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Showing 6 results for Farhadi
F Shahsavari, Sh Ghasemi, S Farhadi, S Delavari, P Mazinani,
Volume 1, Issue 2 (Journal of Research in Dental & Maxillofacial Sciences spring 2016)
Background and aim: Increased rate of micronucleus in buccal mucosa cells and its correlation with carcinogenesis is worth consideration. Recently, occupation in dental laboratories has been proposed as a predisposing factor. Therefore, the present study aimed to evaluate the effect of occupational exposure on buccal mucosa cells of dental laboratory technicians.
Materials and methods: This historical-cohort study was conducted on 16 male dental laboratory technicians and 16 males were selected as the control group. All samples were matched according to age. The samples neither had any recent viral diseases nor were consumers of any specific medications. Cigarette smokers and alcoholics, individuals with risky occupations or with a history of radiotherapy were excluded. Buccal mucosa cells were sampled by use of a plastic spatula and were stained with Papanicolaou stain. Micronucleus frequency was evaluated under light microscope (400×). T-test was used for statistical analysis. Significance level was set at 0.05.
Results: Micronucleus frequency equaled 69±70 and 27±8.6 in case and control samples, respectively; which is 2.6 times higher in the case group. T-test showed that the difference in micronucleus frequency was significant between the two groups. (P<0.001)
Conclusions: the present study showed that the frequency of micronucleated cells in buccal mucosa of dental laboratory technicians is 2.6 times higher than that of the control subjects. Therefore, the mentioned occupation may increase the risk of induction of oral malignant transformations.
D Sadri, S Farhadi, M Mohammadi, Sm Seyedpour,
Volume 2, Issue 2 (Journal of Research in Dental & Maxillofacial Sciences spring 2017)
Background and aim: Following a correct biopsy protocol is crucial for accurate diagnosis of oral and maxillofacial lesions. Pre-analysis technical errors during preparation and submission of biopsies can jeopardize the diagnostic process and the related treatment plan. The aim of the present study was to evaluate pre-analysis technical errors in the biopsy samples submitted to an oral and maxillofacial pathology laboratory.
Materials and methods: In this descriptive study, 166 biopsy samples submitted to the oral and maxillofacial pathology laboratory of the dental branch of Islamic Azad University of Tehran in 2013 were assessed. 13 samples were excluded due to incomplete information. 153 samples were evaluated and compared to the standard criteria with regard to four fields including: biopsy request form, storage solution, container, and quality. The frequency of errors was reported with 95% confidence interval.
Results: No errors were detected in 19 samples (12.4%); whereas, 123 samples (80.4%) had incomplete biopsy request forms, 7 samples (4.6%) had been kept in improper storage media, 2 samples (1.3%) had unsuitable containers, and 2 biopsies (1.3%) had poor quality.
Conclusion: It seems that failure to submit a complete biopsy request form is the most common technical error, which indicates the need for periodic retraining and updates regarding the biopsy protocols.
M Manzarpour, M Homaie, S Farhadi,
Volume 2, Issue 3 (Journal of Research in Dental & Maxillofacial Sciences summer 2017)
Background and Aim: Oral squamous cell carcinoma (OSCC) accounts for approximately 3% of all cancers worldwide, and if diagnosed early, it has a five-year survival rate of around 85%; however, a late diagnosis may decrease the survival rate to 50%. Aberrant expression of several genes is associated with the hallmarks of OSCC including uncontrolled cell proliferation, poor differentiation, invasion, metastasis, and angiogenesis. The potential of molecular biomarkers for the diagnosis, prognosis, or monitoring of the treatment efficacy in OSCC has been extensively explored during the last decades. This study aimed to review the significance of salivary biomarkers in the treatment outcome of OSCC.
Materials and Methods: The articles in scientific databases including Google Scholar, ScienceDirect, Medline, and PubMed, published between 2004 and 2017, were searched by using relevant keywords including OSCC, biomarkers, diagnosis, prognosis and treatment outcome. Thirty-four articles were reviewed in this study.
Results: According to the findings of the reviewed studies, several salivary biomarkers including subcutaneous adipose tissue (SAT), interleukin-8 (IL-8), Cyfra 21-1, 8-hydroxy-2-deoxyguanosine, malondialdehyde, lactate dehydrogenase (LDH), Annexin A8, ErbB2, carcinoembryonic antigen (CEA), C-reactive protein (CRP), and salivary proteomic biomarkers might be used as indicators for the detection of oral cancer and premalignant oral disease (PMOD) and as a potential marker in the prognosis of OSCC.
Conclusion: Salivary biomarker analysis seems to be a major advancement in the diagnosis of OSCC, and it is a fast-developing field in scientific research. The results indicate that salivary biomarkers can be useful diagnostic and prognostic tools in OSCC.
