en Endodontics Endodontics Original article Original article <div style="text-align: justify;"><span style="font-size:13pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:105%"><span style="font-family:" verdana=""><span style="color:#2e74b5">Background and Aim</span></span></span></span></b><b><span style="font-size:9.0pt"><span style="line-height:105%"><span style="font-family:" verdana=""><span style="color:#2e74b5">:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="line-height:105%"><span style="font-family:" verdana="">This study aimed to assess the effect of 660 nm and 808 nm low-level diode laser irradiation on the production of vascular endothelial growth factor (VEGF) by human gingival fibroblasts (HGFs). &nbsp;</span></span></span>&nbsp;</span></span></span><br> <span style="font-size:13pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:105%"><span style="font-family:" verdana=""><span style="color:#2e74b5">Materials and Methods: </span></span></span></span></b><span style="font-size:9.0pt"><span style="line-height:105%"><span style="font-family:" verdana="">In this in vitro experimental study, HGFs were cultured in McCoy&rsquo;s 5A modified medium and assigned to three groups of control, 660 nm laser irradiation, and 808 nm laser irradiation. The concentration of VEGF was measured in the three groups at 1, 3, and 7 days after the intervention using ELISA. The three groups were compared in this respect using repeated measures ANOVA. Statistical significance was defined as P<0.05. </span></span></span>&nbsp;&nbsp;</span></span></span><br> <span style="font-size:13pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:105%"><span style="font-family:" verdana=""><span style="color:#2e74b5">Results: </span></span></span></span></b><span style="font-size:9.0pt"><span style="line-height:105%"><span style="font-family:" verdana=""><span style="color:#0e101a">The concentration of VEGF in 808 nm laser group was significantly higher than that in the control and 660 nm laser groups (P<0.05). Also, the concentration of VEGF in 660 nm laser group was significantly higher than that in the control group (P<0.05). The concentration of VEGF at 7 days was significantly higher than that at 1 and 3 days, and the value at 3 days was significantly higher than that at 1 day in all three groups (P<0.05).</span></span></span></span><span style="font-size:9.0pt"><span style="line-height:105%"><span style="font-family:"Verdana",sans-serif"></span></span></span></span></span></span><br> <span style="font-size:13pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:105%"><span style="font-family:" verdana=""><span style="color:#2e74b5">Conclusion:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="line-height:105%"><span style="font-family:" verdana=""><span style="color:#0e101a">Within the limitations of this study, it appears that irradiation of 660 and 808 nm diode laser can increase the production of VEGF by HGFs.</span></span></span></span></span></span></span></div> Fibroblasts, Low-Level Light Therapy, Vascular Endothelial Growth Factors 82 87 http://jrdms.dentaliau.ac.ir/browse.php?a_code=A-10-1773-1&slc_lang=en&sid=1 Mahta Fazlyab 100319475328460014821 100319475328460014821 No Department of Endodontics, TeMS.C., Islamic Azad University, Tehran, Iran Zohreh Sadat Hosseini 100319475328460014822 100319475328460014822 No Private Dentist, Tehran, Iran. Mehdi Vatanpour 100319475328460014823 100319475328460014823 No Department of Endodontics, TeMS.C., Islamic Azad University, Tehran, Iran Fatemeh Rezaei dr.fatemeh.rezaeii@gmail.com 100319475328460014824 100319475328460014824 Yes Department of Endodontics, TeMS.C., Islamic Azad University, Tehran, Iran