en Endodontics Endodontics Original article Original article <div> <table align="center" hspace="0" vspace="0"> <tbody> <tr> <td style="padding: 0cm 12px; text-align: justify;" valign="top"><span style="font-size:13pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="font-family:" verdana=""><span style="color:#2e74b5">Background and Aim</span></span></span></b><b><span style="font-size:9.0pt"><span style="font-family:" verdana=""><span style="color:#2e74b5">:</span></span></span></b><b> </b><span style="font-size:9.0pt"><span style="font-family:" verdana="">This study aimed to assess the effect of calcium hydroxide (CH) in combination with betamethasone or ciprofloxacin on <i>Enterococcus faecalis </i>(<i>E. faecalis</i>) biofilm. &nbsp;</span></span></span></span><br> <span style="font-size:13pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="font-family:" verdana=""><span style="color:#2e74b5">Materials and Methods: </span></span></span></b><span style="font-size:9.0pt"><span style="font-family:" verdana="">This <i>ex vivo </i>experimental study was conducted on 95 single-rooted human teeth. The root canals were prepared and inoculated with <i>E. faecalis</i>. The samples were incubated for 8 weeks, and biofilm formation was confirmed by observation of 2 samples under a scanning electron microscope (SEM) and culture in 3 samples. The remaining samples were divided into three groups (n=30): CH + betamethasone eye drop, CH + ciprofloxacin eye drop, and CH + saline. Microbial analysis was performed at 1, 7 and 10 days. The colony count was measured. Comparisons were made by two-way ANOVA and a post-hoc test using SPSS.</span></span></span></span><br> <span style="font-size:13pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="font-family:" verdana=""><span style="color:#2e74b5">Results:</span></span></span></b><b> </b><span style="font-size:9.0pt"><span style="font-family:" verdana="">On day 1, the highest colony count was observed in CH + saline group. The difference in colony count between CH + ciprofloxacin and CH + betamethasone, and the difference between CH + betamethasone and CH + saline were not significant at days 7 and 10. CH + ciprofloxacin showed significantly lower colony count than CH + saline at 1 (P=0.001), 7 (P=0.004) and 10 (P=0.002) days.</span></span></span></span><br> <span style="font-size:13pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="font-family:" verdana=""><span style="color:#2e74b5">Conclusion:</span></span></span></b> <span style="font-size:9.0pt"><span style="font-family:" verdana="">The result of this study showed that betamethasone and ciprofloxacin were appropriate carriers for CH and resulted in suitable antibacterial effects against <i>E. faecalis</i>. </span></span></span></span></td> </tr> </tbody> </table> </div> <div style="text-align: justify;"></div> Betamethasone, Biofilms, Calcium Hydroxide, Ciprofloxacin, Enterococcus faecalis 179 186 http://jrdms.dentaliau.ac.ir/browse.php?a_code=A-10-1100-1&slc_lang=en&sid=1 Saman Amini s.amini755@yahoo.com 100319475328460012011 0000000304656835 No Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Mahta Fazlyab Dr.Mfazlyab@gmail.com 100319475328460012012 0000000301862908 No Department of Endodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Amir Abbas Moshari amirabbas.moshari@gmail.com 100319475328460012013 0000000334827500 No Department of Endodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Nika Soltani soltaninika@yahoo.com 100319475328460012014 0000000252139966 Yes Department of Endodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran