Journal of Research in Dental and Maxillofacial Sciences
J Res Dent Maxillofac Sci
Medical Sciences
http://jrdms.dentaliau.ac.ir
1
admin
2383-2754
10.61186/jrdms
en
jalali
1400
11
1
gregorian
2022
2
1
7
1
online
1
fulltext
en
Contamination of gutta-percha cones before and during clinical use
Oral medicine
Oral medicine
Original article
Original article
<div style="text-align: justify;"><strong>Introduction:</strong> Decontamination of gutta-percha (GP) cones is recommended before placement in the root canal system. However, the incidence of contamination is still a matter of debate. The present study aimed to evaluate the contamination of GP cones before and during clinical use by general dentists.<br>
<strong>Materials and Methods:</strong> In this in vitro study, 120 GP cones (#20) were examined for incidence of contamination. First, 30 GP packages were opened under aseptic laboratory conditions, and two cones were randomly selected for the laboratory tests. Next, the initially sampled packages were distributed among 30 general dentists and then they were asked to use them clinically for 7 days and then the packages were collected for the microbial tests. The collected cones were placed in tubes containing thioglycolate medium and incubated at 37℃ for 21 days. Bacterial growth was detected by presence of turbidity and comparison with the control groups. A sample was collected from the tubes showing turbidity and plated in blood agar and also underwent Gram-staining, followed by colony counting. Data were analyzed using the Chi-square and paired sample t-test (alpha=0.05).<br>
<strong>Results:</strong> None of the 60 samples from initial sampling of packages showed contamination. However, in secondary sampling after clinical use, 8.3% of GP samples were positive for contamination. There was a statistically significant difference in contamination of packages before and after clinical use (P= 0.02).<br>
<strong>Conclusion:</strong> Within the limitations of this study, it can be concluded that GP cones can become infected after opening the package and in the process of clinical use.</div>
Root Canal Therapy, Disinfection, Gutta-Percha, Sterilization
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47
http://jrdms.dentaliau.ac.ir/browse.php?a_code=A-10-802-2&slc_lang=en&sid=1
HR
Razeghi jahromi
hamid.rzgh@yahoo.com
100319475328460010110
100319475328460010110
No
Student Research Committee, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
M
Sadeghi
mostafa.sadeghi110@yahoo.com
100319475328460010111
100319475328460010111
No
Department of Operative dentistry, Faculty of Dentistry, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
F
Iranmanesh
Foad.iranmanesh@gmail.com
100319475328460010112
100319475328460010112
Yes
Department of Endodontic, Faculty of Dentistry, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
M
Tashakori
m_tashakori44@yahoo.com
100319475328460010113
100319475328460010113
No
Dept of Laboratory Sciences, Rafsanjan university of medical sciences, Rafsanjan, Iran.