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:: Volume 3, Issue 1 (Journal of Research in Dental & Maxillofacial Sciences winter 2018) ::
J Res Dentomaxillofac Sci 2018, 3(1): 1-5 Back to browse issues page
Vascular Endothelial Growth Factor Expression in Oral Lichen Planus: A Comparative Study
S Farhadi1, M Ghorbanpour1, F Pourabed *2, A Lotfi3
1- Assistant Professor, Oral & Maxillofacial Pathology Dept, Islamic Azad University_ Tehran Dental Branch ,Tehran, Iran, Dental Branch of Tehran, Islamic Azad University, Tehran, Iran
2- Dental Branch of Tehran, Islamic Azad University, Tehran, Iran , golshid.p2015@gmail.com
3- Assistant professor, Oral & Maxillofactial Pathology dept,Dental faculty, Shahid Beheshti University of Madical Sciences, Tehran, Iran., Shahid Beheshti University of Madical Sciences, Tehran, Iran.
Abstract:   (867 Views)
Background and aim: Lichen planus (LP) is a chronic inflammatory disease with an uncertain pathogenicity. Recent reports have revealed that angiogenesis is effective in the pathogenesis and biologic behavior of lesions. Considering the limitations of the studies related to angiogenesis in oral LP (OLP) and the controversial results, the present study aimed to compare the expression of vascular endothelial growth factor (VEGF) in OLP and normal mucosa.
Materials and methods: In this case-control study, 4-µm sections were prepared from selected OLP (erosive type) blocks and normal mucosa samples for immunohistochemistry (IHC) staining with VEGF marker. VEGF expression was quantitatively assessed via counting the positive-stained cells. The mean percentages of positive-stained cells in each sample were categorized in three groups: score 0 (the presence of 10% or lower), score 1 (the presence of 10% to 50%), and score 2 (the presence of more than 50% of stained cells). T-test and Mann-U-Whitney test were used for statistical analyses.
Results: The mean percentage of VEGF expression was 39.8% in 35 OLP samples, and 20.5% in 20 normal mucosa samples. Twenty OLP samples (57%) had score 1, and 15 (43%) had score 2. Eighteen normal mucosa samples (90%) had score 1, and two (10%) had score 2. Also, score 0 was not reported in any of the samples. The percentages of VEGF expression in OLP and normal mucosa did not show any statistically significant differences (P=0.5). However, there was a significant difference between OLP and normal mucosa in VEGF expression score (P=0.012), and OLP samples had significantly higher scores.
Conclusion: According to the results, VEGF expression in erosive OLP samples was significantly higher than that in normal mucosa.
Keywords: Oral Lichen Planus, Vascular Endothelial Growth Factor, Immunohistochemistry, Angiogenesis
Full-Text [PDF 173 kb]   (209 Downloads) |   |   Full-Text (HTML)  (12 Views)  
Type of Study: Original article |
* Corresponding Author Address: Dental Branch of Tehran, Islamic Azad University, Tehran, Iran
1. Carmeliet P. Angiogenesis in health and disease. Nat Med. 2003 Jun;9(6):653-60.
2. Liekens S, De Clercq E, Neyts J. Angiogenesis: regulators and clinical applications. Biochem Pharmacol. 2001 Feb 1;61(3):253-70.
3. Seifi S, Shafaie S, Ghadiri S. Microvessel density in follicular cysts, keratocystic odontogenic tumors and ameloblastomas. Asian Pac J Cancer Prev. 2011;12(2):351-6.
4. Shahsavari F, Farhadi S, Sadri D, Sedehi M. Evaluation of Microvascularity by CD34 Expression in Esophagus and Oral Squamous Cell Carcinoma. J Contemp Dent Pract. 2015 Jun 1;16(6):458-62.
5. Sadri D, Shahsavari F, Farhadi S, Shahabi Z, Mehran Z. Angiogenesis concept in odontogenic keratocyst: A comparative study. Indian J Dent Res. 2017 May-Jun;28(3):275-80.
6. Sargolzaei S, Farhadi S, Kazemi B, Bandehpour M, Kharazifard MJ. The correlation between p16 expression and INK4a locus mutation with grades and stages in oral squamous cell carcinoma. Indian J Pathol Microbiol. 2014 Jan-Mar;57(1):24-30.
7. Folkman J. Angiogenesis in cancer, vascular, rheumatoid and other disease. Nat Med. 1995 Jan;1(1):27-31.
8. Brown LF, Detmar M, Claffey K, Nagy JA, Feng D, Dvorak AM, et al. Vascular permeability factor/vascular endothelial growth factor: a multifunctional angiogenic cytokine. EXS. 1997;79:233-69.
9. Ferrara N, Davis-Smyth T. The biology of vascular endothelial growth factor. Endocr Rev. 1997 Feb;18(1):4-25.
10. Leonardi R, Caltabiano M, Pagano M, Pezzuto V, Loreto C, Palestro G. Detection of vascular endothelial growth factor/ vascular permeability factor in periapical lesions. J Ended. 2003 Mar;29(3):180-3.
