%0 Journal Article %A Moshari, AA %A Esnaashari, E %A Eskandarion, S %A Safari, B %T Comparison of Cytotoxicity of AH Plus and DC Canal SE Sealer After Final Setting %J Journal of Research in Dental and Maxillofacial Sciences %V 7 %N 2 %U http://jrdms.dentaliau.ac.ir/article-1-315-en.html %R 10.52547/jrdms.7.2.91 %D 2022 %K Endodontics, Root Canal Obturation, Root Canal Filling Materials, %X Background and Aim: This study aimed to compare the cytotoxicity of AH Plus and DC Canal SE sealer after final setting. Materials and Methods: In this in vitro, experimental study, human gingival fibroblasts (HGFs) were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 µg/mL streptomycin, and incubated at 37°C and 5% CO2. Cells at a density of 5000 cells/well were seeded in a 96-well plate for the methyl thiazolyl tetrazolium (MTT) assay. The cells were incubated with AH Plus and DC Canal SE sealers. Specimens of freshly mixed sealers were fabricated with 4 mm height and 10 mm diameter, and incubated at 37°C for 24 hours for setting. Each specimen was incubated with 10 mL of diluted culture medium at 37°C and 5% CO2 for 24 hours. Finally, the solution was filtered using a 0.22-µm filter. Different dilutions (1, 1/2, 1/4, 1/8) were prepared, and cell viability was assessed at 24 and 72 hours by measuring the optical density of the solutions spectrophotometrically. Data were analyzed by ANOVA and Tukey’s test. Results: Cell viability in presence of all concentrations of AH Plus and DC Canal SE was significantly lower at 72 hours compared with 24 hours (P<0.001). Cell viability in presence of AH Plus was significantly higher compared with DC Canal SE at all concentrations and time points. Conclusion: Cell viability was higher in presence of AH Plus compared with DC Canal SE sealer in all concentrations and time points. %> http://jrdms.dentaliau.ac.ir/article-1-315-en.pdf %P 91-97 %& 91 %! Cytotoxicity of AH Plus and DC Canal SE sealers %9 Original article %L A-10-815-1 %+ Department of Endodontics, Dental Material Research Center, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran %G eng %@ %[ 2022