOI:10.1128/JCM.01410-07] [PMID] [PMCID]
4. Yim N, Ha do T, Trung TN, Kim JP, Lee S, Na M, et al. The antimicrobial activity of compounds from the leaf and stem of Vitis amurensis against two oral pathogens. Bioorg Med Chem Lett. 2010 Feb 1;20(3):1165-8. [DOI:10.1016/j.bmcl.2009.12.020] [PMID]
5. Doyle RJ, Nesbitt WE, Grant Taylor K. On the mechanism of adherence of Streptococcus sanguis to hydroxylapatite. FEMS Microbiol Lett. 1982 Sep;15(1):1-5. [DOI:10.1111/j.1574-6968.1982.tb00027.x]
6. Giacaman RA, Torres S, Gomez Y, Munoz-Sandoval C, Kreth J. Correlation of Streptococcus mutans and Streptococcus sanguinis colonization and ex vivo hydrogen peroxide production in carious lesion-free and high caries adults. Arch Oral Biol. 2015 Jan;60(1):154-9. [DOI:10.1016/j.archoralbio.2014.09.007] [PMID]
7. Rotilie CA, Fass RJ, Prior RB, Perkins RL. Microdilution technique for antimicrobial susceptibility testing of anaerobic bacteria. Antimicrob Agents Chemother. 1975 Mar;7(3):311-15. [DOI:10.1128/AAC.7.3.311] [PMID] [PMCID]
8. Featherstone JD. Caries prevention and reversal based on the caries balance. Pediatr Dent. 2006 Mar 1;28(2):128-32.
9. Abd El-Malek FF, Yousef AS, El-Assar SA. Hydrogel film loaded with new formula from manuka honey for treatment of chronic wound infections. J Glob Antimicrob Resist. 2017 Dec;11:171-176. [DOI:10.1016/j.jgar.2017.08.007] [PMID]
10. Singhal R, Siddibhavi M, Sankeshwari R, Patil P, Jalihal S, Ankola A. Effectiveness of three mouthwashes - Manuka honey, Raw honey, and Chlorhexidine on plaque and gingival scores of 12-15-year-old school children: A randomized controlled field trial. J Indian Soc Periodontol. 2018 Jan-Feb;22(1):34-39.
11. Mathai K, Anand S, Aravind A, Dinatius P, Krishnan AV, Mathai M. Antimicrobial Effect of Ginger, Garlic, Honey, and Lemon Extracts on Streptococcus mutans. J Contemp Dent Pract. 2017 Nov 1;18(11):1004-1008. [DOI:10.5005/jp-journals-10024-2165] [PMID]
12. Molan PC. The role of honey in the management of wounds. J Wound Care. 1999 Sep;8(8):415-8. [DOI:10.12968/jowc.1999.8.8.25904] [PMID]
13. Nejabat M, Astaneh A, Eghtedari M, Mosallaei M, Ashraf MJ, Mehrabani D. Effect of honey in Pseudomonas aeruginosa induced stromal keratitis in rabbits. J Appl Animal Res. 2009 Jun 1;35(2):101-4. [DOI:10.1080/09712119.2009.9706996]
14. Schmidlin PR, English H, Duncan W, Belibasakis GN, Thurnheer T. Antibacterial potential of Manuka honey against three oral bacteria in vitro. Swiss Dent J. 2014;124(9):922-4.
