en Oral medicine Oral medicine Original article Original article <div> <table align="center" hspace="0" vspace="0"> <tbody> <tr> <td style="padding: 0cm 12px; text-align: justify;" valign="top"><span style="font-size:13pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="font-family:" verdana=""><span style="color:#2e74b5">Background and Aim</span></span></span></b><b><span style="font-size:9.0pt"><span style="font-family:" verdana=""><span style="color:#2e74b5">:</span></span></span></b><b> </b><span style="font-size:9.0pt"><span style="font-family:" verdana="">This study aimed to compare the effects of acetone and ethanolic extracts of <i>Plantago major</i> (<i>P. major</i>) on <i>S. mutans</i>. &nbsp;</span></span></span></span><br> <span style="font-size:13pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="font-family:" verdana=""><span style="color:#2e74b5">Materials and Methods:</span></span></span></b><span style="font-size:9.0pt"><span style="font-family:" verdana=""> In this <i>in vitro</i> study, the antibacterial effects of eight different concentrations of the acetone and ethanolic extracts of <i>P. major</i> seed were examined on <i>S. mutans</i>, and the minimum inhibitory concentration (MIC) and</span></span> <span style="font-size:9.0pt"><span style="font-family:" verdana="">minimum bactericidal concentration (MBC) of the extracts and the inhibition zone diameter were assessed.</span></span></span></span><br> <span style="font-size:13pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="font-family:" verdana=""><span style="color:#2e74b5">Results: </span></span></span></b><span style="font-size:9.0pt"><span style="font-family:" verdana="">The acetone extract of <i>P. major</i> seed showed a superior antibacterial effect compared with its ethanolic extract, demonstrating antibacterial effect on <i>S. mutans</i> at 1 mg/mL concentration by the cup plate method and a mean growth inhibition zone diameter of 6&plusmn;0.2 mm. the MIC and MBC of this extract were 0.5 mg/mL and 1 mg/mL, respectively. The ethanolic extract of <i>P. major</i> seed had an antibacterial effect on <i>S. mutans</i> with a concentration of 0.5 mg/mL by the cup plate method with a mean inhibition zone diameter of 8.3&plusmn;0.1 mm. The MIC and MBC of this extract were 2 mg/mL and 8 mg/mL, respectively. An increase in the diameter of inhibition zone was seen at higher concentrations of both extracts. The mean diameter of growth inhibition zone of chlorhexidine (CHX) was 24 mm.</span></span></span></span><br> <span style="font-size:13pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="font-family:" verdana=""><span style="color:#2e74b5">Conclusion:</span></span></span></b> <span style="font-size:9.0pt"><span style="font-family:" verdana="">The present results demonstrated the antibacterial activity of</span></span> <span style="font-size:9.0pt"><span style="font-family:" verdana="">ethanolic and acetone extracts of <i>P. major</i> seed on <i>S. mutans</i>. The acetone extract was more effective than ethanol extract on <i>S. mutans</i>. </span></span></span></span></td> </tr> </tbody> </table> </div> <div style="text-align: justify;"></div> Anti-Bacterial Agents, Ethanol, Acetone, Plantago major, Streptococcus mutans 88 94 http://jrdms.dentaliau.ac.ir/browse.php?a_code=A-10-875-1&slc_lang=en&sid=1 Sadighe Mozafar Se.mozafar@gmail.com 100319475328460011336 0000000164093435 No Department of Pediatric Dentistry, Faculty of Dentistry, Shahed University, Tehran, Iran Elham Majidi el.majid1371@gmail.com 100319475328460011337 0000000167470443 No Department of Restorative Dentistry, ‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌Zahedan University of Medical Sciences, Zahedan, Iran Ronak Bakhtiari rbakhtiari@sina.tums.ac.ir 100319475328460011338 0000000237785732 No Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mahdi Norouzi mnorouzi@tums.ac.ir 100319475328460011339 100319475328460011339 No Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Somayeh Kameli so.kameli@yahoo.com 100319475328460011340 0000000233956223 Yes Department of Pediatric Dentistry, Faculty of Dentistry, Shahed University, Tehran, Iran