Volume 7, Issue 2 (3-2022)                   J Res Dent Maxillofac Sci 2022, 7(2): 91-97 | Back to browse issues page

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Moshari A, Esnaashari E, Eskandarion S, Safari B. Comparison of Cytotoxicity of AH Plus and DC Canal SE Sealer After Final Setting. J Res Dent Maxillofac Sci 2022; 7 (2) :91-97
URL: http://jrdms.dentaliau.ac.ir/article-1-315-en.html
Comparison of Cytotoxicity of AH Plus and DC Canal SE Sealer After Final Setting
AA Moshari * 1, E Esnaashari2 , S Eskandarion3 , B Safari4
1- Department of Endodontics, Dental Material Research Center, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , Amirabbas.moshari@gmail.com
2- Department of Endodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
3- Dental Material Research Center, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
4- Private Dentistry Practice, Tehran, Iran
Abstract:   (1503 Views)

Background and Aim: This study aimed to compare the cytotoxicity of AH Plus and DC Canal SE sealer after final setting.
Materials and Methods: In this in vitro, experimental study, human gingival fibroblasts (HGFs) were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 µg/mL streptomycin, and incubated at 37°C and 5% CO2. Cells at a density of 5000 cells/well were seeded in a 96-well plate for the methyl thiazolyl tetrazolium (MTT) assay. The cells were incubated with AH Plus and DC Canal SE sealers. Specimens of freshly mixed sealers were fabricated with 4 mm height and 10 mm diameter, and  incubated at 37°C for 24 hours for setting. Each specimen was incubated with 10 mL of diluted culture medium at 37°C and 5% CO2 for 24 hours. Finally, the solution was filtered using a 0.22-µm filter. Different dilutions (1, 1/2, 1/4, 1/8) were prepared, and cell viability was assessed at 24 and 72 hours by measuring the optical density of the solutions spectrophotometrically. Data were analyzed by ANOVA and Tukey’s test.
Results: Cell viability in presence of all concentrations of AH Plus and DC Canal SE was significantly lower at 72 hours compared with 24 hours (P<0.001). Cell viability in presence of AH Plus was significantly higher compared with DC Canal SE at all concentrations and time points.
Conclusion: Cell viability was higher in presence of AH Plus compared with DC Canal SE sealer in all concentrations and time points.

Keywords: Endodontics, Root Canal Obturation, Root Canal Filling Materials
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Type of Study: Original article | Subject: Oral medicine
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