15. Leandro LF, Mendes CA, Casemiro LA, Vinholis AH, Cunha WR, de Almeida R, et al. Antimicrobial activity of apitoxin, melittin and phospholipase A2 of honey bee (Apis mellifera) venom against oral pathogens. An Acad Bras Cienc. 2015 Mar;87(1):147-55. [DOI:10.1590/0001-3765201520130511] [PMID]
16. Andualem B. Combined antibacterial activity of stingless bee (Apis mellipodae) honey and garlic (Allium sativum) extracts against standard and clinical pathogenic bacteria. Asian Pac J Trop Biomed. 2013 Sep;3(9):725-31. [DOI:10.1016/S2221-1691(13)60146-X]
17. Molan PC. The antibacterial activity of honey: 1. The nature of the antibacterial activity. Bee World. 1992 Jan 1;73(1):5-28. [DOI:10.1080/0005772X.1992.11099109]
18. Bauer L, Kohlich A, Hirschwehr R, Siemanna U, Ebner H, Scheiner O, et al. Food allergy to honey: Pollen or bee products? Characterization of allergenic proteins in honey by means of immunoblotting. J Allergy Clin Immunol. 1996 Jan 1;97(1):65-73. [DOI:10.1016/S0091-6749(96)70284-1]
19. Duailibe SA, Gonçalves AG, Ahid FJ. Effect of a propolis extract on Streptococcus mutans counts in vivo. J Appl Oral Sci. 2007 Oct;15(5):420-3. [DOI:10.1590/S1678-77572007000500009] [PMID] [PMCID]
20. Sela MO, Maroz D, Gedalia I. Streptococcus Mutans in saliva of normal subjects and neck and head irradiated cancer subject after consumption of honey. J Oral Rehabil. 2000;27:269-270. [DOI:10.1046/j.1365-2842.2000.00504.x] [PMID]
21. Performance Standards for Antimicrobial Susceptibility Testing. 28th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute; 2018.
22. Vahabi S, Najafi E, Alizadeh S. In vitro antimicrobial effects of some herbal essences against oral pathogens. J Med Plants Res. 2011;5(19):4870-8.
23. Khozeymeh F, Golestannejad Z, Aayan A. Antibacterial Effect of Astrangalus Honey on Cariogenic Lactobacillus Strains. J Isfahan Dent Sch. 2016;11(6):45-62.
24. Allen KL, Molan PC, Reid GM. A survey of the antibacterial activity of some New Zealand honeys. J Pharm Pharmacol. 1991 Dec;43(12):817-822. [DOI:10.1111/j.2042-7158.1991.tb03186.x] [PMID]
25. Ghotaslou R, Saghati H, Dehnad A, Salahi Eshlaghi B. Antibacterial Effect of Azerbaijan Honey on Pseudomonas aeroginosa Biofilm. Iran J Med Microbiol. 2016;9(4):40-6.
26. Atwa AD, AbuShahba RY, Mostafa M, Hashem MI. Effect of honey in preventing gingivitis and dental caries in patients undergoing orthodontic treatment. Saudi Dent J. 2014 Jul;26(3):108-14. [DOI:10.1016/j.sdentj.2014.03.001] [PMID] [PMCID]
27. Khosravi-Darani K, Khaksar R, Esmaeili S, Seyed-Reihani F, Zoghi A, Shahbazizadeh S. Antifungal and Anti-bacterial Synergistic Effects of Mixture of Honey and Herbal Extracts. ZJRMS. 2013;15(8):30-33.
28. Kwakman PH, Te Velde AA, de Boer L, Vandenbroucke-Grauls CM, Zaat SA. Two major medicinal honeys have different mechanisms of bactericidal activity. PloS One. 2011 Mar 4;6(3):e17709. [DOI:10.1371/journal.pone.0017709] [PMID] [PMCID]
29. Nassar HM, Li M, Gregory RL. Effect of honey on Streptococcus mutans growth and biofilm formation. Appl Environ Microbiol. 2012 Jan;78(2):536-40. [DOI:10.1128/AEM.05538-11] [PMID] [PMCID]
30. Moezzi ghadim N, Taghibakhsh M, Godarzi H, Liravinezhad hoseini N, Alirezaei S. Evaluation of the Effect of Four Herbal Extracts on Growth of Streptococcus mutans and Lactobacillus. J Res Dentomaxillofac Sci. 2018;3(2):7-13. [DOI:10.29252/jrdms.3.2.7]
31. Shoae Hassani AR, Hamdi K, Ghaemi A. In vitro reduction in colonization of Streptococcus mutans by honey beeswax ethyl acetate extract. AMUJ. 2009 Winter;11(4):87-95.
32. Jain A, Bhaskar DJ, Gupta D, Agali C, Gupta V, Gupta RK, et al. Comparative evaluation of honey, chlorhexidine gluconate (0.2%) and combination of xylitol and chlorhexidine mouthwash (0.2%) on the clinical level of dental plaque: A 30 days randomized control trial. Perspect Clin Res. 2015 Jan-Mar;6(1):53-7. [DOI:10.4103/2229-3485.148819] [PMID] [PMCID]

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