Journal of Research in Dental and Maxillofacial Sciences

fa Comparison of Cytotoxicity of AH Plus and DC Canal SE Sealer After Final Setting Oral medicine Oral medicine Original article Original article <div> <table align="center" hspace="0" vspace="0"> <tbody> <tr> <td style="padding: 0in 12px; text-align: justify;" valign="top"> Background and Aim: This study aimed to compare the cytotoxicity of AH Plus and DC Canal SE sealer after final setting. Materials and Methods: In this in vitro, experimental study, human gingival fibroblasts (HGFs) were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 µg/mL streptomycin, and incubated at 37°C and 5% CO2. Cells at a density of 5000 cells/well were seeded in a 96-well plate for the methyl thiazolyl tetrazolium (MTT) assay. The cells were incubated with AH Plus and DC Canal SE sealers. Specimens of freshly mixed sealers were fabricated with 4 mm height and 10 mm diameter, and  incubated at 37°C for 24 hours for setting. Each specimen was incubated with 10 mL of diluted culture medium at 37°C and 5% CO2 for 24 hours. Finally, the solution was filtered using a 0.22-µm filter. Different dilutions (1, 1/2, 1/4, 1/8) were prepared, and cell viability was assessed at 24 and 72 hours by measuring the optical density of the solutions spectrophotometrically. Data were analyzed by ANOVA and Tukey’s test. Results: Cell viability in presence of all concentrations of AH Plus and DC Canal SE was significantly lower at 72 hours compared with 24 hours (P<0.001). Cell viability in presence of AH Plus was significantly higher compared with DC Canal SE at all concentrations and time points. Conclusion: Cell viability was higher in presence of AH Plus compared with DC Canal SE sealer in all concentrations and time points. </td> </tr> </tbody> </table> </div> Endodontics, Root Canal Obturation, Root Canal Filling Materials 91 97 http://jrdms.dentaliau.ac.ir/browse.php?a_code=A-10-815-1&slc_lang=fa&sid=1 AA Moshari Amirabbas.moshari@gmail.com 100319475328460010142 100319475328460010142 Yes Department of Endodontics, Dental Material Research Center, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran E Esnaashari Ehsanesna.dmd@gmail.com 100319475328460010143 100319475328460010143 No Department of Endodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran S Eskandarion s.eskandarion@gmail.com 100319475328460010144 100319475328460010144 No Dental Material Research Center, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran B Safari benyamin.safari96@gmail.com 100319475328460010145 100319475328460010145 No Private Dentistry Practice, Tehran, Iran