11. Sadri D, Farhadi S, Shahabi Z, Sarshar S. Expression of Vascular Endothelial Growth Factor in Odontogenic Cysts: Is There Any Impression on Clinical Outcome? Open Dent J. 2016 Dec 30;10:752-9.
12. Mitrou GK, Tosios KI, Kyroudi A, Sklavounou A. Odontogenic keratocyst expression vascular endothelial growth factor: an immunohistochemical study. J Oral Pathol Med. 2009 May;38(5):470-5.
13. Cooper ME, Vranes D, Youssef S, Stacker SA, Cox AJ, Rizkalla B, et al. Increased renal expression of vascular endothelial growth factor (VEGF) and its receptor VEGFR-2 in experimental diabetes. Diabetes. 1999 Nov;48(11):2229-39.
14. Tampa M, Caruntu C, Mitran M, Mitran C, Sarbu I, Rusu LC,et al. Markers of Oral Lichen Planus Malignant Transformation. Dis Markers. 2018 Feb 26;2018:1959506.
15. Mittal N, Shankari GM, Palaskar S. Role of angiogenesis in the pathogenesis of oral lichen planus. J Oral Maxillofac Pathol. 2012 Jan;16(1):45-8.
16. Scardina GA, Ruggieri A, Messina P, Maresi E. Angiogenesis of oral lichen planus: a possible pathogenetic mechanism. Med Oral Patol Oral Cir Bucal. 2009 Nov 1;14(11):e558-62.
17. Rubini C, Artese L, Zizzi A, Fioroni M, Ascani G, Goteri G, et al. Immunohistochemical expression of vascular endothelial growth factor (VEGF) in different types of odontogenic cysts. Clin Oral Investig. 2011 Oct;15(5):757-61.
18. Tao X, Huang Y, Li R, Qing R, Ma L, Rhodus NL, et al. Assessment of local angiogenesis and vascular endothelial growth factor in patients with atrophic-erosive and reticular oral lichen planus. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007 May;103(5):661-9.
19. Ding M, Xu JY, Fan Y. Altered expression of mRNA for HIF-1alpha and its target genes RTP80I and VEGF in patients with oral lichen planus. Oral Dis. 2010 Apr;16(3):299-304.
20. Výbohová D, Mellová Y, Adamicová K, Adamkov M, Hešková G. Quantitative immunohistochemical assessment of blood and lymphatic microcirculation in cutaneous lichen planus lesions. Histol Histopathol. 2015 Jun;30(6):697-706.
21. Mardani M, Ghabanchi J, Fattahi MJ, Tadbir AA. Serum level of vascular endothelial growth factor in patients with different clinical subtypes of oral lichen planus. Iran J Med Sci. 2012 Dec;37(4):233-7.
22. Ferrara N, Gerber HP, LeCouter J. The biology of VEGF and its receptors. Nat Med. 2003 Jun;9(6):669-76.
23. Cornelini R, Artese L, Rubini C, Fioroni M, Ferrero G, Santinelli A, et al. Vascular endothelial growth factor and microvessel density around healthy and failing dental implants. Int J Oral Maxillofac Implants. 2001 May-Jun;16(3):389-93.
24. Creamer D, Sullivan D, Bicknell R, Barker J. Angiogenesis in psoriasis. Angiogenesis. 2002;5(4):231-6.
25. Bonnet CS, Walsh DA. Osteoarthritis, angiogenesis and inflammation. Rheumatology (Oxford). 2005 Jan;44(1):7-16.
26. Szekanecz Z, Besenyei T, Paragh G, Koch AE. Angiogenesis in rheumatoid arthritis. Autoimmunity. 2009 Nov;42(7):563-73.
27. Kirk S, Frank JA, Karlik S. Angiogenesis in multiple sclerosis: is it good, bad or an epiphenomenon? J Neurol Sci. 2004 Feb 15;217(2):125-30.
28. Watanabe H, Mamelak AJ, Wang B, Howell BG, Freed I, Esche C, et al. Anti-vascular endothelial growth factor receptor-2 (FlK-1/KDR) antibody suppresses contact hypersensitivity. Exp Dermatol. 2004 Nov;13(11):671-81.
29. Koutroubakis IE, Xiadakis C, Karmiris K, Sfiridaki A, Kandidaki E, Kouroumalis EA. Serum angiogenin in inflammatory bowel disease. Dig Dis Sci. 2004 Nov-Dec;49(11-12):1758-62.
30. Scardina GA, Picone V, Cacioppo A, Messina P. Study of microcirculation in oral lichen planus by video-capillaroscopy. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007 Apr;103(4):e30-4.
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Farhadi S, Ghorbanpour M, Pourabed F, Lotfi A. Vascular Endothelial Growth Factor Expression in Oral Lichen Planus: A Comparative Study. J Res Dentomaxillofac Sci. 2018; 3 (1) :1-5
URL: http://jrdms.dentaliau.ac.ir/article-1-192-en.html

Volume 3, Issue 1 (Journal of Research in Dental & Maxillofacial Sciences winter 2018) Back to browse issues page
Journal of Research in Dental and Maxillofacial Sciences
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