en Oral medicine Oral medicine Original article Original article <p style="text-align: justify;"><strong>Background and Aim:</strong> The rapid outbreak of coronavirus disease 2019 (COVID-19) in Wuhan, China has led to a public health crisis. Dental professionals have a major role in preventing the viral infection cycle. This study targeted to investigate the knowledge of 140 dental assistants in Tehran, Iran, about COVID-19.<br> <strong>Materials and Methods:</strong> A cross-sectional survey was conducted in Tehran, Iran, using a 27-item questionnaire, which consisted of 7 multiple-choice questions about the demographic information of the participants and 20 multiple-choice questions about their awareness. The data were analyzed with multivariable generalized estimating equations (GEE) using student t-test.<br> <strong>Results:</strong> A total of 140 participants (22 males and 118 females with a mean age of 29.40&plusmn;6.21 years) responded to the questionnaire. The majority of respondents (91.4%) mentioned cough and fever as the main symptoms of COVID-19 infection. Tooth extraction and root canal therapy were mentioned as emergency treatments according to 98.6% of respondents. 89.3% of respondents chose phone calls as the best screening tool. The knowledge and attitude of participants showed a significant correlation with their educational degree.<br> <strong>Conclusion:</strong> The knowledge of dental assistants about COVID-19 infection was fair. The participants&rsquo; awareness showed no correlation with their gender, work experience, place of occupation, and the specialty of the dentist that they assist.</p> COVID-19, Dental Care, Knowledge 8 13 http://jrdms.dentaliau.ac.ir/browse.php?a_code=A-10-807-1&slc_lang=en&sid=1 M Sohrabi Sohrabi.m22@gmail.com 10031947532846008205 10031947532846008205 No  Oncology Fellowship, Oral and maxillofacial surgery Dept, Faculty of Dentistry,Tehran Medical Sciences, Islamic Azad University, Tehran, Iran S Alirezaie Dr.somayealirezaie@gmail.com 10031947532846008206 10031947532846008206 No Oral Medecine Dept, Mebership of Dental Material Research Center, Faculty of Dentistry,Tehran Medical Sciences Sh Amini Shahinamini.dmd@gmail.com 10031947532846008207 10031947532846008207 Yes Private Practice, Tehran, Iran