Volume 3, Issue 2 (4-2018)
J Res Dent Maxillofac Sci 2018, 3(2): 37-45 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Farhadi S, Shahsavari F, Alf K. Comparison of Expression of p53 and bcl-2 Markers in Oral Lichen Planus and Oral Squamous Cell Carcinoma. J Res Dent Maxillofac Sci 2018; 3 (2) :37-45
URL: http://jrdms.dentaliau.ac.ir/article-1-206-en.html
URL: http://jrdms.dentaliau.ac.ir/article-1-206-en.html
1- Assistant professor, Oral & Maxillofacial Pathology Dept, Dental Branch of Tehran,
2- Assistant professor, Oral & Maxillofacial pathology dept, Dental branch of Tehran, ,dr.f.shahsavari@gmail.com
3- Dentist
2- Assistant professor, Oral & Maxillofacial pathology dept, Dental branch of Tehran, ,
3- Dentist
Abstract: (3330 Views)
Background and Aim: It has been suggested that oral lichen planus (OLP) can be a potential premalignant lesion. Reports are also available on the role of bcl-2 and p53 proteins in malignant transformation of OLP into oral squamous cell carcinoma (OSCC). Due to controversies in these reports, the present study aimed to compare the expression of p53 and bcl-2 markers in OLP and OSCC.
Materials and Methods: In this experimental study, immunohistochemical (IHC) staining was performed on erosive OLP and well-differentiated OSCC samples using bcl-2 and p53 antibodies. One-thousand cells per slide were counted, and the results were reported as percentages. The results were scored, and Mann-Whitney U test was used to compare staining grades in the two lesions.
Results: Twenty-two OLP and 25 OSCC samples with a mean age of 49.3±15.8 years were evaluated. Bcl-2 expression was reported to be 16.27±8.95% in OLP and 16.4±22.9% in OSCC. Expression of p53 was 30.86±28.26% in OLP and 49.6±29.6% in OSCC. The difference in bcl-2 expression between the two lesions was not significant (P=0.266), whereas this difference was significant for p53 expression (P=0.02). The correlation coefficient between these two markers was reported to be 0.45 in OSCC and 0.1 in OLP.
Conclusion: According to the results, there was a significant difference in p53 expression between OLP and OSCC samples, whereas this difference was not significant for bcl-2. There was no significant association between the expressions of these two markers in the samples. Therefore, there does not seem to be a high malignancy potential for the studied OLP samples.
Materials and Methods: In this experimental study, immunohistochemical (IHC) staining was performed on erosive OLP and well-differentiated OSCC samples using bcl-2 and p53 antibodies. One-thousand cells per slide were counted, and the results were reported as percentages. The results were scored, and Mann-Whitney U test was used to compare staining grades in the two lesions.
Results: Twenty-two OLP and 25 OSCC samples with a mean age of 49.3±15.8 years were evaluated. Bcl-2 expression was reported to be 16.27±8.95% in OLP and 16.4±22.9% in OSCC. Expression of p53 was 30.86±28.26% in OLP and 49.6±29.6% in OSCC. The difference in bcl-2 expression between the two lesions was not significant (P=0.266), whereas this difference was significant for p53 expression (P=0.02). The correlation coefficient between these two markers was reported to be 0.45 in OSCC and 0.1 in OLP.
Conclusion: According to the results, there was a significant difference in p53 expression between OLP and OSCC samples, whereas this difference was not significant for bcl-2. There was no significant association between the expressions of these two markers in the samples. Therefore, there does not seem to be a high malignancy potential for the studied OLP samples.
Type of Study: Original article |
References
1. Leyva-Huerta ER, Ledesma-Montes C, RojoBotello RE, Vega-Memije E. P53 and bcl-2 immunoexpression in patients with oral lichen planus and oral squamous cell carcinoma. Med Oral Patol Oral Cir Bucal. 2012 Sep 1;17(5):e745-50. [DOI:10.4317/medoral.18013] [PMID] [PMCID]
2. Sadri D, Azizi A, Farhadi S, Shokrgozar H, Entezari N. Head and neck metastatic tumors: a retrospective survey of Iranian patients. J Dent (Shiraz). 2015 Mar;16(1):17-21.
