Journal of Research in Dental and Maxillofacial Sciences
J Res Dent Maxillofac Sci
Medical Sciences
http://jrdms.dentaliau.ac.ir
1
admin
2383-2754
10.61186/jrdms
en
jalali
1395
4
1
gregorian
2016
7
1
1
3
online
1
fulltext
fa
In Vitro Comparison of the Effect of Ledermix® Paste and Green Tea Extract on the Concentration of Inflammatory Mediators
Oral & maxillofacial surgery
Oral & maxillofacial surgery
Original article
Original article
<p style="text-align: justify;"><strong>Background and aim:</strong> The purpose of this study is to compare the effect of Ledermix® paste and green tea extract on the concentration of inflammatory mediators.</p>
<p style="text-align: justify;"><strong>Materials and methods:</strong> In this in-vitro experimental study, first, the noncytotoxic concentrations of Ledermix® medicament and green tea extract were determined by MTT assay. Afterwards, the concentrations of IL-1β, IL-6, and TNF-α inflammatory mediators were assessed by ELISA test in the four experimental groups and also in the control group at different time intervals of 2, 24, and 48 hours and after 7 days. One-way analysis of variance (ANOVA) was used for statistical analysis of the obtained raw data followed by Tukey's post-hoc test for pair comparisons.</p>
<p style="text-align: justify;"><strong>Results: </strong>the noncytotoxic concentration of green tea extract and Ledermix® paste equaled 12 µg/ml and 0.25 µg/ml, respectively. Time interval had no significant effect on the concentration of the mediators. Generally, both substances significantly decreased the concentration of IL-1β and IL-6 inflammatory mediators in comparison with the control group.</p>
<p style="text-align: justify;"><strong>Conclusions:</strong> Green tea extract is less cytotoxic and more biocompatible than Ledermix® paste. Both Ledermix® paste and green tea extract expressed anti-inflammatory effects.</p>
Cytotoxicity, Green tea extract, Inflammatory mediators, Ledermix® paste
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http://jrdms.dentaliau.ac.ir/browse.php?a_code=A-10-347-1&slc_lang=fa&sid=1
M
Vatanpour
100319475328460010675
100319475328460010675
No
Assistant Professor, Endodontics Dept, Dental Branch of Tehran, Islamic Azad University, Tehran, Iran
A
Falah Doust
100319475328460010676
100319475328460010676
No
Assistant Professor, Endodontics Dept, Dental Branch of Tehran, Islamic Azad University, Tehran, Iran
G
Shojaee
100319475328460010677
100319475328460010677
No
Post Graduate student, Endodontics Dept, Dental faculty,Qom university of medical sciences,Qom,Iran
A
Jamshidian
jmdn.ali@gmail.com
100319475328460010678
100319475328460010678
Yes
Post Graduate student,Endodontics Dept, Dental Branch of Tehran, Islamic Azad University, Tehran, Iran.
A
Farasat
100319475328460010679
100319475328460010679
No
Pharmaceutical Sciences Branch,Islamic Azad University,Tehran,Iran