Volume 1, Issue 3 (7-2016)                   J Res Dent Maxillofac Sci 2016, 1(3): 28-32 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Azizi A, Dabirmoghadam P, Keykha F. Comparison of the Concentration of Salivary IL-8 in Patients with Oral Squamous Cell Carcinoma and Healthy Subjects. J Res Dent Maxillofac Sci 2016; 1 (3) :28-32
URL: http://jrdms.dentaliau.ac.ir/article-1-128-en.html
Comparison of the Concentration of Salivary IL-8 in Patients with Oral Squamous Cell Carcinoma and Healthy Subjects
A Azizi * 1, P Dabirmoghadam2 , F Keykha3
1- Professor, Oral Medicine Dept, Dental Branch of Tehran, Islamic Azad University,Tehran, Iran , drarashazizi@yahoo.com
2- Assistant professor, Otolaryngology Research center, Amiralam Hospital, Tehran University of Medical Sciences, Tehran, IranTehran University of Medical Sciences, Tehran, Iran
3- Tehran
Abstract:   (5661 Views)

Background and aim: oral cancer is one of the most common cancers of the head and neck. Considering the high prevalence and mortality percentage of oral cancer and the significant advances in immunology and identification of different cytokines, the aim of the present study was to compare the concentration of IL8 in saliva of patients with oral squamous cell carcinoma (OSCC) and healthy subjects.

Materials and methods: in this case-control study, 20 individuals with a definite histopathology diagnosis of OSCC were selected as the case group and 20 healthy subjects were selected as the control group. The two groups were matched according to age, gender, cigarette smoking and alcohol consumption, medication consumption, periodontal disease (average CAL in first molars) and absence of systemic diseases. 5 ml of unstimulated whole saliva sample was collected with spitting method from each subject and the concentrations of salivary IL8 were compared between the two groups. Linear regression and t-test were used for statistical analysis.

Results: the concentration of IL8 equaled 741.82±722.81 pg/ml in the case group and 341.25±313.52 pg/ml in the control group and Mann-U-Whitney test showed that the concentration of IL8 in patients with OSCC is higher than that in the control subjects and this difference is significant. (p=0.049) According to regression equation, IL8 has a significant correlation with tumor size index (T). (p=0.011)

Conclusion: the results of this study showed that the concentrations of salivary IL8 were significantly different between the patients with OSCC and healthy subjects (p=0.049) and the concentration of IL8 has a significant correlation with tumor size (T).

Keywords: Interleukin 8, oral squamous cell carcinoma, saliva
Full-Text [PDF 313 kb]   (1571 Downloads) |   |   Full-Text (HTML)  (719 Views)  
Type of Study: Original article | Subject: Oral medicine
References
1. Tomikoshi Y, Nomura M,Okudaira N,Sakagami H,Wakabayashi H.Enhancement of cytotoxicity of three Apoptosis-inducing agents against human oral squamous cell carcinoma cell line by Benzoxazinotropone. In Vivo 2016;30(5):645-50.
2. Li Y,Cui JT.Inhibition of Bcl-2 potentiates AZD-2014 induced anti-head and neck squamous cell carcinomacell activity. Biochem Biophys Res Commun 2016 ;477(4);607-13.
3. Terato K,Do C,Chang J, Waritani T.Preventing further minuse of the elisa technique and misinterpretation of serological antibody assay data. Vaccine 2016;34(39):4643-4.
4. Martin JL, Gottehrer N, Zalesin H,Hoff PT,Shaw M,Clarkson JH and et al.Evaluation of salivary transcriptome markers for the early detection of oral squamous cell cancer in a prospective blinded trial. Compend contin educ Dent 2015;36(5):365-73.
5. Sahebjamee M, Eslami M, AtarbashiMoghadam F, Sarafinejad A. Salivary concentration of TNF alpha ,IL1 alpha,IL6,and IL8 in oral squamous cell carcinoma. Med Oral Patol Oral Cir Bucal 2008;13(5):E292-5.
6. Osman TA,Costea DE,Johannessen AC. The use of salivary cytokines as a screening tool for oral squamous cell carcinoma: Areview of the literature. J Oral Maxillofac Pathol 2012;16(2);256-61.
7. Ward MJ,Mellows T,Harris S,Webb A,Patel NN,Cox HJ and et al.Staging and treatment of oropharyngeal cancer in the human papillomavirus era. Head Neck 2015;37(7):1002-13.
8. Azizi A,Rezaei M.Prevalence of candida species in the oral cavity of patients undergoing head and neck radiotherapy. J Dent Res Dent Clin Dent Prospects 2009;3(3):78-81.
9. Sadri D,Azizi A,Farhadi S, Shokrgozar H, Entezari N .Head and neck metastatic tumors:a retrospective survey of Iranian patients .J Dent(shiraz) 2015;16(1):17-21.
10. 10 –Su KJ,Lin CW,Chen MK,Yang SF.YU YL.Effects of EZH2 promoter polymorphisms and methylation status on oral squamous cell carcinoma susceptibility and pathology.Am J Cancer Res 2015;5(11):3475-84.
11. Murphy PM,Tiffany HL. Cloning of complementary DNA encoding a functional human interleukin-8 receptor. Science .1991.253:1280-1283. J Immunol. 2009; 183(5):2898-901.
12. Yang CY,Brooks E,Li Y,Denny P,Ho CM,Qi F and et al. Detection of picomolar levels of interleukin-8 in human saliva by SPR. Lab Chip 2005;5(10):1017-23.
13. Allin KH,Bojesen SE,Nordestgaard BG.Inflammatory biomarkers and risk of cancer in 84,000 individuals from the general population. Int J Cancer 2016;139(7):1493-500.
14. Azizi A,Ranjbari A,Ghafari MA,Jahan F. Comparative evaluation of lactate dehydrogenase(LDH) and aspartate aminotransferase(AST)levels in periodontal disease.J Isfahan Dent Sch2011;7(3):271-77
15. Azizi A,Lawaf S.The management of oral pemphigus vulgaris with systemic corticosteroid and dapsone.J Dent Res Dent Clin Dent Prospects2008;2(1):33-7.
16. Aggarwal BB, Gehlot P. Inflammation and cancer: how friendly is the relationship For cancer patients? Curr Opin Pharmacol 2009; 9(4): 351-69.
17. Brat DJ, Bellail AC, Van Meir EG. The role of interleukin-8 and its receptors in gliomagenesis and tumoral angiogenesis. Neuro oncol 2005;7(2): 122-133.
18. Azizi A, Lawaf S. The comparison of efficacy of adcortyl ointment and topical tacrolimus I treatment of erosive oral lichen planus. J Dent Res Dent Clin Dent Prospects 2007;1(3):99-102.
19. Boxman I L, Ruwhof C, Boerman OC, Löwik C W, Ponec M. Role of fibroblasts in the regulation of proinflammatory interleukin IL-1, IL-6 and IL-8 levels induced by keratinocyte-derived IL-1. Arch Dermatol Res 1996;288(7): 391-8.
20. Dentener MA, Bazil V, Von Asmuth EJ, Ceska M, Buurman WA. Involvement of CD14 in lipopolysaccharide-induced tumor necrosis factor-alpha, IL-6 and IL-8 release by human monocytes and alveolar macrophages. J Immunol 1993:150(7);2885-91.
21. Punyani SR ,Sathawane RS: Salivary level of interleukin-8 in oral precancer and squamous cell carcinoma. Clin Oral investig 2013:17(2);517-24.
Add your comments about this article : Your username or Email:
CAPTCHA
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Research in Dental and Maxillofacial Sciences

Designed & Developed by: Yektaweb