:: Volume 1, Issue 3 (Journal of Research in Dental & Maxillofacial Sciences summer 2016) ::
J Res Dentomaxillofac Sci 2016, 1(3): 8-13 Back to browse issues page
Evaluation of the Effect of Calcium Silicate Phosphate, Osteon, and Bio-Oss on Cell Viability and Cell Morphology of Human SaOS-2
S Sadatmansouri , N Ayubianmarkazi * , S Aghajeri , MR Delaram , B Iranpour
Associate professor Department of periodontology, Dental branch of Tehran, Islamic Azad University, Tehran, Iran. , naderayubian@yahoo.com
Abstract:   (1849 Views)

Background and Aim: Several reports have been published on the successful application
of various bone substitute materials (BSM). Appropriate physiologic and
histologic characteristics and reactions of these materials against host cells are critically
important. In this study, the biocompatibility of a new bone substitute material
has been evaluated.
Methods and Materials: In this experimental In vitro study, the biocompatibility
of silicate calcium phosphate, Bio-Oss and Osteon were compared by evaluation of
cell viability and differentiation rate of human osteoblast-like cell line (SaOS-2). No
graft material was used in the control group. Cell viability rate was evaluated by MTT
test after 1, 3 and 14 days of incubation. Inverted Light Microscope and SEM were
utilized for evaluation of cell morphology. MTT and cell morphology were analyzed
by ANOVA test in all groups.
Results: Cell viability of the control group equaled 0.453±0.016, while in the test
groups it equaled 0.453±0.016 for Bio-Oss, 0.439±0.011 for Osteon and 0.425±0.026
for silicate calcium phosphate. There was no significant difference between the control
and the test groups. Spindle form was the dominant SaOS-2 cell morphology in
all groups.
Conclusion: This study showed that calcium silicate phosphate has appropriate biocompatibility
comparable with that of Bio-Oss and Osteon.

Keywords: Saos-2, bone substitute, cell viability, cell morphology
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Type of Study: Original article | Subject: Periodontology

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Volume 1, Issue 3 (Journal of Research in Dental & Maxillofacial Sciences summer 2016) Back to browse issues page