3. de Sousa FA, Paradella TC, Carvalho YR, Rosa LE. Immunohistochemical expression of PCNA, p53, bax and bcl-2 in oral lichen planus and epithelial dysplasia. J Oral Sci. 2009 Mar;51(1):117-21. [DOI:10.2334/josnusd.51.117] [PMID]
4. de Sousa FA, Paradella TC, Carvalho YR, Rosa LE. Comparative analysis of the expression of proliferating cell nuclear antigen, p53, bax, and bcl-2 in oral lichen planus and oral squamous cell carcinoma. Ann Diagn Pathol. 2009 Oct;13(5):308-12. [DOI:10.1016/j.anndiagpath.2009.06.001] [PMID]
5. Hussein MR, Al-Badaiwy ZH, Guirguis MN. Analysis of p53 and bcl-2 protein expression in the non-tumorigenic, pretumorigenic and tumorigenic keratinocytic hyperproliferative lesions. J Cutan Pathol. 2004 Nov;31(10):643-51. [DOI:10.1111/j.0303-6987.2004.00244.x] [PMID]
6. Shirasuna K. Oral lichen planus: Malignant potential and diagnosis. Oral Sci Int. 2014 Jan 1;11(1):1-7. [DOI:10.1016/S1348-8643(13)00030-X]
7. Irani S, Esfahani AM, Ghorbani A. Dysplastic change rate in cases of oral lichen planus: A retrospective study of 112 cases in an Iranian population. J Oral Maxillofac Pathol. 2016 SepDec;20(3):395-399. [DOI:10.4103/0973-029X.190911] [PMID] [PMCID]
8. Gonzalez-Moles MA, Galindo P, GutierrezFernandez J, Sanchez-Fernandez E, RodriguezArchilla A, Ruiz-Avila I, et al. P53 protein expression in oral squamous cell carcinoma. Survival analysis. Anticancer Res. 2001 JulAug;21(4B):2889-94.
9. van der Meij EH, Mast H, van der Waal I. The possible premalignant character of oral lichen planus and oral lichenoid lesions: a prospective five-year follow-up study of 192 patients. Oral Oncol. 2007 Sep;43(8):742-8. [DOI:10.1016/j.oraloncology.2006.09.006] [PMID]
10. Hsue SS, Wang WC, Chen CH, Lin CC, Chen YK, Lin LM. Malignant transformation in 1458 patients with potentially malignant oral mucosal disorders: a follow-up study based in a Taiwanese hospital. J Oral Pathol Med. 2007 Jan;36(1):25-9. [DOI:10.1111/j.1600-0714.2006.00491.x] [PMID]
11. Shahsavari F, Sadri D, Tabari SS. Expression of P27 Protein in Oral and Esophageal Squamous Cell Carcinoma. J Mash Dent Sch. 2012;36(3):203-10.
12. Neppelberg E, Johannessen AC, Jonsson R. Apoptosis in oral lichen planus. Eur J Oral Sci. 2001 Oct;109(5):361-4. [DOI:10.1034/j.1600-0722.2001.00081.x] [PMID]
13. Jordan RC, Catzavelos GC, Barrett AW, Speight PM. Differential expression of bcl2 and bax in squamous cell carcinomas of the oral cavity. Eur J Cancer B Oral Oncol. 1996 Nov;32B(6):394-400. [DOI:10.1016/S0964-1955(96)00033-4]
14. Sargolzaei S, Farhadi S, Kazemi B, Bandehpour M, Kharazifard MJ. The correlation between p16 expression and INK4a locus mutation with grades and stages in oral squamous cell carcinoma. Indian J Pathol Microbiol. 2014 JanMar;57(1):24-30. [DOI:10.4103/0377-4929.130877] [PMID]
15. Dekker NP, Lozada-Nur F, Lagenaur LA, McPhail LA, Bloom CY, Regezi JA. Apoptosisassociated markers in oral lichen planus. J Oral Pathol Med. 1997 Apr;26(4):170-5. [DOI:10.1111/j.1600-0714.1997.tb00453.x] [PMID]
16. Al-Hashimi I, Schifter M, Lockhart PB, Wray D, Brennan M, Migliorati CA, et al. Oral lichen planus and oral lichenoid lesions: diagnostic and therapeutic considerations. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007 Mar;103 Suppl:S25.e1-12. [DOI:10.1016/j.tripleo.2006.11.001] [PMID]
