Journal of Research in Dental
and Maxillofacial Sciences
Volume 11, Issue 2 (6-2026)
J Res Dent Maxillofac Sci 2026, 11(2): 136-147 |
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Ethics code: 0972/HRECC.FODM/IX/2024
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Rachmadhan F F, Kamadjaja D B, Al Fessi R. In Vitro Study of Sustained Release Potential and IGF/PDGF Profile of Lyophilized Decellularized Bovine Bone Scaffolds After Freeze-Dried Secretome Application. J Res Dent Maxillofac Sci 2026; 11 (2) :136-147
URL: http://jrdms.dentaliau.ac.ir/article-1-1266-en.html
URL: http://jrdms.dentaliau.ac.ir/article-1-1266-en.html
1- CClinical Medicine Magister Study Programme, Faculty of Dental Medicine, Universitas Airlangga, Surabaya 60132, Indonesia
2- Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya 60132, Indonesia ,david-b-k@fkg.unair.ac.id
3- Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya 60132, Indonesia
2- Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya 60132, Indonesia ,
3- Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya 60132, Indonesia
Abstract: (17 Views)
Background and Aim: Craniomaxillofacial bone defects are commonly reconstructed using bovine bone scaffolds, where stable growth factor release is critical for effective bone regeneration. This study investigated the release profile of insulin-like growth factor (IGF) and platelet-derived growth factor (PDGF) from deproteinized bovine bone mineral (DBBM), freeze-dried bovine bone (FDBB), and decellularized freeze-dried bovine bone (dc-FDBB) scaffolds following application of freeze-dried secretome.
Materials and Methods: In this in vitro experimental study, scaffolds were first immersed in secretome and incubated at 4°C for 24 hours to facilitate absorption, followed by an optional freeze-drying step. After rehydration, the release kinetics of IGF and PDGF was measured at 1, 8, 24, and 48 hours using ELISA. Comparisons were made with one-way ANOVA followed by the Tukey’s HSD test (alpha=0.05).
Results: FDBB scaffolds treated with freeze-dried secretome exhibited the highest and most sustained release of IGF over 48 hours compared to other groups (26.774±4.079 ng/mL; P<0.05); while PDGF release was generally lower in freeze-dried groups compared to controls (8.587±2.184 ng/mL; P=0.197).
Conclusion: The results showed that freeze-drying influences the release profile of IGF and PDGF and its sustainability from secretome-loaded bovine bone scaffolds, with potential implications for optimizing scaffold bioactivity in bone tissue engineering applications.
Materials and Methods: In this in vitro experimental study, scaffolds were first immersed in secretome and incubated at 4°C for 24 hours to facilitate absorption, followed by an optional freeze-drying step. After rehydration, the release kinetics of IGF and PDGF was measured at 1, 8, 24, and 48 hours using ELISA. Comparisons were made with one-way ANOVA followed by the Tukey’s HSD test (alpha=0.05).
Results: FDBB scaffolds treated with freeze-dried secretome exhibited the highest and most sustained release of IGF over 48 hours compared to other groups (26.774±4.079 ng/mL; P<0.05); while PDGF release was generally lower in freeze-dried groups compared to controls (8.587±2.184 ng/mL; P=0.197).
Conclusion: The results showed that freeze-drying influences the release profile of IGF and PDGF and its sustainability from secretome-loaded bovine bone scaffolds, with potential implications for optimizing scaffold bioactivity in bone tissue engineering applications.
Type of Study: Original article |
Subject:
Oral & maxillofacial surgery
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