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Ex Vivo Comparison of File Fracture and File Deformation in Canals with Moderate Curvature: Neolix Rotary System versus Manual K-files
A Mesgarani, N Hoshyari, M Mousazadeh, A Kabbazzadeh,
Volume 7, Issue 4 (10-2022)
Abstract
Background and Aim: Cleaning and shaping is one of the important steps in endodontic treatment, which has an important role in root canal treatment outcome. This study evaluated the rate of file fracture and file deformation in Neolix rotary system and K-files in shaping of the mesiobuccal canal of maxillary first molars with moderate curvature.   
Materials and Methods: In this ex vivo experimental study, the mesiobuccal root curvature of maxillary first molars was measured by the Schneider’s method, and canal preparation was performed in 2 groups of 30 with Neolix rotary system and manual K-files. To determine the fracture rate of files, a file was used until it broke or deformed, and the number of canals cleaned by that file was recorded. The data were analyzed by the Mann-Whitney U test.  
Results: File fracture rate in the rotary group was slightly higher than that in the manual K-file group but, the frequency of file deformation in manual K-files was slightly more than that in the rotary group. There was no statistically significant relationship between file type and frequency of file fracture or deformation (P>0.05).
  Conclusion: Manual stainless steel K files and Neolix NiTi rotary files were the same in terms of file fracture and file deformation in preparation of canals with              moderate curvature.

Cytotoxicity of Two Calcium Silicate-Based Sealers and AH Plus Resin-Based Sealer for Human Gingival Fibroblasts
Narjes Hoshyari, Abbas Mesgarani, Mohammad Shokrzadeh Lamuki, Farzane Motafeghi, Seyed Jaber Mousavi, Milad Vaghar Mousavi,
Volume 8, Issue 3 (8-2023)
Abstract
Background and Aim: The main goal of root canal treatment is to three-dimensionally seal the root canal system. Since sealer may contact the periapical tissue, it should be biocompatible and safe for the body. This study aimed to assess the cytotoxicity of MTA Fillapex and Endoseal MTA calcium silicate-based sealers and AH Plus resin-based sealer for human gingival fibroblasts (HGFs).   
Materials and Methods:  In this in vitro study, extracts of AH Plus, Endoseal MTA, and MTA Fillapex were obtained, serially diluted 1:2, 1:4, and 1:8, and were exposed to HGFs. Cytotoxicity was assessed by the methyl thiazolyl tetrazolium (MTT) assay. Data were analyzed by ANOVA and Tukey’s test (alpha=0.05).
Results: No significant difference existed between AH Plus 1:2 and MTA Fillapex 1:2 concentrations regarding the cell viability percentage (P>0.05). However, the difference between these two sealers in other concentrations was significant (P<0.05). No significant difference existed in AH Pus 1:4, Endoseal MTA 1:2, and MTA Fillapex 1:2 in cell viability (P>0.05); however, the difference among other concentrations of the three sealers was significant (P<0.05). The difference among AH Plus 1:8, Endoseal MTA 1:4, and MTA Fillapex 1:4 and 1:8 concentrations was not significant (P>0.05) but the difference among other concentrations was significant (P<0.05). Endoseal MTA 1:8 showed the highest and AH Plus 1:2 showed the lowest cell viability.
Conclusion: Endoseal MTA in all concentrations had lower cytotoxicity than MTA Fillapex and AH Plus and resulted in higher viability of HGFs.

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