17. Shahsavari F, Farhadi S, Sadri D, Sedehi M. Evaluation of Microvascularity by CD34 Expression in Esophagus and Oral Squamous Cell Carcinoma. J Contemp Dent Pract. 2015 Jun 1;16(6):458-62. [DOI:10.5005/jp-journals-10024-1706] [PMID]
18. Rödström PO , Jontell M, Mattsson U, Holmberg E. Cancer and oral lichen planus in a Swedish population. Oral Oncol. 2004 Feb;40(2):131-8. [DOI:10.1016/S1368-8375(03)00134-9]
19. Eisen D. The clinical features, malignant potential and systemic associations of oral lichen planus: a study of 723 patients. J Am Acad Dermatol. 2002 Feb;46(2):207-14. [DOI:10.1067/mjd.2002.120452] [PMID]
20. Mignogna MD, Lo Russo L, Fedele S. Gingival involvement of oral lichen planus in a series of 700 patients. J Clin Periodontol. 2005 Oct;32(10):1029-33. [DOI:10.1111/j.1600-051X.2004.00761.x] [PMID]
21. Wang D, Dubois RN. Prostaglandins and cancer. Gut. 2006 Jan;55(1):115-22. [DOI:10.1136/gut.2004.047100] [PMID] [PMCID]
22. Farhadi S, Ghorbanpour M, Pourabed F, Lotfi A. Vascular Endothelial Growth Factor Expression in Oral Lichen Planus: A Comparative Study. J Res Dentomaxillofac Sci. 2018;3(1):1-5. [DOI:10.29252/jrdms.3.1.1]
23. Nafarzadeh S, Jafari S, Bijani A. Assessment of bax and bcl-2 immunoexpression in patients with oral lichen planus and oral squamous cell carcinoma. Int J Mol Cell Med. 2013 Summer;2(3):136-42.
24. Stoicănescu D, Andreescu N, Belengeanu A, Meszaros N, Cornianu M. Assessment of p53 and HER-2/neu genes status and protein products in oral squamous cell carcinomas. Rom J Morphol Embryol. 2013 Jan 1;54(4):1107-13.
25. Crosthwaite N, Teale D, Franklin C, Foster GA, Stringer BM. p53 protein expression in malignant, pre-malignant and non-malignant lesions of the lip. J Clin Pathol. 1996 Aug;49(8):648-53. [DOI:10.1136/jcp.49.8.648] [PMID] [PMCID]
26. Warnakulasuriya KA, Johnson NW. Expression of p53 mutant nuclear phosphoprotein in oral carcinoma and potentially malignant oral lesions. J Oral Pathol Med. 1992 Oct;21(9):404-8. [DOI:10.1111/j.1600-0714.1992.tb01028.x] [PMID]
27. Schoelch ML, Le QT, Silverman S Jr, McMillan A, Dekker NP, Fu KK, et al. Apoptosisassociated proteins and the development of oral squamous cell carcinoma. Oral Oncol. 1999 Jan;35(1):77-85. [DOI:10.1016/S1368-8375(98)00065-7]
28. Stoll C, Baretton G, Ahrens C, Löhrs U. Prognostic significance of apoptosis and associated factors in oral squamous cell carcinoma. Virchows Arch. 2000 Feb;436(2):102-8. [DOI:10.1007/PL00008207] [PMID]
29. Kannan K, Latha PN, Shanmugam G. Expression of bcl-2 oncoprotein in Indian oral squamous cell carcinomas. Oral Oncol. 1998 Sep;34(5):373-6. [DOI:10.1016/S1368-8375(98)00037-2]
30. Sulkowska M, Famulski W, Chyczewski L, Sulkowski S. Evaluation of p53 and bcl-2 oncoprotein expression in precancerous lesions of the oral cavity. Neoplasma. 2001;48(2):94-8.
31. Teni T, Pawar S, Sanghvi V, Saranath D. Expression of bcl-2 and bax in chewing tobaccoinduced oral cancers and oral lesions from India. Pathol Oncol Res. 2002;8(2):109-14. [DOI:10.1007/BF03033719] [PMID]
32. Valente G, Pagano M, Carrozzo M, Carbone M, Bobba V, Palestro G, et al. Sequential immunohistochemical p53 expression in biopsies of oral lichen planus undergoing malignant evolution. J Oral Pathol Med. 2001 Mar;30(3):135-40. [DOI:10.1034/j.1600-0714.2001.300302.x] [PMID]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
