:: Volume 1, Issue 3 (Journal of Research in Dental & Maxillofacial Sciences summer 2016) ::
J Res Dentomaxillofac Sci 2016, 1(3): 1-7 Back to browse issues page
In Vitro Comparison of the Effect of Ledermix® Paste and Green Tea Extract on the Concentration of Inflammatory Mediators
M Vatanpour1, A Falah Doust1, G Shojaee2, A Jamshidian *3, A Farasat4
1- Assistant Professor, Endodontics Dept, Dental Branch of Tehran, Islamic Azad University, Tehran, Iran, Islamic Azad University, Tehran, Iran
2- Post Graduate student, Endodontics Dept, Dental faculty,Qom university of medical sciences,Qom,Iran, Islamic Azad University, Tehran, Iran
3- Post Graduate student,Endodontics Dept, Dental Branch of Tehran, Islamic Azad University, Tehran, Iran. , jmdn.ali@gmail.com
4- Pharmaceutical Sciences Branch,Islamic Azad University,Tehran,Iran
Abstract:   (2834 Views)

Background and aim: The purpose of this study is to compare the effect of Ledermix® paste and green tea extract on the concentration of inflammatory mediators.

Materials and methods: In this in-vitro experimental study, first, the noncytotoxic concentrations of Ledermix® medicament and green tea extract were determined by MTT assay. Afterwards, the concentrations of IL-1β, IL-6, and TNF-α inflammatory mediators were assessed by ELISA test in the four experimental groups and also in the control group at different time intervals of 2, 24, and 48 hours and after 7 days. One-way analysis of variance (ANOVA) was used for statistical analysis of the obtained raw data followed by Tukey's post-hoc test for pair comparisons.

Results: the noncytotoxic concentration of green tea extract and Ledermix® paste equaled 12 µg/ml and 0.25 µg/ml, respectively. Time interval had no significant effect on the concentration of the mediators. Generally, both substances significantly decreased the concentration of IL-1β and IL-6 inflammatory mediators in comparison with the control group.

Conclusions: Green tea extract is less cytotoxic and more biocompatible than Ledermix® paste. Both Ledermix® paste and green tea extract expressed anti-inflammatory effects.

Keywords: Cytotoxicity, Green tea extract, Inflammatory mediators, Ledermix® paste
Full-Text [PDF 243 kb]   (1052 Downloads) |   |   Full-Text (HTML)  (44 Views)  
Type of Study: Original article |
* Corresponding Author Address: Islamic Azad University, Tehran, Iran.
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