S Farhadi, M Jolehar, K Babaeiha,
Volume 2, Issue 3 (Journal of Research in Dental & Maxillofacial Sciences summer 2017)
Background and aim: The ionizing radiation has been recognized to have deleterious effects on the DNA and induce cell death. Due to the importance of evaluating the DNA alterations in buccal mucosa cells induced by the ionizing radiation and by considering the limited volume of the samples used in previous studies, the present study aimed to assess the micronucleus (MN) incidence in the buccal mucosa of cone beam computed tomography (CBCT)-exposed patients of an oral and maxillofacial radiology center during 2016.
Materials and Methods: This descriptive study was conducted by using the target-based sampling. All the subjects, corresponding to the inclusion criteria, had been exposed to CBCT radiation according to their treatment plans. The specimens were scraped from the buccal mucosa of the participants by a damp spatula and were subsequently stained by Papanicolaou staining process. The percentage of the micronucleated cells was reported after evaluation under a light microscope. Also, the multiple linear regression (MLR) method was approached to statistically analyze the correlation between the MN frequency and the age and gender of the participants.
Results: The lowest and highest percentages of the MN frequency were respectively equal to 8.20% and 27.10% with the mean MN frequency of 17.68±4.98. Also, there was no significant correlation between the age and gender of the subjects and the MN frequency (P>0.05).
Conclusion: Based on the results of the present study, there was no significant correlation between the age and gender of the participants and the MN frequency.
S Farhadi, M Ghorbanpour, F Pourabed, A Lotfi,
Volume 3, Issue 1 (Journal of Research in Dental & Maxillofacial Sciences winter 2018)
Background and aim: Lichen planus (LP) is a chronic inflammatory disease with an uncertain pathogenicity. Recent reports have revealed that angiogenesis is effective in the pathogenesis and biologic behavior of lesions. Considering the limitations of the studies related to angiogenesis in oral LP (OLP) and the controversial results, the present study aimed to compare the expression of vascular endothelial growth factor (VEGF) in OLP and normal mucosa.
Materials and methods: In this case-control study, 4-µm sections were prepared from selected OLP (erosive type) blocks and normal mucosa samples for immunohistochemistry (IHC) staining with VEGF marker. VEGF expression was quantitatively assessed via counting the positive-stained cells. The mean percentages of positive-stained cells in each sample were categorized in three groups: score 0 (the presence of 10% or lower), score 1 (the presence of 10% to 50%), and score 2 (the presence of more than 50% of stained cells). T-test and Mann-U-Whitney test were used for statistical analyses.
Results: The mean percentage of VEGF expression was 39.8% in 35 OLP samples, and 20.5% in 20 normal mucosa samples. Twenty OLP samples (57%) had score 1, and 15 (43%) had score 2. Eighteen normal mucosa samples (90%) had score 1, and two (10%) had score 2. Also, score 0 was not reported in any of the samples. The percentages of VEGF expression in OLP and normal mucosa did not show any statistically significant differences (P=0.5). However, there was a significant difference between OLP and normal mucosa in VEGF expression score (P=0.012), and OLP samples had significantly higher scores.
Conclusion: According to the results, VEGF expression in erosive OLP samples was significantly higher than that in normal mucosa.
S Farhadi, F Shahsavari, K Alf,
Volume 3, Issue 2 (Journal of Research in Dental & Maxillofacial Sciences Spring 2018)
Background and Aim: It has been suggested that oral lichen planus (OLP) can be a potential premalignant lesion. Reports are also available on the role of bcl-2 and p53 proteins in malignant transformation of OLP into oral squamous cell carcinoma (OSCC). Due to controversies in these reports, the present study aimed to compare the expression of p53 and bcl-2 markers in OLP and OSCC.
Materials and Methods: In this experimental study, immunohistochemical (IHC) staining was performed on erosive OLP and well-differentiated OSCC samples using bcl-2 and p53 antibodies. One-thousand cells per slide were counted, and the results were reported as percentages. The results were scored, and Mann-Whitney U test was used to compare staining grades in the two lesions.
Results: Twenty-two OLP and 25 OSCC samples with a mean age of 49.3±15.8 years were evaluated. Bcl-2 expression was reported to be 16.27±8.95% in OLP and 16.4±22.9% in OSCC. Expression of p53 was 30.86±28.26% in OLP and 49.6±29.6% in OSCC. The difference in bcl-2 expression between the two lesions was not significant (P=0.266), whereas this difference was significant for p53 expression (P=0.02). The correlation coefficient between these two markers was reported to be 0.45 in OSCC and 0.1 in OLP.
Conclusion: According to the results, there was a significant difference in p53 expression between OLP and OSCC samples, whereas this difference was not significant for bcl-2. There was no significant association between the expressions of these two markers in the samples. Therefore, there does not seem to be a high malignancy potential for the studied OLP samples.