Journal of Research in Dental and Maxillofacial Sciences

en Effect of Chlorhexidine Application on Microleakage of Pit and Fissure Sealant Therapy with Self-Etch and Etch-And-Rinse Adhesives: An in Vitro Study Restorative Dentistry Restorative Dentistry Original article Original article <div style="text-align: justify;">Background and Aim: Application of disinfectants in pits and fissures is a commonly used method to lower the cariogenic bacterial count in teeth. This study compared the effect of applying chlorhexidine on microleakage of pit and fissure sealant therapy using two adhesive systems. Materials and Methods: In this in vitro study, 60 extracted molar teeth were randomly assigned to four groups (n=15): control group 1 (etch-and-rinse adhesive), control group 2 (self-etch adhesive), group 3 (etch-and-rinse adhesive and 0.2% chlorhexidine), and group 4 (self-etch adhesive and 0.2% chlorhexidine). After applying the sealant and subjecting the teeth to thermocycling, the teeth were immersed in 5% methylene blue solution. The teeth were sectioned into four parts and the dye penetration depth was measured under a stereomicroscope. Data were analyzed by the Mann–Whitney and Kruskal–Wallis tests using SPSS 24 (alpha=0.05).  Results: Microleakage was significantly lower in the self-etch adhesive system compared to the etch-and-rinse system in the control groups (P<0.05). For the etch-and-rinse adhesive, there was no significant difference in microleakage between the two groups with chlorhexidine (1.67±1.11) and without chlorhexidine (1.33±0.81) (P>0.05). Similarly, in the self-etch adhesive, microleakage did not significantly differ between groups with chlorhexidine (1.07±0.45) and without chlorhexidine (1.07±0.70) (P>0.05). The interaction effect of adhesive type and chlorhexidine on microleakage was not significant (P=0.428). Conclusion: Application of chlorhexidine with both self-etch and etch-and-rinse adhesive systems did not significantly affect the microleakage of fissure sealant therapy. Chlorhexidine may be used under fissure sealants for its antimicrobial effects without increasing microleakage.  </div> Chlorhexidine, Dental Leakage, Dentin-Bonding Agents, Pit and Fissure Sealants 250 256 http://jrdms.dentaliau.ac.ir/browse.php?a_code=A-10-1436-1&slc_lang=en&sid=1 Yashar Rezaei dr.yrezaei@yahoo.com 100319475328460013375 100319475328460013375 No Department of Dental Biomaterials, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran Anahita Ahmad Pour Zabarjad anahita.ahmadpourz@gmail.com 100319475328460013376 100319475328460013376 No Private Dental Practice, Tabriz, Iran Katayoun Katebi k_katebi@yahoo.co.uk 100319475328460013377 100319475328460013377 Yes Department of Oral and Maxillofacial Medicine, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran Sasan Bagheri Maleki sasansbm@gmail.com 100319475328460013378 100319475328460013378 No Private Dental Practice, Tabriz, Iran