دوره 8، شماره 1 - ( 10-1401 )
جلد 8 شماره 1 صفحات 27-18 |
برگشت به فهرست نسخه ها
Ethics code: IR.IAU.DENTAL.REC.1399.126
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Tavazo Zadeh E, Aref P, Askarizadeh N, Emadi F. In vitro Antimicrobial Effect of Punica granatum Extract versus Chlorhexidine on Streptococcus sobrinus, Streptococcus sanguinis, and Candida albicans. J Res Dent Maxillofac Sci 2023; 8 (1) :18-27
URL: http://jrdms.dentaliau.ac.ir/article-1-395-fa.html
URL: http://jrdms.dentaliau.ac.ir/article-1-395-fa.html
In vitro Antimicrobial Effect of Punica granatum Extract versus Chlorhexidine on Streptococcus sobrinus, Streptococcus sanguinis, and Candida albicans. . 1401; 8 (1) :18-27
چکیده: (834 مشاهده)
Background and Aim: Considering the search for an effective antimicrobial agent comparable to chlorhexidine (CHX), this study aimed to assess the antimicrobial effect of Punica granatum (P. granatum) hydroalcoholic extract on Streptococcus sobrinus (S. sobrinus), Streptococcus sanguinis (S. sanguinis) and Candida albicans (C. albicans), in comparison with CHX.
Materials and Methods: In this in vitro study, the disc diffusion test was used to assess the antimicrobial activity of the extract by measuring the growth inhibition zones; while, the microdilution and macrodilution broth tests were applied to find the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract against the tested microorganisms. The MBC was measured using the blood agar or Mueller Hinton agar culture medium. The Sabouraud dextrose agar culture medium was used for C. albicans. Each test was repeated in triplicate, and data were analyzed by independent samples t-test and Mann-Whitney U test.
Results: None of the tested microorganisms showed any resistance to the extract. CHX had the highest antimicrobial effect against all tested microorganisms. The MIC of the hydroalcoholic extract of P. granatum was 2.5 mg/mL for S. sobrinus and S. sanguinis, and 5 mg/mL for C. albicans. Its MBC was 5 mg/mL for S. sobrinus and S. sanguinis, and 10 mg/mL for C. albicans. The mean diameter of the growth inhibition zone for S. sobrinus caused by CHX was significantly greater than that caused by P. granatum extract (Mann-Whitney U test, P=0.043). The same result was obtained for S. sanguinis (Student sample t-test, P=0.002), and C. albicans (Mann-Whitney U test, P=0.046).
Conclusion: The hydroalcoholic extract of P. granatum has bacteriostatic and bactericidal effects on S. sanguinis and S. sobrinus and antifungal effect on C. albicans comparable to CHX.
Materials and Methods: In this in vitro study, the disc diffusion test was used to assess the antimicrobial activity of the extract by measuring the growth inhibition zones; while, the microdilution and macrodilution broth tests were applied to find the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract against the tested microorganisms. The MBC was measured using the blood agar or Mueller Hinton agar culture medium. The Sabouraud dextrose agar culture medium was used for C. albicans. Each test was repeated in triplicate, and data were analyzed by independent samples t-test and Mann-Whitney U test.
Results: None of the tested microorganisms showed any resistance to the extract. CHX had the highest antimicrobial effect against all tested microorganisms. The MIC of the hydroalcoholic extract of P. granatum was 2.5 mg/mL for S. sobrinus and S. sanguinis, and 5 mg/mL for C. albicans. Its MBC was 5 mg/mL for S. sobrinus and S. sanguinis, and 10 mg/mL for C. albicans. The mean diameter of the growth inhibition zone for S. sobrinus caused by CHX was significantly greater than that caused by P. granatum extract (Mann-Whitney U test, P=0.043). The same result was obtained for S. sanguinis (Student sample t-test, P=0.002), and C. albicans (Mann-Whitney U test, P=0.046).
Conclusion: The hydroalcoholic extract of P. granatum has bacteriostatic and bactericidal effects on S. sanguinis and S. sobrinus and antifungal effect on C. albicans comparable to CHX.
نوع مطالعه: Original article |
موضوع مقاله:
Restorative Dentistry
| بازنشر اطلاعات | |
 |
این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است. |
