en Endodontics Endodontics Original article Original article <div> <table align="center" hspace="0" vspace="0"> <tbody> <tr> <td style="padding: 0cm 12px; text-align: justify;" valign="top"><span style="font-size:13pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="font-family:" verdana=""><span style="color:#2e74b5">Background and Aim</span></span></span></b><b><span style="font-size:9.0pt"><span style="font-family:" verdana=""><span style="color:#2e74b5">:</span></span></span></b><b> </b><span style="font-size:9.0pt"><span style="font-family:" verdana="">The main goal of root canal treatment is to three-dimensionally seal the root canal system. Since sealer may contact the periapical tissue, it should be biocompatible and safe for the body. This study aimed to assess the cytotoxicity of MTA Fillapex and Endoseal MTA calcium silicate-based sealers and AH Plus resin-based sealer for human gingival fibroblasts (HGFs). &nbsp;&nbsp;</span></span></span></span><br> <span style="font-size:13pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="font-family:" verdana=""><span style="color:#2e74b5">Materials and Methods:</span></span></span></b> &nbsp;<span style="font-size:9.0pt"><span style="font-family:" verdana="">In this in vitro study, extracts of AH Plus, Endoseal MTA, and MTA Fillapex were obtained, serially diluted 1:2, 1:4, and 1:8, and were exposed to HGFs. Cytotoxicity was assessed by the methyl thiazolyl tetrazolium (MTT) assay. Data were analyzed by ANOVA and Tukey&rsquo;s test (alpha=0.05). </span></span></span></span><br> <span style="font-size:13pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="font-family:" verdana=""><span style="color:#2e74b5">Results:</span></span></span></b><span style="font-size:9.0pt"><span style="font-family:" verdana=""> No significant difference existed between AH Plus 1:2 and MTA Fillapex 1:2 concentrations regarding the cell viability percentage (P>0.05). However, the difference between these two sealers in other concentrations was significant (P<0.05). No significant difference existed in AH Pus 1:4, Endoseal MTA 1:2, and MTA Fillapex 1:2 in cell viability (P>0.05); however, the difference among other concentrations of the three sealers was significant (P<0.05). The difference among AH Plus 1:8, Endoseal MTA 1:4, and MTA Fillapex 1:4 and 1:8 concentrations was not significant (P>0.05) but the difference among other concentrations was significant (P<0.05). Endoseal MTA 1:8 showed the highest and AH Plus 1:2 showed the lowest cell viability. </span></span></span></span><br> <span style="font-size:13pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="font-family:" verdana=""><span style="color:#2e74b5">Conclusion:</span></span></span></b> <span style="font-size:9.0pt"><span style="font-family:" verdana="">Endoseal MTA in all concentrations had lower cytotoxicity than MTA Fillapex and AH Plus and resulted in higher viability of HGFs. </span></span></span></span></td> </tr> </tbody> </table> </div> <div style="text-align: justify;"></div> Root Canal Obturation, Calcium Compounds, Epoxy Resins, Dental Cements, Fibroblast 203 209 http://jrdms.dentaliau.ac.ir/browse.php?a_code=A-10-391-6&slc_lang=en&sid=1 Narjes Hoshyari Narjeshoshyari@rocketmail.com 100319475328460012024 0000000226289290 Yes Department of Endodontics, School of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran Abbas Mesgarani a_mesgarani@yahoo.com 100319475328460012025 100319475328460012025 No Department of Endodontics, School of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran Mohammad Shokrzadeh Lamuki mslamuk@yahoo.com 100319475328460012026 100319475328460012026 No Department of Pharmacology and Toxicology, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Farzane Motafeghi Farzaneh.motafeghi@gmail.com 100319475328460012027 100319475328460012027 No Department of Pharmacology and Toxicology, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Seyed Jaber Mousavi j_mousavi@mazums.ac.ir 100319475328460012028 100319475328460012028 No Department of Community Medicine, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Milad Vaghar Mousavi milad.vmusavi@gmail.com 100319475328460012029 100319475328460012029 No Private Dental Practice, Sari, Iran