Volume 1, Issue 2 (Journal of Research in Dental & Maxillofacial Sciences spring 2016)                   J Res Dent Maxillofac Sci 2016, 1(2): 7-14 | Back to browse issues page


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Moini P, Eslami G, Taheri S, Valaei N, Naji Rad M. In Vitro Comparative Evaluation of the Antibacterial Efficacy of Licorice Aqueous Root Extract (Glycyrrhiza Glabra) and Chlorhexidine against Lactobacillus Acidophilus. J Res Dent Maxillofac Sci. 2016; 1 (2) :7-14
URL: http://jrdms.dentaliau.ac.ir/article-1-101-en.html
1- Assistant Professor, Pediatric Dept, Dental Branch of Tehran
2- Professor, Microbiology Dept, Shahid Beheshti University
3- Tutor, Microbiology Dept, Shahid Beheshti University
4- Faculty Member of Thalassemia Research Center
5- Dentist , najirad67@yahoo.com
Abstract:   (4076 Views)

Background and Aim: Oral infections and dental caries are considered as two serious public health problems, which inflict a costly burden on health care services worldwide, especially in the developing countries. The aim of this study was to evaluate the antibacterial activity of Iranian Licorice aqueous root extract on Lactobacillus Acidophilus in comparison with Chlorhexidine.

Materials and Methods: In this in-vitro experimental study, we evaluated the antibacterial activity of Licorice aqueous root extract and Chlorhexidine against Lactobacillus Acidophilus using Disk Diffusion Method, determining the Minimum Inhibitory Concentration (MIC) by Broth Micro & Macro Dilution Methods and the Minimum Bactericidal Concentration (MBC) by Agar Dilution Method. Research was repeated 3 times and data were analyzed by ANOVA test. The P value of ≤ 0.01 was considered as the level of significance.

Results: Chlorhexidine showed significantly higher levels of antibacterial activity against Lactobacillus Acidophilus in comparison with Licorice aqueous root extract (P < 0.01).

Conclusion: Although Licorice aqueous root extract is beneficial, Chlorhexidine is more efficient in the prevention of dental caries and oral infections.

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Type of Study: Original article | Subject: Radiology

References
1. Vyas YK, Bhatnagar M, Sharma K. In vitro evaluation of antibacterial activity of an herbal dentifrice against Streptococcus mutans and Lactobacillus acidophilus. Indian J Dent Res 2008;19 (1): 26-8.
2. Malhotra N, Rao SP, Acharya S, Vasudev B. Comparative in vitro evaluation of efficacy of mouthrinses against Streptococcus mutans, Lactobacilli and Candida albicans. Oral Health Prev Dent 2011; 9(3):261-8.
3. Subramaniam P, Nandan N. Effect of xylitol, sodium fluoride and triclosan containing mouth rinse on Streptococcus mutans. Contemp Clin Dent 2011; 2(4):287-90.
4. Messier C, Epifano F, Genovese S, Grenier D. Licorice and its potential beneficial effects in common oro‐dental diseases. Oral Dis 2012; 18(1):32-9.
5. Villinski JR , Bergeron C, Cannistra JC, Gloer JB, Coleman CM, Ferreira D, et al. Pyrano -isoflavones from glycyrrhiza uralensis with antibacterial activity against streptococcus mutans and porphyromonas gingivalis. J Nat Prod 2014; 28;77(3):521-6.
6. Hamdon SM , Ghada Y, Rahman A. Glycyrrhiza glabara as antibacterial agent on biofilm and planktonic cell of aggregatibacter actinomycetemcomitans. Int J Dent Sci Res 2014; (2) 42-46.
7. Asl MN, Hosseinzadeh H. Review of pharmacological effects of Glycyrrhiza sp. and its bioactive compounds. Phytother Res 2008;22(6):709-24.
8. Ajagannanavar SL, Battur H, Shamarao S, Sivakumar V, Patil PU, Shanavas P. Effect of aqueous and alcoholic licorice (glycyrrhiza glabra) root extract against streptococcus mutans and lactobacillus acidophilus in comparison to chlorhexidine: an in vitro study. J Int Oral Health 2014; 6(4):29-34.
9. Sedighinia F, Safipour Afshar A, Soleimanpour S, Zarif R, Asili J, Ghazvini K. Antibacterial activity of Glycyrrhiza glabra against oral pathogens: an in vitro study. Avicenna J Phytomed 2012; 2(3):118-24.
10. More G, Tshikalange TE, Lall N, Botha F, Meyer JJ. Antimicrobial activity of medicinal plants against oral microorganisms. J Ethnopharmacol 2008; 28;119(3): 473-7.
11. Ahn S-J, Song YD, Mah SJ, Cho EJ, Kook JK. Determination of optimal concentration of deglycyrrhizinated licorice root extract for preventing dental caries using a bacterial model system. Journal of Dental Sciences 2014;( 9):214-20.
12. Jayaprakash R, Sharma A, Moses J. Comparative evaluation of the efficacy of different concentrations of chlorhexidine mouth rinses in reducing the mutants streptococci in saliva: An in vivo study. J Indian Soc Pedod Prev Dent 2010;28(3):162-6.
13. Ahn SJ, Cho EJ, Kim HJ, Park SN, Lim YK, Kook JK. The antimicrobial effects of deglycyrrhizinated licorice root extract on Streptococcus mutans UA159 in both planktonic and biofilm cultures. Anaerobe 2012; 18(6):590-6.
14. Ahn SJ, Park SN, Lee YJ, Cho EJ, Lim YK, Li XM, et al. Invitro antimicrobial activities of 1-Methoxyficifolionol, Licorisoflavan A, and 6,8-Diprenylgenistein against streptococcus mutans. Caries Res 2015; 49(1):78-89.
15. Jain E, Pandey RK, Khanna R. Liquorice root extracts as potent cariostatic agents in pediatric practice. J Indian Soc Pedod Prev Dent 2013; 31(3):146-52.
16. Hosseini A, Shafiee-Nick R, Mousavi SH. Combination of Nigella sativa with Glycyrrhiza glabra and Zingiber officinale augments their protective effects on doxorubicin-induced toxicity in H9c2 cells. Iran J Basic Med Sci 2014; 17(12):993-1000.
17. Hu CH, He J, Eckert R, Wu XY, Li LN, Tian Y, et al. Development and evaluation of a safe and effective sugar-free herbal lollipop that kills cavity-causing bacteria. Int J Oral Sci 2011; 3(1): 13-20.

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